Method for preparing antioxidant active fungus extracts

Abstract

The invention relates to a method for preparing antioxidant active fungus extracts. The method comprises the following steps of: adding papain or neutral protease or pectinase into mycelium according to a weight ratio of 1%, and adding distilled water for homogenate according to the proportion of a weight ratio of 1:10; after enzymolysis for 2 hours at the temperature of 45-50 DEG C and under the pH of 4.5-7.5, directly putting enzymolysis mixed liquor into water bath of 100 DEG C to be extracted for 2 hours, and collecting supernate in a centrifugation mode; after the supernate is concentrated, adding three times of volume of edible alcohol for precipitating for 24 hours at the temperature of 4 DEG C, and collecting sediments in a centrifugation mode; and after the sediments are frozen and dried by vacuum, obtaining freeze-dried powder of alcohol insoluble extracts with antioxidant activity. Compared with a hot water extraction method, the method for preparing antioxidant active fungus extracts has the advantages that the yield of alcohol insoluble extracts of the fungus mycelium can be increased by 10.0-74.1%, the total antioxidant activity of the extracts can be increased by 9.8-78.4%, the scavenging activity of superoxide anion radical can be increased by 48.5-192.2%, and moreover the operation time is obviously shortened and the energy consumption is reduced. The invention is a simple, high-yield and low-consumption method for preparing high antioxidant active fungus alcohol insoluble extracts.

Description

technical field [0001] The invention relates to the fields of food and health care products, biopharmaceuticals and enzyme engineering, in particular to a method for producing fungi with high antioxidant activity by using three different enzymes such as papain, neutral protease and pectinase. Background technique [0002] Modern studies have shown that free radicals in the human body are considered to be one of the important factors that cause oxidative damage to biological cells, and then cause tumors, aging, cardiovascular and cerebrovascular diseases and other diseases. Among the free radicals in the human body, super Oxygen anion free radicals and hydroxyl free radicals and other oxygen free radicals, superoxide anion free radicals are an important free radical produced in the metabolic process of life activities, and have a strong oxidation ability, and hydroxyl free radicals are the most active free radicals Radicals are also the most toxic free radicals. They can reac...

AI Technical Summary

Problems solved by technology

[0004] Although the use of enzymatic degradation of fungal cell walls can significantly increase the extraction yield of intracellular substances, however, the antioxidant bioactivity of fungal intracellular extracts produced by enzymatic methods has not yet been reported.

Our comparative study on a variety of enzymes shows that, compared with the hot water extraction method, different enzymes not only have a significant impact on the extraction rate of fungal mycelium intracellular substances, but also have a significant impact on the antioxidant activity of the extract. In-depth studies have shown that the antioxidant active substances in the extract are mainly alcohol-insoluble extracts, and, although some enzymes significantly increase the yield of alcohol-insoluble extracts, but at the same time cause the antioxidant activity of the obtained alcohol-insoluble extracts to decrease. This is obviously not conducive to the production of alcohol-insoluble extracts with high antioxidant activity by enzymatic methods, and will lead to a reduction in the efficacy of alcohol-insoluble extracts prepared by enzymatic methods, which seriously affects the treatment of diseases. When used in the development of health products It will also significantly reduce its health effects such as anti-oxidation, anti-aging, and improving health