Preparation method of spherical crystal calcined bone

Abstract

The invention discloses a preparation method of spherical crystal calcined bone. The preparation method comprises the following steps: firstly, taking an allogeneic bone, removing soft tissue, performing coarse cutting, soaking in a NaOH solution and an H2O2 aqueous solution to remove immunogen and part of impurities, then performing primary low-temperature calcination, soaking in a mixed solution of sodium tripolyphosphate and dicalcium phosphate after calcination, and finally performing secondary high-temperature calcination. The spherical crystal calcined bone prepared by the method has higher compressive strength and breaking strength, does not generate inflammatory response when being implanted into a body, and has high biocompatibility. According to the invention, a secondary calcination technology is adopted, and a key step of adding a mixed solution of sodium tripolyphosphate and dicalcium phosphate to soak is adopted in the secondary calcination, so that the prepared material is enhanced in strength, improved in biocompatibility and suitable for bone transplantation.

Description

Technical field [0001] The present invention belongs to the field of allogeneic bone preparation, and in particular, it is specifically involved in a preparation method of spherical crystal calciner. Background technique [0002] Bone defects caused by different reasons, clinicians need to choose high quality bone plant materials. The ideal bone graft repair material should have excellent orthodontic activity and should also have good biocompatibility. Commonly used graft materials include autologous bone, allogeneic bone, heterogeneous bones, and artificial bones. Autologous bone bones will cause secondary damage to the injured, and new damage will have the risk of loster blood, infection, and increase postoperative complications. In recent years, allogeneic bones have many applications, but the source is serious, and there is a risk of immunogenicity and dissemination of disease. The composition and structure of artificial bones are not as good as natural bones, often with poor...

AI Technical Summary

Problems solved by technology

In the chemical reagent immersion method, sodium hypochlorite solution has been widely used in the deproteinization treatment of xenograft bone in the early stage of bone tissue engineering, and the effect of deproteinization has also been confirmed, but there are still the following deficiencies: ①The material after deproteinization is often Chlorine-containing residues; ② Poor permeability, unable to ensure the uniformity of deproteinization inside and outside the processed bone; ③ It can be seen under the scanning electron microscope that it has a strong erosion effect on the cancellous bone scaffold

Ultrasonic decellularization alone usually cannot completely decellularize and is too strong and may damage the extracellular matrix (ECM)

In addition, there are methods such as α-galactosidase treatment, deep freezing, and repeated freezing and thawing that can be used to remove antigens, but the results are not satisfactory

[0004] Domestic heterogeneous bone materials include Jinshi Zhiguling from Tianjin, Jinguwei from Shanghai, Gurui from Xi’an, and Haiao bone powder from Yantai, etc., all of which have insufficient bone formation properties. Issues where stability or immunogenicity do not meet clinical needs

The most widely used in the domestic market is Geistlich Bio-Oss bone powder from Switzerland, which has stable properties and good bone-forming ability, but is expensive, and the specific parameters of its process are not clear, and many studies at home and abroad have failed for many years. Create xenogeneic bone products that exceed their performance

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