Micro-patterning nano-fiber material composited with medicine-carrying MOF and preparation method and application thereof
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A technology of micropatterning and nanofibers, which can be used in the fields of pharmaceutical formulations, fiber chemical characteristics, cellulose/protein conjugated artificial filaments, etc., and can solve problems such as increasing red blood cell production
Active Publication Date: 2019-07-09
SHANGHAI NORMAL UNIVERSITY
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However, excessive use of DMOG has side effects, such as promoting massive an...
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Embodiment 1
[0082] Example 1 Preparation of Micropatterned Nanofiber Membrane
[0083] (1) Preparation and morphological characterization of ZIF-67 (zeolitic imidazole framework) and ZIF-67 loaded with DMOG (dimethyloxalylglycine)
[0084] 1. Preparation of ZIF-67 nanoparticles
[0085] Dissolve 0.291g of cobalt nitrate hexahydrate and 0.66g of 2-methylimidazole in 15mL of methanol respectively, mix and stir at room temperature for 24h, centrifuge at 10000rpm for 5min to obtain the product, wash repeatedly with methanol, and dry in vacuum at 80°C for 24h to obtain ZIF-67 nanoparticles, its SEM, TEM, EDS, XRD, nitrogen adsorption and desorption curves are as follows figure 1 shown.
[0086] 2. Preparation of drug-loaded ZIF-67 nanoparticles
[0087] At 37°C, soak 25mg of ZIF-67 in 15mg / mL DMOG phosphate buffer solution (PBS, pH=7.4) for 3 days, centrifuge and wash, then vacuum-dry at 37°C for 24h to obtain loaded DMOG The ZIF-67 (DMOG@ZIF-67) nanoparticles, its XRD, nitrogen adsorption...
Embodiment 5
[0124] Example 5 Full-thickness wound repair experiment in vivo
[0125] Using STZ to induce 30 18g male BALB / c experimental mice to suffer from diabetes. After one week of induction, the blood sugar was measured and the blood sugar value increased and the value was stable at 250mg / dl, indicating that the mice were successfully induced into diabetic mice for subsequent experiments. .
[0126] Establish a full-thickness skin wound (circular, 8 mm in diameter) in the back area of diabetic mice, and implant the micropatterned nanofiber materials prepared in Example 1 in the mouse wound area and group them, respectively: blank Control group (Control), PL / G group, Z-PL / G group, DZ-PL / G group. On days 0, 3, 7, 9 and 11, digital cameras were used to record the wound healing changes in the trauma area at fixed distances and angles, such as Figure 8 a. according to Figure 8 A, the effect diagram of the simulated effect of wound repair on the back of diabetic mice Figure 8 B, ...
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Abstract
The invention belongs to the field of biological materials, and relates to a micro-patterning nano-fiber material composited with medicine-carrying MOF and a preparation method and application thereof. According to the material, hydrophilic composite electrostatic spinning nano fiber arranged in a staggered mode is adopted as a skeleton, the micro-patterning structure is arranged in order, and anopen three-dimensional through porous structure is distributed; the hydrophilic composite electrostatic spinning nano fiber is a mixture of a supporting biological compatible material, a hydrophilic biological compatible material and repair active molecule loaded metal organic frame nano-particles. The preparation method comprises the steps that organic solves of the metal organic frame nano-particles with uniformly dispersed loaded repair active molecules, the supporting biological active material and the hydrophilic biological active material are adopted as spinning solutions, and electro spinning is performed for preparation. According to the material, on the basis of the slow-release medicine carrier activity and biological tissue activity, the activity of cells in the engine body environment and micro-patterning nano-fiber material is collaborated and promoted, tissue repair and wound healing are facilitated, and the material is used for preparing a wound healing material.
Description
technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a micropatterned nanofiber material compounded with drug-loaded MOF, a preparation method and application thereof. Background technique [0002] Foot ulcers are one of the main complications of diabetes, and the wounds are protracted. In severe cases, toe / limb amputation is required, and the postoperative five-year mortality rate is as high as 50% to 68%. Normal skin wounds can generally self-repair in a timely and orderly manner, while diabetic wounds have insufficient angiogenesis and slow wound healing, which is mainly due to the decreased secretion of angiogenesis-promoting growth factors and the decreased expression of their corresponding receptors in high-glucose environments , the matrix deposition is reduced, and the microenvironment required for wound healing cannot be formed. [0003] Skin tissue engineering, which has emerged in recent years, uses synth...
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