Step-by-step tissue engineering bone building method

Abstract

The invention relates to the technical field of bone tissue engineering, and discloses a step-by-step tissue engineering bone building method. The method comprises the following steps of: (1) stent repair and pre-vascularization: building a tissue engineering bone sent at a bone defect part, and performing pre-vascularization; (2) later embedding of bone tissue engineering seed cells: after inflammatory reaction stage, embedding the seed cells, subjected to osteogenic induction, into the tissue engineering bone sent at the bone defect part based on growth-in time of capillaries, wherein the later embedding of bone tissue engineering seed cells in the step (2) is carried out 7-14 days later after the stent repair and pre-vascularization in the step (1). In the method provided by the invention, the sent promoting growth of vessels is firstly embedded, the seed cells for osteogenesis are embedded later, thus, a great segment of tissue engineering bone can be built quickly, application amount of the bone tissue engineering seed cells needed for repairing per unit volume of bone tissue is reduced greatly, and utilization rate of the seed cells is improved.

Description

technical field [0001] The invention relates to the technical field of bone tissue engineering, in particular to a step-by-step tissue engineering bone construction method for bone defect repair. Background technique [0002] Various types of bone defects throughout the body caused by factors such as trauma, tumors or aging are common diseases in the surgical system. Commonly used clinical treatment methods include autologous bone, allogeneic bone and biomaterial transplantation for repair, but there are problems such as demolishing the east to fill the west, potential disease transmission, and limited repair effect. In recent years, emerging bone tissue engineering technologies are expected to overcome the defects of these traditional technologies. [0003] The traditional bone tissue engineering technology is to expand the seed cells in a large amount in vitro, seed them on a three-dimensional degradable bio-scaffold with osteogenic activity, and implant them into the def...

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Problems solved by technology

2) The growth time of capillaries is too slow: usually, it takes 1 to 2 weeks for the capillaries to germinate and proliferate at the ends. A large number of apoptosis occurred, which did not play the role of mediating "crawling replacement"; and the size factor also determined that the microvessels could not quickly cover the entire large tissue engineered bone.

3) The "crawling replacement" effect of autologous osteoblasts is limited: Affected by their own age and health factors, the growth ability of individual cells varies significantly, and the distance that osteoblasts can crawl and replace varies, and large bone defects rely only on their own stumps Osteoblasts crawl and are difficult to fully repair

5) Insufficient strength of scaffold materials: Common large bone defects usually include limb bones and mandibles, which are supporting bone tissues with high stress requirements and strong compressive capacity

These methods have been proven to promote the growth of microvessels in the body faster than before, but the growth rate of blood vessels is still not enough

[0007] In summary, due to the large size of the defect and the variety of defect locations, the traditional tissue engineering bone technology has different repair effects on large bone defects, and it has always been unable to solve the problem of seed cells in the process of large-size bone repair. apoptosis problem

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