Step-by-step tissue engineering bone building method

A tissue engineering bone and construction method technology, applied in the field of bone tissue engineering, can solve the problems of slow ingrowth of microvessels, insufficient blood vessel growth rate, difference in cell growth ability, etc., and achieve the effect of maximizing the survival rate

Inactive Publication Date: 2015-07-01
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2) The growth time of capillaries is too slow: usually, it takes 1 to 2 weeks for the capillaries to germinate and proliferate at the ends. A large number of apoptosis occurred, which did not play the role of mediating "crawling replacement"; and the size factor also determined that the microvessels could not quickly cover the entire large tissue engineered bone.
3) The "crawling replacement" effect of autologous osteoblasts is limited: Affected by their own age and health factors, the growth ability of individual cells varies significantly, and the distance that osteoblasts can crawl and replace varies, and large bone defects rely only on their own stumps Osteoblasts crawl and are difficu...

Method used

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  • Step-by-step tissue engineering bone building method
  • Step-by-step tissue engineering bone building method
  • Step-by-step tissue engineering bone building method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Establishment of animal models

[0025] Since the rabbit radius model has been widely reported in various literatures as an orthotopic bone defect model, it is reported that a 1.5cm defect is a critical defect, and the animal cost is relatively economical. Therefore, an adult male white rabbit, 5 months old, with a radius defect of 1.5 cm was selected as the experimental animal model.

[0026] 2. Experimental grouping

[0027] Postplantation group: see below for specific experimental procedures;

[0028] Blank control group: without any treatment at the initial stage, only on the 14th day, 0.3ml of PBS was injected (no prevascularized seed cells were implanted, nor were osteoblast seed cells implanted);

[0029] Control group 1: traditional tissue engineering method, that is, 6 million bone marrow mesenchymal stem cells after osteogenesis induction were implanted at the initial stage;

[0030] Control group 2: no prevascularized seed cells were implanted at the in...

Embodiment 2

[0045] 1. Preparation of PCL polycaprolactone composite film:

[0046] (1) Take 15 grams of polycaprolactone raw material as a 3D printing material.

[0047] (2) First establish a geometric model through computer modeling software: design a planar network structure with holes, the diameter of the holes is 200 microns, and the thickness is 2 mm, and finally generate an STL format file.

[0048] (3) Print the solid model: input the STL format file obtained above into the 3D printer, set the printing parameters as required, including the height of the nozzle from the plate, the extrusion speed of the nozzle and the walking path of the nozzle, etc., and then print out the 3D model. That is to obtain a polycaprolactone composite membrane with a thickness of 2 mm and a pore diameter of 200 microns.

[0049] 2. Use the polycaprolactone composite film prepared in the above steps to wrap the bone defect site, and then carry out the distributed tissue engineering bone structure accordi...

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Abstract

The invention relates to the technical field of bone tissue engineering, and discloses a step-by-step tissue engineering bone building method. The method comprises the following steps of: (1) stent repair and pre-vascularization: building a tissue engineering bone sent at a bone defect part, and performing pre-vascularization; (2) later embedding of bone tissue engineering seed cells: after inflammatory reaction stage, embedding the seed cells, subjected to osteogenic induction, into the tissue engineering bone sent at the bone defect part based on growth-in time of capillaries, wherein the later embedding of bone tissue engineering seed cells in the step (2) is carried out 7-14 days later after the stent repair and pre-vascularization in the step (1). In the method provided by the invention, the sent promoting growth of vessels is firstly embedded, the seed cells for osteogenesis are embedded later, thus, a great segment of tissue engineering bone can be built quickly, application amount of the bone tissue engineering seed cells needed for repairing per unit volume of bone tissue is reduced greatly, and utilization rate of the seed cells is improved.

Description

technical field [0001] The invention relates to the technical field of bone tissue engineering, in particular to a step-by-step tissue engineering bone construction method for bone defect repair. Background technique [0002] Various types of bone defects throughout the body caused by factors such as trauma, tumors or aging are common diseases in the surgical system. Commonly used clinical treatment methods include autologous bone, allogeneic bone and biomaterial transplantation for repair, but there are problems such as demolishing the east to fill the west, potential disease transmission, and limited repair effect. In recent years, emerging bone tissue engineering technologies are expected to overcome the defects of these traditional technologies. [0003] The traditional bone tissue engineering technology is to expand the seed cells in a large amount in vitro, seed them on a three-dimensional degradable bio-scaffold with osteogenic activity, and implant them into the def...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/54A61L27/18A61L27/58B29C67/00B33Y10/00
CPCA61L27/20A61L27/365A61L27/3834A61L27/3847A61L27/46A61L27/56A61L2300/414A61L2300/64A61L2430/02C08L67/04C08L5/04
Inventor 张智勇汪振星吴鼎宇张占召李昱王进兵周权金鑫谢慧周广东张文杰刘伟曹谊林
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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