Modified gelatin based composite sponge and preparation method and application thereof

A composite sponge and gelatin technology, which is applied in the field of composite sponge materials and its preparation, can solve the problems of gelatin sponge with single components, single ingredients, and inflammatory reactions

Pending Publication Date: 2018-04-20
BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But above-mentioned sponge material finds to have following defect in use: 1) above-mentioned sponge material easily takes place relatively serious inflammatory reaction when using, mainly comes off after the chemical cross-linking agent used in the gelatin chemical modification method and cross-linking. Residual functional groups lead to; 2) the above-mentioned gelatin sponge components are relatively single and can only play a single effect. Therefore, a new preparation method for gelatin-based composite sponges is studied to avoid inflammatory reactions caused by cross-linking agents and play a comprehensive role in compounding. Efficacy is necessary

Method used

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  • Modified gelatin based composite sponge and preparation method and application thereof
  • Modified gelatin based composite sponge and preparation method and application thereof
  • Modified gelatin based composite sponge and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Preparation of thiol-modified gelatin synthesized by modification of bisuccinic acid bisacylcystine dicarbonyldiimidazole activated ester

[0066] Dissolve 10 g of cystamine dihydrochloride in 150 ml of distilled water, and magnetically stir the transparent solution. Add 4 mol / L sodium hydroxide to the above solution to adjust the pH of the solution to 9-10. Then add 13.3g of succinic anhydride, and continuously add 4mol / L of sodium hydroxide to keep the pH value of the solution at 8-10. 3. The white precipitated product was collected by filtration, and the filter cake was washed 3 times with 200ml of distilled water. Then it was placed in a blast oven at 50°C and dried to obtain about 13.4 g of white solid bisuccinic acid bisacylcystine.

[0067] Add 10g of bisuccinic acid bisylcystine and 60ml of anhydrous N'N-dimethylformamide into a 250mL beaker. After magnetically stirring at room temperature to dissolve, add 11.2g of carbonyldiimidazole. After about 2 ...

Embodiment 2

[0072] Example 2 Preparation of Modified Gelatin-Based Composite Sponge

[0073] Add 2.4 g of mercapto-modified gelatin (type A, from pigskin, Rossano, China) synthesized by modifying bisuccinic acid bisacylcystine dicarbonyldiimidazole activated ester into a 100 ml beaker, and mix with 40 ml pH The phosphate buffer solution of =7.4 dissolves, after dissolving, add 0.12g of hyaluronic acid and continue to stir to make it dissolve. Use a mechanical stirrer to stir for 5 minutes at a speed of 1600 rpm. At this time, a stable foam with a volume 5-6 times the volume of the original mixed solution is formed, and then add 2.5ml of double-armed polyethylene glycol acrylic acid with a concentration of 2.4%. Ester (2-arm-PEGDA) test solution, continue to stir for 1 minute, transfer the foam to a metal mold and place the mold in a 30°C oven for 5 minutes to complete the crosslinking of the foam. In order to make the foam continue to harden, the mold was transferred to a refrigerator at...

Embodiment 3

[0074] Embodiment 3 prepares modified gelatin-based composite sponge

[0075] Add 2.4 g of mercapto-modified gelatin (type A, from pigskin, Rossano, China) synthesized by modifying bisuccinic acid bisacylcystine dicarbonyldiimidazole activated ester into a 100 ml beaker, and mix with 40 ml pH The phosphate buffer solution of =7.4 dissolves, and after dissolving, add 0.06g of hyaluronic acid and continue to stir to make it dissolve. Use a mechanical stirrer to stir for 5 minutes at a speed of 1600 rpm. At this time, a stable foam with a volume 5-6 times the volume of the original mixed solution is formed, and then add 2.5ml of double-armed polyethylene glycol acrylic acid with a concentration of 2.4%. Ester (2-arm-PEGDA) test solution, continue to stir for 1 minute, transfer the foam to a metal mold and place the mold in a 30°C oven for 5 minutes to complete the crosslinking of the foam. In order to make the foam continue to harden, the mold was transferred to a refrigerator a...

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Abstract

The invention discloses modified gelatin based composite sponge and a preparation method and application thereof. The modified gelatin based composite sponge is prepared through modified gelatin, polysaccharide, a crosslinking agent polyethylene glycol derivative, a buffer salt solution and the balance water, wherein the modified gelatin is side chain sulfhydrylation modified gelatin, and the chemical structure of the modified gelatin is shown in formula (I) or (II); the crosslinking agent polyethylene glycol derivative is a polyethylene glycol derivative with sulfydryl reaction activity and is used for crosslinking sulfydryl of the modified gelatin; the polysaccharide comprises hyaluronic acid, chiton, chondroitin sulfate, heparin, alginic acid and a derivative thereof; the buffer salt solution is a phosphate buffer solution with pH of 5.0-8.0. According to the modified gelatin based composite sponge prepared through the method, the inflammatory response caused by a crosslinking agentand residual functional groups fallen off after crosslinking can be avoided; the biocompatibility is improved; massive modified gelatin based composite sponge can be quickly and efficiently prepared.The invention also provides a medical-modified gelatin based composite sponge and a preparation method thereof.

Description

technical field [0001] The invention relates to a composite sponge material and a preparation method thereof, in particular to a modified gelatin-based composite sponge, a preparation method and application thereof. Background technique [0002] Gelatin is a product of collagen treated with acid or alkali. It is a protein obtained by partial hydrolysis of collagen, which retains most of the excellent physical and chemical properties of collagen. Gelatin has excellent gelling properties, water holding properties, film-forming properties, emulsifying properties and foaming properties. Studies have found that gelatin has some biomedical properties: it has the properties of promoting cell adhesion and growth, has good compatibility with biological tissues, and has the functions of activating macrophages and hemostasis. In the field of medical materials, gelatin can be used in hemostatic materials, surgical wound repair, etc. [0003] Traditional wound treatment materials are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/04A61L24/08A61L24/00A61L24/10A61L27/18A61L27/20A61L27/22A61L27/50A61L27/56
CPCA61L24/001A61L24/0036A61L24/046A61L24/08A61L24/104A61L27/18A61L27/20A61L27/222A61L27/50A61L27/56A61L2400/04C08L71/02C08L5/08
Inventor 王昕宇王云云赵亚民宋文俊舒晓正高继宗
Owner BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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