The application provides a wearable sweat micro-fluidic analysis
chip and a preparation method and application thereof. The wearable sweat micro-fluidic analysis
chip is sequentially provided with a sweat inlet layer 1, a
branch channel layer 3 and a cover layer 5 from top to bottom. The sweat inlet layer is provided with a sweat inlet 6. The
branch channel layer 3 is provided with a detection chamber 10, a channel 17 and a valve chamber 9. The sweat inlet 6, the valve chamber 9, the channel 17 and the detection chamber 10 are sequentially communicated. The cover layer 5 is provided with a sweat outlet 14. A water droplet type
polymer valve is placed in the valve chamber 9. The opening time of the water droplet type
polymer valve can be flexibly controlled by simply adjusting the
polymer concentration. The sweat sample can be sequentially collected, and the
contact time of the sweat sample and the detection
reagent can be accurately controlled. A reliable premise is provided for subsequent accurate analysis. The application can be applied to the detection of cortisol and the detection of
uric acid.