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Hydrogel based on gamma-polyglutamic acid and epsilon-polylysine cross-linked polymer and preparation method thereof

A technology of cross-linked polymers and polylysine, applied in bandages, absorbent pads, medical science, etc., can solve problems such as poor biocompatibility, poor biocompatibility of cross-linking agents, and limited application in the medical field

Active Publication Date: 2014-03-26
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chinese invention patents CN1629220A and CN101891954A disclose methods for preparing hydrogels using polyglutamic acid as the main raw material, both of which use glycidyl ether crosslinking agents. Non-biological source makes its biocompatibility poor. It is generally used in farmland water retention machines, environmental protection flocculation, etc., and its application in the medical field is greatly limited.
Chinese patent CN102585303A discloses a method for preparing hydrogels using polylysine as a raw material. The chitosan / polylysine hydrogels prepared by the invention are composed of polysaccharides / polypeptides of bionic natural extracellular matrix and structure, it is expected to be used as an injectable material as a tissue adhesive, but in the preparation process, chitosan and polylysine need to be chemically modified separately, and the synthesis steps are cumbersome
[0007] In summary, there are reports on the preparation of hydrogels using polyglutamic acid and polylysine as raw materials, but there are disadvantages such as poor biocompatibility of crosslinking agents and cumbersome preparation steps.

Method used

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  • Hydrogel based on gamma-polyglutamic acid and epsilon-polylysine cross-linked polymer and preparation method thereof
  • Hydrogel based on gamma-polyglutamic acid and epsilon-polylysine cross-linked polymer and preparation method thereof
  • Hydrogel based on gamma-polyglutamic acid and epsilon-polylysine cross-linked polymer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061]Dissolve 4.0 g of γ-polyglutamic acid (1 million to 1.2 million Daltons, containing 0.031 mol carboxyl group) in 50 ml of 0.1 mol / L MES buffer (pH 5.0) at room temperature, and stir until a clear solution is formed. Dissolve 1.78g ε-polylysine (3000-4500 Daltons, containing 0.014mol amino group) in 50ml 0.1mol / L MES buffer (pH5.0) at room temperature, drop ε-polylysine solution drop by drop Add it to the polyglutamic acid solution and stir to mix the solution evenly. Add 4.17g (0.0217mol) of EDC and 2.50g (0.0217mol) of NHS to the mixture of γ-polyglutamic acid and ε-polylysine to control the carboxyl group contained in γ-polyglutamic acid: ε- The molar ratio of amino groups contained in polylysine: EDC: NHS is 1: 0.45: 0.7: 0.7, reacted in ice bath for 30 minutes, and then reacted at room temperature for 2 hours to form a hydrogel. The formed hydrogel was placed in a dialysis bag, dialyzed in deionized water to swelling equilibrium, and then freeze-dried or vacuum-drie...

Embodiment 2

[0063] Dissolve 5.0 g of γ-polyglutamic acid (1 million to 1.2 million Daltons, containing 0.039 mol carboxyl group) in 50 mL of 0.1 mol / L MES buffer (pH 5.0) at room temperature, and stir until a clear solution is formed. Dissolve 1.99g ε-polylysine (3000-4500 Daltons, containing 0.0156mol amino group) in 50mL0.1mol / L MES buffer (pH 5.0) at room temperature, drop ε-polylysine solution drop by drop Add it to the polyglutamic acid solution and stir to mix the solution evenly. Add 4.49g (0.023mol) EDC and 5.08g (0.023mol) sulfo-NHS to the above-mentioned mixture of γ-polyglutamic acid and ε-polylysine to control the carboxyl groups contained in γ-polyglutamic acid: The molar ratio of amino groups contained in ε-polylysine: EDC: Sulfo-NHS is 1: 0.4: 0.6: 0.6, reacted in ice bath for 30 min, and then reacted at room temperature for 2 h to form a hydrogel. The formed hydrogel was placed in a dialysis bag, dialyzed in deionized water to swelling equilibrium, and then freeze-dried o...

Embodiment 3

[0065] Dissolve 6.0 g of γ-polyglutamic acid (1 million to 1.2 million Daltons, containing 0.047 mol of carboxyl groups) in 50 mL of 0.1 mol / L MES buffer (pH 5.0) at room temperature, and stir until a clear solution is formed. Dissolve 3.0g ε-polylysine (3000-4500 Daltons, containing 0.0235mol amino group) in 50mL0.1mol / L MES buffer (pH 5.0) at room temperature, drop the ε-polylysine solution drop by drop Add it to the polyglutamic acid solution and stir to mix the solution evenly. Add 7.95g (0.0188mol) of CMC to the mixture of γ-polyglutamic acid and ε-polylysine to control the carboxyl group contained in γ-polyglutamic acid: the amino group contained in ε-polylysine : The molar ratio of CMC was 1:0.5:0.4, reacted in ice bath for 30min, then reacted at room temperature for 2h to form a hydrogel. The formed hydrogel was placed in a dialysis bag, dialyzed in deionized water to swelling equilibrium, and then freeze-dried or vacuum-dried to obtain a spongy dressing. The expansio...

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Abstract

The invention discloses a hydrogel based on a gamma-polyglutamic acid and epsilon-polylysine cross-linked polymer. The hydrogel is prepared from crosslinking gamma-polyglutamic acid and epsilon-polylysine, and has a polymer with the following structural units, wherein m is a natural number ranging from 15 to 45, n is a natural number ranging from 3900-17000 and x is a natural number ranging from 5 to 40. The invention further discloses a preparation method of the hydrogel and an application of the hydrogel in preparing medical wound dressing. The hydrogel prepared by the method disclosed by the invention has good biocompatibility and can be widely applied to medical sanitary articles such as wound dressing.

Description

technical field [0001] The present invention relates to a preparation method of a biocompatible hydrogel, specifically to obtain a biocompatible γ-polyglutamic acid and ε-polylysine crosslinked polymer through a chemical crosslinking method. Preparation method of hydrogel. Background technique [0002] The skin is an important organ of the human body. Due to trauma, burns and other reasons, it may cause extensive damage to the skin, and may lead to complications and endanger human life. How to effectively promote wound healing has always been a subject of unremitting exploration in the medical field. The traditional view is that a dry environment should be created for the wound as much as possible to reduce the chance of infection and facilitate wound healing. However, with the deepening of research, it was found that a moist and infection-free environment is the most favorable for the continuous tissue repair process [Wound repair and regeneration, 2009(17): 505-510]. Und...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/26A61L15/42C08J3/24
CPCA61L15/32A61L15/60C08J3/075C08J3/246C08J2377/04C08J9/286C08J2201/026C08J2201/048C08J2201/054C08J2205/022C08J2205/028C08J2207/12A61L15/26C08J2477/04
Inventor 徐虹迟波夏军李莎冯小海
Owner NANJING TECH UNIV
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