69 results about "Glycopolymer" patented technology

The invention discloses a cross-linkable amphiphilic natural polysaccharide and application thereof. Specifically, in particular, a double-bond and a hydrophobic chain are employed for dual modification of a natural polysaccharide polymer to obtain cross-linkable amphiphilic natural polysaccharide, and the cross-linkable amphiphilic natural polysaccharide is applied to loading and delivery of protein drugs. The amphiphilic natural polysaccharide provided by the invention can self-assemble into nanogel with protein loading capacity in an aqueous solution, and then an initiator is employed to activate the double-bond modifying natural polysaccharide to generate a free radical, thus triggering mutual crosslinking of the double-bond to form a new covalent bond, and the stable action force of the covalent bond is utilized to obtain the preparation so as to achieve efficient delivery of protein drugs.

The invention relates to a monosaccharide polymer enriching material and preparation thereof, and application of the monosaccharide polymer enriching material to enrichment of glycopeptides. Functional monosaccharide monomers are copolymerized onto a main polymer chain by using a Schiff-base condensation method and are further grafted onto a matrix material so as to obtain a sugar-responsive polymeric material. The monosaccharide polymer enriching material is applied to enrichment and/or selective separation of glycopeptides. The matrix material modified by a polymer is used for contacting with enzymatic glycoprotein hydrolysate for enrichment of glycopeptides. Specifically, a solid-phase extraction (SPE) mode or a dispersive solid-phase extraction mode is employed; and in a SPE mode, when the matrix material modified by the monosaccharide polymer is used for enriching glycopeptides, the enzymatic glycoprotein hydrolysate is loaded into a SPE instrument with the matrix material modified by the monosaccharide polymer as a filling material, then an eluent is used for flushing to remove non-glycopeptides, and glycopeptides are separated from eluate. The monosaccharide polymer enriching material prepared in the invention has the characteristics of high selectivity, high flux and the like in separation and enrichment of glycopeptides and can realize selective enrichment of glycopeptides.

The invention discloses a method for preparing xylooligosaccharide from corncobs through microwave-assisted hydrothermal treatment. The xylooligosaccharide is a functional glycopolymer formed by combining 2-6 xylose molecules by beta-1,4 glucosidic bonding; the method of the microwave-assisted hydrothermal treatment is that water is taken as a reaction solvent, and a complex three-dimensional network structure of cell walls of the corncobs is efficiently and rapidly broken by virtue of a microwave-assisted technology, so that hemicelluloses are dissolved out and subjected to hydrolysis reaction to generate the xylooligosaccharide; the specific steps for preparing xylooligosaccharide from the corncobs through the microwave-assisted hydrothermal treatment are as follows: crushing the corncobs into small granules, extracting by an organic solvent, filtering, washing by purified water, drying, and then carrying out ball-milling; adding a sample subjected to the ball-milling and the water in a reaction kettle, carrying out ultrasonic treatment and reacting in a microwave; and concluding the reaction, cooling, carrying out suction filtration to obtain filtrate, distilling and concentrating the filtrate, and carrying out freeze-drying to obtain the xylooligosaccharide. The method for preparing xylooligosaccharide from the corncobs through the microwave-assisted hydrothermal treatment has the advantages of being rapid, efficient, environmentally friendly, and the like.

The invention relates to the field of polymer monolithic column preparation, in particular to a double-channel hydrophilic bicontinuous polymer monolithic column and a preparation method thereof. A self-made amphiphilic diblock sugar-containing polymer is used as a surfactant to control a continuous phase emulsion, and the block polymer is also involved in the preparation of an initiator or a chain transfer agent for the polymerization of the monolithic column. With the combination of a small molecular pore-forming agent and through one-step polymerization of an active emulsion, the double-channel hydrophilic bicontinuous polymer monolithic column is obtained. According to the prepared double-channel hydrophilic bicontinuous polymer monolithic column, the skeleton of the monolithic columnis polystyrene, which has high mechanical strength and good chemical stability, and the outer surface of the skeleton is a sugar-containing polymer and has hydrophilicity and is easy to derive. The monolithic column pores penetrate, have two pores of super macropores and mesopores, and have wide application space in the fields of cell culture, enzyme immobilization and rapid separation and purification of proteins.

The invention discloses sugar-containing photodynamic therapy nano particles with blood stability and target ability and a preparing method of the sugar-containing photodynamic therapy nano particles. The nano particles are formed by wrapping photosensitizer micromolecules with a synthesized copolymer of DMA and DMAEMA, and wrapping the outer layers of nano particles with glycopolymer layers, wherein the average particle size of the nano particles ranges from 180 nm to 300 nm. The preparing method of the sugar-containing photodynamic therapy nano particles comprises the steps that the copolymer of DMA and DMAEMA is subjected to self-assemblage in water to wrap the photosensitizer micromolecules to form the nano particles, the nano particles are mixed with a glycopolymer solution with an end group serving as carboxyl, and the glycopolymer is aggregated on the surfaces of the nano particles through electrostatic interaction to form the sugar-containing photodynamic therapy nano particles. According to the method, operation is easy and convenient, and various photodynamic reagents can be wrapped. The adopted polymer shows electropositivity and can be combined with various glucide polymers with negative electricity, meanwhile, the glycopolymer is aggregated on the surfaces of the nano particles, and thus blood stability, target ability and biocompatibility are achieved.

The invention relates to a light-weight composite corundum refractory aggregate, and a preparation method for the light-weight composite corundum refractory aggregate. According to the technical scheme, the preparation method comprises the following steps: taking 80-95 wt% of gamma-Al2O3 fine powder, 1-10 wt% of Al(OH)3 micro powder, 1-10 wt% of a glycopolymer and 0.1-5 wt% of an antifreeze protein as raw materials, adding 20-60 wt% of water, and conducting wet grinding; pelleting slurry obtained after wet grinding into particles with the grain sizes being 1-2.5 mm through a disk, freezing, drying, and keeping the temperature for 3-6 h at the temperature of 300-800 DEG C to obtain porous corundum particles; then taking 80-97 wt% of alpha-Al2O3 micro powder, 2-10 wt% of nanometer aluminium oxide sol, and 1-5 wt% of MgO micro powder as mixtures, adding 30-50 wt% of organic alcohol, and conducting mixed grinding; pelleting the slurry obtained through mixed grinding and the porous corundum particles into particles of which the grain sizes are 3-10 mm, drying, and sintering for 1-5 h at the temperature of 1,700-1,900 DEG C, so as to obtain the light-weight composite corundum refractory aggregate. The light-weight composite corundum refractory aggregate product prepared by the invention is low in volume density, high in closed porosity, and high in slagging corrosion resistance.

The invention relates to a preparation method of glycopolymer with lateral chains containing heterogeneous sugar units. The preparation method includes: using 2-amino-2-methyl-1,3-propanediol as the raw material, using BOC to perform amino protection, then allowing the 2-amino-2-methyl-1,3-propanediol to have Williamson etherification reaction with propargyl bromide to prepare a compound with terminal alkyne, using an acetyl protected carbohydrate compound containing azido as the raw material to prepare a compound containing heterogeneous sugar through click chemical reaction, removing BOC protection, polymerizing with RAFT, using modification after polymerization to prepare glycopolymer with lateral chains containing heterogeneous sugar units, and then removing OAc protection to prepare glycopolymer containing heterogeneous sugar units. Compared with the prior art, the preparation method is simple in polymer monomer synthesizing method and controllable in polymer lateral chain structure, and the prepared glycopolymer is controllable in molecular weight, narrow in molecular weight distribution, good in water solubility and applicable to fields such as high polymer materials and biological medicine.

The invention discloses a preparation method and application of a glycopolymer. According to the preparation method, the combination of active ester exchange and active free-radical polymerization reaction at room temperature is utilized for the first time to successfully prepare the glycopolymer with controllable molecular weight and narrower molecular weight distribution by a one-step one-pot process. The invention provides a simple effective way for synthesizing the glycopolymer. The application method comprises the following steps: dialyzing the glycopolymer, carrying out freeze-drying, mixing the freeze-dried glycopolymer with an AgNO3 solution, and preparing the fluorescent sugar-containing silver nano cluster by using the glycopolymer as a stabilizer. The method for preparing the glycopolymer is also applicable to the preparation of glucose, galactose and other glycopolymers.

The invention relates to a method for detecting contaminants of glucose polymers, said contaminants being capable of acting in synergy with one another so as to trigger an inflammatory reaction, characterized in that it comprises an in vitro inflammatory response test using modified cell lines.

The invention relates to the technical field of biopolymer, and provides a double-component high-strength hydrogel and a preparation method thereof. The double-component high-strength hydrogel is prepared through mixing a glycopolymer and an N-isopropylamine acrylamide-o-hydroxymethylbenzeneboronic acid half ester random copolymer. The repeating unit of the glycopolymer is galactose connected through 6-site hydroxyl. According to the preparation method, the hydrogel is prepared according to a principle that glycopolymer and o-hydroxymethylbenzeneboronic acid half ester polymer can form dynamic covalent bond. The preparation method is simple to operate. The prepared hydrogel has high strength, and the adopted polymers are easy to synthesize. The hydrogel has glucose responsiveness, and can be used as a controlled release carrier of insulin. Therefore, a novel idea is provided for painless treatments of diabetes.

Poly(ethylene oxide) (PEO) glycodendrimers can serve as multivalent inhibitors of selectin-mediated leukocyte recruitment regulating inflammatory response. Disclosed are compounds and methods relating to functionalized branched glycopolymers. In embodiments, the compounds are capable of modifying cell adhesion events and inflammatory conditions. In a particular embodiment, a multi-arm PEO polymer with sulfated lactose end groups can specifically inhibit interactions involving L-selectin. Also disclosed are methods of synthetic preparation and use.

Provided are methods and compositions for treating an individual with cancer or infectious disease. Multivalent Dectin-2 stimulating agents are provided that include: (a) an agent that binds to Dectin-2 and stimulates Dectin-2 signaling; and (b) an antibody and/or an immunomodulatory agent, wherein (a) and (b) are conjugated to one another. In some cases, (a) is a mannobiose glycopolypeptide that binds to Dectin-2. In some cases (b) is a stimulatory ligand for a TLR (e.g., TLR7, TLR8, TLR7/8, TLR2, and the like). Methods of treating an individual with cancer and/or an infectious disease can include administering to the individual an effective amount of a Dectin-2 stimulating composition. In some cases, the Dectin-2 stimulating composition comprises a Dectin-2 stimulating glycopolymer. In some cases the Dectin-2 stimulating composition comprises a multivalent Dectin-2 stimulating agent.

The invention relates to a three-arm mannose derivative and a preparation method thereof through combination with double-click chemistry. Firstly, a three-arm terminal alkene/ alkyne compound and [alpha]-D-azide mannose ([alpha]-Man-N3) are used for carrying out a CuAAC reaction between end group alkyne and azide to generate a rigid triazole group; then, the rigid triazole group and [alpha]-D-sulfydryl mannose ([alpha]-Man-SH) carry out a Thiol-ene reaction between end group alkene and the sulfydryl to generate a flexible thioether bond; and then, under an acidic condition of trifluoroacetic acid, protection of t-butyloxycarboryl is removed to obtain one series of three-arm mannose derivatives. Compared with the prior art, the invention utilizes the advantages of the click chemistry reaction to innovatively synthesize three kinds of three-arm mannose derivatives with different structures so as to be favorable for jointly utilizing RAFT polymerization in the later stage to prepare a sugar-containing polymer of which the side chain contains tri-functional mannose and perform an important guidance meaning for researching the influence of the rigidity and the flexibility of different branched chains of the polymer of which the side chain contains three-arm mannose for the biological characteristics.

The invention provides a preparation method and application of a branched sugar-containing polymer nanoparticle as shown in a formula I. The branched sugar-containing polymer prepared by the preparation method disclosed by the invention has good self-assembly performance and tumor microenvironment responsiveness. The nanoparticle formed by self-assembly of the branched sugar-containing polymer can significantly improve the pharmacological properties of a small-molecule contrast agent, so that the nanoparticle has a longer blood half-life period, more tumor site accumulation, a higher tumor signal enhancement degree, a longer enhancement duration and better tumor enhancement specificity. The branched sugar-containing polymer nanoparticle prepared by the invention can be rapidly taken in by cells, release drugs in a tumor microenvironment, has long in-vivo circulation time and targeting property, and can significantly improve in-vivo distribution of small molecular drugs, so that the drugs are effectively accumulated at tumor sites, tumor growth is significantly inhibited, and the nanoparticle has good biological safety, and has huge development potential and wide application prospects in the field of tumor diagnosis and treatment integrated medicines.

The present invention is an enzyme-decomposable polymer compound having recyclable decomposition products. The polymeric compound is a glycopolymer of a saccharide having only glucopyranose rings and a second component. In addition, the glycopolymer may contain molecular chains having at least one type of repeating unit, which chains are crosslinked by a saccharide having only glucopyranose rings.

The invention relates to a Pseudomonas aeruginosa detection membrane and a preparation method thereof and a method for detecting bacteria, and belongs to the technical field of biological detection. The Pseudomonas aeruginosa detection membrane comprises a substrate layer, and comprises a detection layer, the detection layer is disposed on the substrate layer, the substrate layer comprises a nanofiber membrane, the detection layer comprises a fluoroboron fluorescent molecule functionalized glycopolymer and a phenylboronic acid polymer, wherein the fluoroboron fluorescent molecule functionalized glycopolymer and the phenylboronic acid polymer are uniformly and alternately assembled on the surface of the nanofiber membrane by layer-by-layer self-assembly. The detection membrane is not only low in cost but also capable of quantitatively detecting Pseudomonas aeruginosa.

The invention discloses a glycopolymer in achyranthes bidentata as well as a preparation method and application thereof. In the method, the achyranthes bidentata is used as a raw material, and is subjected to water-extraction alcohol precipitation and alkaline-extraction alcohol precipitation, so that polymers at all the parts of the achyranthes bidentata are obtained. The extraction method is implemented under a relatively gentle condition, and components of the glycopolymer are intactly preserved. The achyranthes bidentata glycopolymer has a remarkable effect at the aspect of anti-osteoporosis symptom; in addition, a glycopolymer pure product is obtained through separation and purification processes including Sevag method deproteinization, DEAE cellulose column chromatography, Sephacryl column chromatography and the like, and structural analysis shows that the achyranthes bidentata glycopolymer obtained through the method comprises polyose and oligose. Through the adoption of the achyranthes bidentata glycopolymer as well as the preparation method and application thereof, a basis is provided for the application of the achyranthes bidentata glycopolymer in the fields of health care products, medicines and the like.

The present invention relates to an antibody having specificity for an immunogenic determinant consisting of the pentasaccharide repeating unit of the Clostridium difficile glycopolymer PS-I: α-L-Rhap-(1→3)-β-D-Glcp-(1→4)-[α-L-Rhap-(1→3)]-α-D-Glcp-(1→2)-α-D-Glcp or a fragment thereof. Said antibody is able to prevent and treat diseases caused by C. difficile. The present invention further pertains to a method of treating or preventing a disease caused by the pathogen Clostridium difficile, which comprises administering to a subject said antibody or a vaccine composition comprising said antibody.

The invention relates to a synthesis method of a highly ordered dendronized heterogeneous glycopolymer containing various glycosyl groups. The method comprises the following steps: (1) adding anhydrous triethylamine into a dichloromethane solution of pentafluorophenol under an ice bath condition, then adding acryloyl chloride for reaction, and after the reaction is finished, washing, drying and purifying to obtain a compound PFPA; (2) carrying out polymerization reaction on the compound PFPA, a chain transfer agent and an initiator to obtain a polymer pPFPA; (3) mixing the polymer pPFPA with dendronized glycosamine, and carrying out ammonolysis reaction to obtain a glycopolymer; and (4) adding the glycopolymer into a sodium methoxide/methanol solution to react, and purifying after the reaction is finished, thereby obtaining the target product, namely the dendronized heterogeneous glycopolymer containing various glycosyl groups. Compared with the prior art, the method has the advantages that the advantages of RAFT polymerization and post-modification are combined, the glycopolymer with the ordered structure is obtained, particularly the regular heterogeneous glycopolymer is obtained, and a universal platform and the like are provided for preparation of the functional glycopolymer.