In the present invention are disclosed concatemers of concatenated expression cassettes and vectors that enable the synthesis of such concatemers. The
concatemer comprises in the 5′→3′ direction a cassette of
nucleotide sequence of the general formula [rs2-SP-PR-X-TR-SP-rs1]n wherein rs1 and rs2 together denote a functional
restriction site, SP individually denotes a spacer of at least two
nucleotide bases, PR denotes a
promoter, capable of functioning in a
cell, X denotes an expressible
nucleotide sequence, TR denotes a terminator, and SP individually denotes a spacer of at least two nucleotide bases, and n> / =2, and wherein at least a first cassette is different from a second cassette. The main purpose of these concatemers is the controllable and co-ordinated expression of large numbers of
heterologous genes in a single host. Furthermore, the invention relates to a
concatemer of cassettes of nucleotide sequences and a method for preparing the concatemers. In a further aspect, the invention relates to transgenic host cells comprising at least one
concatemer according to the invention, as well as to a method for preparing the transgenic host cells. Finally, the invention relates to a vector comprising a cassette of nucleotides, a method for preparing said vector, a nucleotide
library comprising at least two primary vectors each comprising a cassette of nucleotides, a method for preparing the
library.