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Method for extracting dragon fruit peel polysaccharides

A technology of pitaya peel and an extraction method, which is applied in the field of deep processing of fruits, can solve the problems of the destruction of the activity of pitaya polysaccharides, the reduction of the antioxidant properties of the pitaya polysaccharides and the ability of scavenging free radicals, etc. The effect of extraction efficiency

Active Publication Date: 2018-12-11
BENGBU COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The hot water extraction method is to use the characteristics of the best solubility of pitaya polysaccharides in hot water at about 120°C to extract it. However, at this temperature, the activity of pitaya polysaccharides will be destroyed to a certain extent. The antioxidant properties and ability to scavenge free radicals of fructose polysaccharides will be greatly reduced

Method used

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  • Method for extracting dragon fruit peel polysaccharides
  • Method for extracting dragon fruit peel polysaccharides

Examples

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Effect test

Embodiment 1

[0028] The extraction method of dragon fruit peel polysaccharide, described method comprises the following steps:

[0029] (1) Take 20g of clean dragon fruit peel, chop it up, add 240mL of water, the temperature of the water is 22°C, make a pulp, get the dragon fruit peel slurry, centrifuge, and take the supernatant;

[0030] (2) Add 3.5 times the volume of ethanol solution to the supernatant obtained in step (1), let stand for 100min, centrifuge, collect the precipitate, wash 3 times with ethanol solution, centrifuge, collect the precipitate, and obtain the pitaya peel crude polysaccharide;

[0031] (3) The pitaya peel crude polysaccharide obtained in step (2) is added to 240 mL of water at a temperature of 22° C., stirred and dissolved to obtain an aqueous solution of the pitaya peel crude polysaccharide;

[0032] (4) chloroform and n-butanol are uniformly mixed with a volume ratio of 3.5:1 to obtain the sevage reagent;

[0033] (5) get the pitaya peel crude polysaccharide ...

Embodiment 2

[0038] (1) Take 20g of clean dragon fruit peel, chop it, add 360mL of water, the temperature of the water is 28°C, make a pulp, get the dragon fruit peel slurry, centrifuge, and take the supernatant;

[0039] (2) Add 4.5 times the volume of ethanol solution to the supernatant obtained in step (1), let stand for 150min, centrifuge, collect the precipitate, wash 4 times with ethanol solution, centrifuge, collect the precipitate, and obtain the pitaya peel crude polysaccharide;

[0040] (3) adding the pitaya peel crude polysaccharide obtained in step (2) into 360 mL of water at a temperature of 28° C., stirring and dissolving to obtain an aqueous solution of pitaya peel crude polysaccharide;

[0041] (4) chloroform and n-butanol are mixed uniformly with a volume ratio of 4.5:1 to obtain the sevage reagent;

[0042] (5) get the pitaya peel crude polysaccharide aqueous solution that step (3) obtains and join in the sevage reagent that obtains in step (4), the volume ratio of descri...

Embodiment 3

[0047] The extraction method of dragon fruit peel polysaccharide, described method comprises the following steps:

[0048] (1) Take 20g of clean pitaya peel, chop it up, add 300mL of water, the temperature of the water is 25°C, beat for 5 minutes to obtain pitaya peel pulp, centrifuge, and take the supernatant;

[0049] (2) Adding 4 times of volume to the supernatant obtained in step (1) is a 65% ethanol solution, left to stand for 120min, and is centrifuged for 10min at a speed of 6000r / min, collects the precipitate, and uses a volume fraction of Wash with 65% ethanol solution for 3 times, centrifuge at a speed of 6000r / min for 10min, collect the precipitate, and obtain the crude polysaccharide from pitaya peel;

[0050] (3) The pitaya peel crude polysaccharide obtained in step (2) is added to 300 mL of water at a temperature of 25° C., stirred and dissolved to obtain the pitaya peel crude polysaccharide aqueous solution;

[0051] (4) chloroform and n-butanol are mixed unifo...

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Abstract

The invention provides a method for extracting dragon fruit peel polysaccharides. The method disclosed by the invention comprises the following steps: adopting a method for combining extraction with cold water of 22-28 DEG C and ethanol precipitation, adding the water of 22 to 28 DEG C into dragon fruit peel, pulping, and precipitating with ethanol; removing proteins in the crude polysaccharides by utilizing a sevage reagent, and refining the polysaccharides. In the process, the dragon fruit peel is not subjected to hot water treatment, the influence of the water temperature on the structure and activity of the dragon fruit peel polysaccharides is greatly reduced, the biological activity of the dragon fruit peel polysaccharides is remained to the greatest degree while ensuring the extraction efficiency, the ability of the polysaccharide for scavenging DPPH (1,1-Diphenyl-2-Picryl-Hydrazyl) free radicals is 91% or higher, the ability of the polysaccharide for scavenging peroxide anion radicals is 91% or higher, and the ability of the polysaccharide for scavenging hydroxyl radicals is 86% or higher. According to the method for extracting dragon fruit peel polysaccharides provided by the invention, the yield of the dragon fruit peel polysaccharides reaches 13.88%, and the retention rate of the dragon fruit peel polysaccharides in the discoloring process is 82% or higher.

Description

technical field [0001] The invention belongs to the field of fruit deep processing, in particular to a method for extracting pitaya peel polysaccharides. Background technique [0002] Pitaya, belonging to the cactus family, is native to Central America and later introduced to China. It is a tropical and subtropical fruit. Dragon fruit contains a large amount of plant polysaccharides. Studies have shown that plant polysaccharides have various biological activities, such as anti-oxidation, immune regulation, anti-tumor, hypoglycemic, antibacterial, etc. At present, there are literature reports on pitaya fruit stem and pulp polysaccharides, but there is no relevant report on the extraction of pitaya peel polysaccharides. The extraction methods of plant polysaccharides mainly include hot water extraction method, ultrasonic-assisted method and microwave-assisted method. The hot water extraction method is to use the characteristics of the best solubility of pitaya polysaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 钱时权方晓晖但德苗张斌马严兰
Owner BENGBU COLLEGE
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