Artificial dermis bracket and preparation method of artificial dermis bracket

A dermal and artificial technology, applied in the field of artificial dermal scaffolds and its preparation, can solve the problems of poor repair ability of artificial dermal scaffolds, achieve the effects of promoting wound healing, preventing infection, and achieving long-term effects

Active Publication Date: 2013-08-14
SHENZHEN LANDO BIOMATERIALS
View PDF6 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide an artificial dermal scaffold and its preparation method, to overcome the problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] It can be understood that, as a modification of the above-mentioned embodiment, the two-factor slow-release microspheres (slow-release antibiotics and slow-release skin growth factors) can also be selectively loaded on either the superficial layer or the deep layer, or loaded on the same layer. level. The steps mainly included in the preparation method of the artificial dermal scaffold of the present invention are specifically described as follows.

[0031] Step 1: Prepare slow-release microspheres loaded with functional factors, which may include but not limited to microspheres loaded with antibiotics and / or microspheres loaded with skin factors.

[0032]Among them, in the process example of preparing antibiotic-loaded microspheres in step 1, first take 0.4 mL of an ultrapure aqueous solution of 10-200 mg / mL antibiotics such as gentamicin or vancomycin or penicillin to 4 mL of PLA or PLGA or PGA Dichloromethane DCM solution with a concentration of 20~200mg / mL is emuls...

Example Embodiment

[0041] Example 1

[0042] A preparation method of a two-factor slow-release artificial dermis support is:

[0043] First, prepare antibiotic-loaded PLGA microspheres: take 0.4 mL of 10 mg / mL ultrapure aqueous solution of gentamycin to 4 mL of PLGA DCM solution, emulsify with an internal cutting homogenizer at a speed of 3000 rpm for 30 seconds, and prepare To obtain the inner emulsion (w / o); in 30mL of deionized water, add 0.3mL polysorbate-80, mechanically stir until it is uniformly dispersed, and obtain the outer aqueous phase; add the inner emulsion to the outer aqueous phase, and homogenize The double emulsion (w / o / w) was obtained by emulsifying at a speed of 3000 rpm for 30 seconds with a paddle machine; the double emulsion was stirred at a medium speed for 3 hours until the DCM was volatilized, and then left to stand overnight. After centrifugation, wash with water three times to obtain the suspension of PLGA microspheres loaded with antibiotics;

[0044] At the same ...

Example Embodiment

[0053] Example 2

[0054] A preparation method of a two-factor slow-release artificial dermis support is:

[0055] First, prepare antibiotic-loaded PLGA microspheres: take 0.4 mL of 200 mg / mL penicillin ultrapure aqueous solution to 4 mL of PGA DCM solution, and emulsify with an internal cutting homogenizer at a speed of 5000 rpm for 60 seconds to obtain an inner emulsion (w / o); in 30mL deionized water, add 0.3mL polysorbate-80, mechanically stir until uniformly dispersed, and obtain the outer water phase; add the inner emulsion into the outer water phase, and use an internal cutting type homogenizer to The double emulsion (w / o / w) was prepared by emulsifying at 8000 rpm for 90 seconds; the double emulsion was stirred at a medium speed for 3 hours until the DCM was volatilized and left to stand overnight. After centrifugation, wash with water three times to obtain the PGA microsphere suspension loaded with penicillin;

[0056] At the same time, prepare microspheres loaded w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Porosityaaaaaaaaaa
Porosityaaaaaaaaaa
Login to view more

Abstract

The invention relates to an artificial dermis bracket. The artificial dermis bracket comprises a three-dimensional porous structure with a deep layer and a shallow layer, wherein sustained release microspheres loaded with functional factors are introduced into the artificial dermis bracket, and comprise microspheres loaded with antibiotics and microspheres loaded with skin growth factors, and a gradient structure is formed by the three-dimensional porous structure of the artificial dermis bracket due to different porosities of the deep layer and the shallow layer. The preparation method of the artificial dermis bracket mainly comprises the steps of: preparing the sustained release microspheres loaded with the functional factors; preparing collagen solutions with two concentrations as matrix solutions; and mixing the sustained release microspheres loaded with the functional factors into the corresponding matrix solutions and molding the three-dimensional porous structure comprising the deep layer and the shallow layer so as to form the artificial dermis bracket with introduction of the sustained release microspheres loaded with the functional factors. The artificial dermis bracket provided by the invention has antibacterial activity and the property of promoting wound healing.

Description

technical field [0001] The invention belongs to the fields of biomedical material technology and biomedical engineering. More precisely, the present invention relates to an artificial dermal scaffold and its preparation method. technical background [0002] As a human organ, the skin has the functions of retaining moisture, breathing and preventing bacterial invasion. When the skin is damaged by trauma, burns, inflammation, etc., especially when a large area of ​​skin is seriously damaged, the wound should be protected immediately. If only a superficial or small area of ​​skin is damaged, new skin will regenerate itself. If a large area of ​​deep skin is traumatized, the skin cannot repair itself, and autologous skin grafting, such as stamp skin grafting, mesh skin grafting, and skin inlays, is usually required. Or use artificial skin products. The artificial skin products in the prior art all have insufficient ability of the skin to induce autologous skin tissue repair,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/54A61L27/56A61L27/60A61L27/24A61L27/22A61L27/20
Inventor 佘振定谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap