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Decellularized matrix-source tissue engineering scaffold and preparation method and application thereof

A technology of tissue engineering scaffold and acellular matrix, which is applied in the field of tissue engineering scaffold derived from acellular matrix and its preparation, can solve the problems of poor mechanical strength, single structure, poor biocompatibility, etc., and achieve good mechanical properties, good Effects of biocompatibility and low immunogenicity

Inactive Publication Date: 2016-09-14
广州市美昊生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the acellular matrix material is used as a scaffold, it has a relatively simple structure, poor biocompatibility, difficulty in applying to multiple clinical indications, and limited clinical use.
[0003] Acellular matrix materials derived from xenogeneic tissues have the advantages of wide sources and high similarity in structure to the human body. At present, many products have been marketed and successfully applied in clinical practice. However, acellular matrix materials derived from xenogeneic tissues have immune rejection and poor mechanical strength. , Insufficient structural controllability and other inherent deficiencies

Method used

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  • Decellularized matrix-source tissue engineering scaffold and preparation method and application thereof
  • Decellularized matrix-source tissue engineering scaffold and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0060] An acellular matrix-derived tissue engineering scaffold, such as figure 1 As shown, the biofilm substrate 1 obtained after pretreatment, degreasing, decellularization, cross-linking and fixing, removing antigens and other steps of the animal film material is adhered to the surface of the biofilm substrate 1 by animal tendon tissue Collagen loose layer 2 obtained by drying collagen obtained through pretreatment, degreasing, decellularization, cross-linking fixation, antigen removal, and collagen extraction and purification. Wherein, the biofilm substrate 1 has a rough surface and a smooth surface, and the loose collagen layer adheres to the rough surface.

Embodiment 2

[0062] A cartilage scaffold derived from an acellular matrix, a biofilm substrate 1 obtained after pretreatment, degreasing, decellularization, cross-linking and fixing, and removing antigens from an animal membrane material, on the surface of the biofilm substrate 1 The surface is adhered to the collagen loose layer 2 obtained by drying the collagen obtained from the animal tendon tissue through the steps of pretreatment, degreasing, decellularization, cross-linking and fixing, removing antigens, and collagen extraction and purification. Wherein the collagen loose layer has pores, the porosity is 65-86%, and the pore diameter is 50-500 μm. The collagen loose layer is fixed on the rough surface of the biofilm substrate through physical adsorption. Alternatively, the loose layer of collagen is bonded and fixed on the rough surface of the biofilm substrate through bioprotein glue. Preferably, the thickness of the loose collagen layer is 0.1-2.9 mm. The cartilage scaffold mater...

Embodiment 3

[0064] The preparation method of a kind of acellular matrix source tissue engineering scaffold of the present invention comprises the following steps:

[0065] (1) Pretreatment: Take fresh animal pericardium and tendon tissues of equal quality, wash them, soak them in a broad-spectrum high-efficiency disinfectant solution of bromogeramine for 15 hours, and remove adipose tissue, fibers and villi;

[0066] (2) Degreasing: use acetone solution with a mass concentration of 3.0% to extract animal pericardium and tendon tissue to remove fat and fat-soluble impurities. The extraction time is 24 hours. The quality of acetone solution and animal pericardium and tendon tissue The ratio is 10:1;

[0067] (3) Decellularization: using Triton-100 with a mass concentration of 0.1% in combination with pepsin at a mass concentration of 0.1% (the stated concentration is the final concentration), ultrasonic digestion of animal pericardium and Tendon tissue for 48 hours to completely remove cel...

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Abstract

The invention relates to a decellularized matrix-source tissue engineering scaffold and a preparation method and an application thereof; the decellularized matrix-source tissue engineering scaffold takes a treated animal membrane material as a biological membrane base material, and the surface of the biological membrane base material is attached with a collagen loose layer. The preparation method of the decellularized matrix source tissue engineering scaffold comprises the process steps of degreasing, decellularizing, antigen removal, cross-linking fixation, collagen extraction, collagen compositing and the like. The flexible and smooth animal membrane material is used as the biological membrane base material, and the defects that a pure collagen scaffold has poor mechanical properties and too fast degradation rate are overcome; with cooperation of the collagen loose layer, a good microenvironment is provided for tissue regenerative repair and cell growth; after being implanted into a body, the decellularized matrix-source tissue engineering scaffold material is gradually degraded along with repairing of defect tissues, also has controllable degradation time, does not exist as a permanent foreign matter, and has no any residual toxicity.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to an acellular matrix-derived tissue engineering scaffold and its preparation method and application. Background technique [0002] With the development of tissue engineering and regenerative medicine, people have carried out a lot of research on the scaffold materials required by tissue engineering technology, including polymer materials, metal materials, bioceramics, heterogeneous materials, etc., but these materials exist in clinical applications. Certain complications or adverse reactions. Therefore, in recent years, acellular matrix-derived biomaterials have received a lot of attention and research, and the research on acellular matrix-derived biomaterials as tissue engineering scaffolds is emerging. The main research content is the repair of human soft tissue injuries by acellular matrix-derived materials. Effect and method of use; such as the treatment of skin bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/44A61L27/50A61L27/58
CPCA61L27/3604A61L27/227A61L27/3687A61L27/50A61L27/58A61L2400/18A61L2430/06A61L2430/34
Inventor 张伟王志杰唐容徐斌
Owner 广州市美昊生物科技有限公司
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