Oil-in-water type compound vaccine adjuvant and method for preparing same

A vaccine adjuvant, oil-in-water technology, applied in the field of vaccines, can solve problems such as high cost and complicated preparation process, and achieve the effects of enhancing antibody level, simple and easy method, and complete vaccine absorption.

Inactive Publication Date: 2010-05-12
WENS FOOD GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Propolis adjuvant has been studied more on human vaccines (application number 2005100459649). Usually, it is first made into extract and then mixed with antigen to prepare nano-vaccine. However, due to the extremely complicated preparation process, the cost is relatively high

Method used

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  • Oil-in-water type compound vaccine adjuvant and method for preparing same
  • Oil-in-water type compound vaccine adjuvant and method for preparing same
  • Oil-in-water type compound vaccine adjuvant and method for preparing same

Examples

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preparation example Construction

[0047] The preparation method of above-mentioned adjuvant is as follows:

[0048] (1) under aseptic conditions, propolis and 70 volume % alcohol are mixed and dissolved according to the volume ratio of 1:4, and the propolis is broken by ultrasonication to obtain a propolis solution;

[0049] (2) Lecithin and medical alcohol are mixed and dissolved in a volume ratio of 1:1 to obtain a lecithin solution;

[0050] (3) After mixing vitamin E, propolis solution and lecithin solution in proportion, add them to sterilized Span and mineral oil to obtain the oil phase part;

[0051] (4) Mix isotonic phosphate-buffered saline with a pH of 7.2 and Tween after sterilization to obtain an aqueous phase;

[0052] (5) Pour the oil phase into the water phase and stir slowly, and fully mix to form an emulsion;

[0053] (6) Process the emulsion through a homogenizer to obtain the product.

[0054] The vaccine adjuvants prepared in Examples 1-5 are Nos. 1-5 respectively.

Embodiment 6

[0055] The stability measurement of embodiment 6 different formula adjuvants

Embodiment 1

[0056] The adjuvants prepared in Example 1 were stored in a refrigerator at 4°C and in an incubator at a constant temperature of 25°C, and the stability of the emulsion was observed by regular sampling (test results are shown in Table 2 and Table 3). The results showed that the adjuvant had good stability when stored at 4°C, and it also had good stability when stored at 25°C, especially the combination of two surfactants had the best effect. The No. 4 adjuvant showed a certain phase transition due to the similar ratio of dispersant and dispersed phase, and finally separated into two phases.

[0057] Table 2 Storage stability of different formulations of adjuvants at 4°C

[0058]

[0059] -- Indicates a homogeneous emulsion with no visible oil droplets observed under a microscope;

[0060] - Indicates that there are small oil droplets in the water phase, and a small amount of oil droplets are observed under a microscope;

[0061] ± indicates that there are small oil drople...

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Abstract

The invention discloses an oil-in-water type compound vaccine adjuvant and a method for preparing the same. The oil-in-water type compound vaccine adjuvant comprises an oil phase and a water phase, wherein the oil phase part comprises vitamin E, lecithin, propolis, mineral oil and a surface active agent; and the water phase part comprises an aqueous carrier, wherein the aqueous carrier comprises phosphate buffer solution and a hydrophilic surface active agent. The oil-in-water type compound vaccine adjuvant has the advantages of lowering viscosity of inactivated vaccines, making the vaccines more easily injected, making animals quickly generate immunoprotection with little local response, along with simple preparation method, low cost and the suitability for mass production of the vaccines.

Description

technical field [0001] The invention relates to the field of vaccines, in particular to an oil-in-water compound vaccine adjuvant and a preparation method thereof. Background technique [0002] It is known how to deliberately stimulate the body's immune response by administering specific antigens using vaccines, a procedure that induces an immune response state in which the body responds more rapidly and effectively when exposed to the antigen. Some antigens are only weakly immunogenic, and they do not elicit a sufficient immune response to effectively protect the organism. However, the immunogenicity of the antigen can be increased by mixing it with a substance called an adjuvant. Adjuvants increase the response to antigens by directly acting on the immune system, or by modifying the pharmacokinetic properties of the antigen, thus increasing the time for the antigen to interact with the immune system. [0003] At present, domestic veterinary vaccines mainly use oil adjuva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 宋延华杨明柳佘晓彬邓雨修
Owner WENS FOOD GRP CO LTD
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