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Protein sequence capable of inhibiting malignant tumors and application

A protein sequence, malignant tumor technology, applied in the field of genetic engineering, to achieve the effect of broad application prospects

Active Publication Date: 2021-02-05
广州研展科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still a lack of drugs based on the deletion or expression silencing of tumor suppressor genes, especially gene therapy drugs

Method used

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  • Protein sequence capable of inhibiting malignant tumors and application
  • Protein sequence capable of inhibiting malignant tumors and application
  • Protein sequence capable of inhibiting malignant tumors and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A protein sequence, which has the protein sequence shown in SEQ ID NO.1 (hereinafter referred to as p15N-p16C), and its source is as follows: the N-terminal of the human protein p15Ink4b sequence and the C-terminal of the p16Ink4b sequence.

[0037] The gene sequence of the expressed protein sequence SEQ ID NO.1 is shown in SEQ ID NO.2.

[0038] The protein sequence SEQ ID NO.1 was obtained by the inventors through creative efforts after comparative analysis of various proteins encoded by the chromosome 9p21 segment, such as p14Arf, p15Ink4b, p16Ink4a, MTAP, etc.

[0039] After a large number of comparative analysis experiments, the inventors found that only p15Ink4b and p16Ink4a have a strong inhibitory effect on tumor cells (the protein sequence of p15Ink4b is shown in SEQ ID NO.3, and the protein sequence of p16Ink4a is shown in SEQ ID NO. .4), the results are shown in the attached figure 1 .

[0040] figure 1 In bladder cancer cell line UMUC3, overexpression p...

Embodiment 2

[0090] Take 4-week-old nude mice Balb / C, and after one week of self-adaptation in the SPF animal room, they were randomly divided into two groups, one group was subcutaneously injected with SW780 bladder cancer cell line (control group), and the other was injected with p15N-p16C of the present invention. SW780 after lentiviral infection. Mouse tumors were photographed 20 days later. Figure 11 To verify the tumor suppressor effect of p15N-p16C for in vivo experiments in mice. It was found that the mice in the p15N-p16C treatment group did not form subcutaneous tumors, which indicated that p15N-p16C could effectively inhibit tumors.

[0091] In addition to the sequences listed in the above examples, the protein sequence SEQ ID NO.1 in the present invention can also be replaced by the following gene sequence (the following sequences are only listed, not exhaustive): SEQ ID NO.8. The above sequence can also achieve the purpose of expressing the protein sequence SEQ ID NO.1 after...

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Abstract

The invention belongs to the field of gene engineering, particularly relates to a protein sequence capable of efficiently inhibiting malignant tumors, and further relates to a gene sequence for encoding the protein sequence and application of the protein sequence. The application of the protein sequence as shown in SEQ ID NO.1 in preparation of a preparation for inhibiting the malignant tumors isthe key protected content of the invention. The protein sequence as shown in SEQ ID NO.1 is expressed by a gene sequence as shown in SEQ ID NO.2; and in addition, the gene sequence as shown in SEQ IDNO.2 is also the protected content of the invention. The protein sequence provided by the invention has the following advantages that the protein sequence has an excellent inhibition function in various tumor cells of bladder cancer, lung cancer and the like; the cancer inhibition effect is quick and obvious; and besides, the sequence provided by the invention is a human-derived sequence, does notcause rejection reaction, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to a protein sequence capable of inhibiting malignant tumors, a gene sequence encoding the above protein sequence, and an application of the above protein sequence. Background technique [0002] The technology of gene suppression of malignant tumors has been gradually applied, and it will gradually become an important supplement to the traditional method of suppressing malignant tumors due to its good effect and small side effects. [0003] From the perspective of the cause of malignant tumors, the main cause of cancer is gene mutation, including the amplification and overactivation of proto-oncogenes and the loss and silencing of tumor suppressor genes. For malignant tumors, in the traditional targeted therapy, most of the drugs are designed for the activation of proto-oncogenes, such as EGFR mutation, Myc mutation, ERBB mutation, etc., but these drugs are often prone ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P35/00C12N15/12C07K14/47
CPCA61K38/1709A61P35/00C07K14/47
Inventor 夏勇
Owner 广州研展科技有限公司
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