Polymer vesicle capable of promoting wound healing, and preparation method and application of polymer vesicle capable of promoting wound healing

A wound healing and polymer technology, applied in the fields of polymer materials and biomedical engineering, can solve problems such as increased antibiotic resistance and threats to human health, and achieve the effects of preventing wound infection, promoting diabetic wound healing, and good stability

Active Publication Date: 2020-06-05
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the infection of diabetic wounds, at present, it is mainly solved by antibiotics, but the excessive use of antibiotics will lead to the continuous improvement of antibiotic resistance, which poses a serious threat to human health. Therefore, it is necessary to develop antibiotics that can inhibit and kill bacteria but will not lead to new drug resistance

Method used

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  • Polymer vesicle capable of promoting wound healing, and preparation method and application of polymer vesicle capable of promoting wound healing
  • Polymer vesicle capable of promoting wound healing, and preparation method and application of polymer vesicle capable of promoting wound healing
  • Polymer vesicle capable of promoting wound healing, and preparation method and application of polymer vesicle capable of promoting wound healing

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preparation example Construction

[0044] Preparation method of polymersomes promoting wound healing:

[0045] The preparation method of the wound healing-promoting polymersome of the present invention comprises the following steps:

[0046] (1) Preparation steps of antimicrobial peptide polymer and hydrogen sulfide donor

[0047] Mix polycaprolactone, lysine NCA monomer and the first type of solvent, and use a vacuum pump to evacuate and stir the reaction at room temperature for 30 to 50 hours, and then undergo dialysis, suction filtration, and vacuum drying to obtain the antibacterial peptide polymer polycaprolactone-block-polylysine;

[0048] Mix S-aroylthiohydroxylamine (SBTHA), p-aldeoxybenzoic acid, trifluoroacetic acid (TFA), 3A molecular sieves and the second type of solvent, and seal the mixture at room temperature for 1 to 5 hours. After suction filtration, rotary evaporation, recrystallization, and suction filtration, a white powder, compound SATO1, was obtained. Mix SATO1, N,N-dimethylformamide a...

Embodiment 1

[0071] This embodiment provides a method for preparing polymersomes that promote wound healing, comprising the following steps:

[0072] (1) Preparation steps of antimicrobial peptide polymer PCL-b-PLys and hydrogen sulfide donor SATO2

[0073] a. Polycaprolactone PCL-NH 2 (0.426g), lysine NCA monomer (0.903g) and 20mL N,N-dimethylformamide (DMF) were mixed, and the vacuum pump was used to stir the reaction for 32 hours at room temperature, followed by dialysis and suction filtration , vacuum drying to obtain the antimicrobial peptide polymer polycaprolactone-block-polylysine, referred to as PCL-b-PLys;

[0074] b. Mix S-aroylthiohydroxylamine (SBTHA) (100mg, 0.650mmol), p-aldehyde benzoic acid (107mg, 0.710mmol), 10 μL trifluoroacetic acid (TFA), 3A molecular sieves and 3 mL dichloromethane (DCM) The phases were mixed, and the mixture was left sealed at room temperature for 3 hours. After suction filtration, rotary evaporation, recrystallization, and suction filtration, a ...

Embodiment 2

[0083] This embodiment provides a method for preparing polymersomes that promote wound healing, comprising the following steps:

[0084] (1) Preparation steps of antimicrobial peptide polymer PCL-b-PLys and hydrogen sulfide donor SATO2

[0085] a. Polycaprolactone PCL-NH 2 (0.426g), lysine NCA monomer (1.43g) and 30mL dimethyl sulfoxide (DMSO) were mixed, and at room temperature using a vacuum pump vacuum stirring reaction for 50 hours, followed by dialysis, suction filtration, vacuum drying , to obtain the antimicrobial peptide polymer polycaprolactone-block-polylysine, referred to as PCL-b-PLys;

[0086]b. Mix S-aroylthiohydroxylamine (SBTHA) (100 mg, 0.650 mmol), p-formylbenzoic acid (148 mg, 0.980 mmol), 15 μL trifluoroacetic acid (TFA), 3A molecular sieves and 5 mL p-xylene, The mixture was left sealed at room temperature for 5 hours. After suction filtration, rotary evaporation, recrystallization, and suction filtration, a white powder, compound SATO1, was obtained. ...

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Abstract

The invention relates to a polymer vesicle capable of promoting wound healing. The polymer vesicle capable of promoting wound healing comprises a vesicle prepared through assembly of antibacterial peptide polymers, and a hydrogen sulphide donor combined to the vesicle, wherein the antibacterial peptide polymer is polycaprolactone-block-poly[lysine-random-(S-aroyl oxime)] and a derivative thereof.A preparation method of the polymer vesicle capable of promoting wound healing comprises the steps of firstly, enabling polycaprolactone and alpha-amino acid N-carboxyanhydride (NCA) to be subjected to a ring-opening polymerization reaction to synthetize the antibacterial peptide polymers, then enabling the hydrogen sulphide donor to be combined with the polymer vesicle, and finally, enabling thepolymers to be self-assembled to obtain the polymer vesicle which concurrently has antibacterial activity and functions of the hydrogen sulphide donor. The polymer vesicle disclosed by the invention has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, and can slowly release hydrogen sulphide for 12 hours in an aqueous solution containing cysteine. The experiment on animals proves that compared with a commercialized wound repair spray, the vesicle disclosed by the invention can notably promote wound healing of a mouse suffering from diabetes.

Description

technical field [0001] The invention belongs to the fields of polymer materials and biomedical engineering, and relates to polymer vesicles and a preparation method and application thereof. Background technique [0002] Polymer self-assembly technology has always been a research hotspot for researchers in various countries. Various nanomaterials can be obtained through self-assembly technology, such as micelles, vesicles, disc-like structures, worm-like structures, and rod-like structures. Among them, polymersomes have a wider range of applications due to their internal cavity. Stimuli-responsive polymersomes refer to polymersomes that can respond to certain external stimuli. These external stimuli include various types such as pH, temperature, light, magnetic field, and redox conditions. When the external stimulus conditions change, the morphology, structure or composition of the stimulus-responsive polymersomes will change accordingly. It is these intelligent responsive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K38/17A61K33/04A61K47/59A61K9/12A61P31/04A61P17/02A61P3/10A61P31/02
CPCA61K9/0014A61K9/12A61K9/1273A61K33/04A61K38/1729A61K47/593A61P3/10A61P17/02A61P31/02A61P31/04A61K2300/00
Inventor 杜建忠廖雨瑶
Owner TONGJI UNIV
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