41 results about "Thymosin beta-4" patented technology

The present invention provides embodiments which involve methods of providing a predetermined concentration of thymosin beta 4 (TB4) at a predetermined time, t, in a body portion of a live human patient. The methods can include determining a thymosin beta 4 treatment dosage (D) using Formula I: C=(A)D·t−B, wherein C is the predetermined concentration at time t, in ng / mL, D is the dosage of thymosin beta 4 administered in mg, t is the time elapsed after administration of dosage D in hours, A is about 30 to about 38, and B is about 0.5 to about 1; and administering the dosage (D) of thymosin beta 4 to the patient. Formula I may be, for example,C=(35.6)D·t−0.754  (Formula II).

The invention discloses a method for pyrolysis of thymosin beta-4. The method comprises the step of carrying out pyrolysis on thymosin beta-4 peptide resin to obtain crude peptide, wherein a mixed solvent of trifluoroacetic acid, thioanisole, dithioglycol, phenol, water, dimethyl sulfide and ammonium iodide is used as a pyrolysis reagent in the pyrolysis process. According to the method for pyrolysis of thymosin beta-4, the purity of crude peptide is greatly improved, meanwhile the total yield is greatly increased, the production cost is reduced, and the method is simple in process and convenient for industrial production.

The invention belongs to the technical field of medical biology, and particularly relates to a preparation method for a human thymosin beta 4 two-string body protein. A target protein with purity more than 98% can be obtained through the preparation method, the obtained human thymosin beta 4 two-string body protein has biological activity, the activity of the protein is determined through proliferation experiments and migration experiments, and results show that the human thymosin beta 4 two-string body protein can promote proliferation and migration of cells and the activity of the human thymosin beta 4 two-string body protein is superior to that of a chemical synthesis monomer. The method only uses one chromatographic column to complete separation and purification of the target protein,is simple in steps, rapid and low in cost and lays a foundation for further study and wide application of human thymosin beta 4.

The invention discloses a recombined Tbeta 4-BP5 fusion peptide, a gene, an engineering bacteria and an application. The recombined fusion peptide is prepared by fusing thymosin beta 4 with Fabricius bursa pentapeptide BP5 through a soft Linker. The recombined Tbeta 4-BP5 fusion peptide gene is inserted into an expression vector to transfer the escherichia coli so as to obtain the gene engineering bacteria for effectively express the Tbeta 4-BP5 fusion peptide; the Tbeta 4-BP5 fusion peptide is prepared through liquid cultivation and purification; and the thioredoxin of the fusion peptide is eliminated by using enterokinase with His tag at the N-end, and the fusion peptide is further subjected to affinity chromatography purification so as to obtain the single recombined Tbeta 4-BP5 fusion peptide. The recombined Tbeta 4-BP5 fusion peptide disclosed by the invention can be used as a novel polypeptide immunologic adjuvant which is used in match with vaccines, can effectively enhance the organism cell immune level and the body liquid immune level, and has wide application prospects.

The invention relates to one section of thymosin beta 4 derivatives with high bioactivity which can be used to repair cornea, skin, and heart injury.

The invention discloses a temperature-sensitive ophthalmic in-situ gel preparation with a suitable phase transition temperature, which contains different types of poloxamers, and the preparation contains poloxamer 407 and poloxamer 188. The in situ gel can be administered in a liquid state at room temperature and forms a gel on the surface of the eye. The invention can enhance the stability of the recombinant human thymosin β4 drug and prolong the efficacy and shelf life of the drug.

The present invention relates to a therapeutic agent for ophthalmic disease containing thymosin beta 4. The present invention is more effective in reducing xerophthalmia than an ophthalmic solution containing cyclosporine A, is less irritant to eyes than conventional ophthalmic solutions and is physiochemically safe.