Reg4 protein and application thereof in resisting salmonella enteritidis infection

A Salmonella typhi protein technology, applied in Reg4 protein and its application in resisting Salmonella enteritidis infection, can solve the problems of immature immune system development in children, unfavorable growth and development of children, and high antibiotic residues, and achieve resistance to protease degradation, Strong antibacterial activity and small molecular weight effect

Pending Publication Date: 2021-08-27
SHANGHAI INST OF PEDIATRIC RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ciprofloxacin is one of the main drugs for the clinical treatment of Salmonella infection, but there are already a large number of Salmonella resistant to ciprofloxacin
Moreover, there is a correlation between antibiotic residues and age. Due to the immature development of children's immune system and weak immune function, antibiotic residues in children are high, and there are many types of residual antibiotics, which are very unfavorable to children's growth and development.

Method used

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  • Reg4 protein and application thereof in resisting salmonella enteritidis infection
  • Reg4 protein and application thereof in resisting salmonella enteritidis infection
  • Reg4 protein and application thereof in resisting salmonella enteritidis infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0068] 1. Reg4 protein expression and purification

[0069] 1.1 Main reagents

[0070] (1) pET-28 vector: CV205, Shanghai Jikai Gene Chemical Technology Co., Ltd.

[0071] (2) BL21(DE3) chemically competent cells: C504-03, Nanjing Novozyme Biotechnology Co., Ltd.

[0072] (3) Kanamycin: E004000-5G, Shanghai Yuanxiang Medical Devices Co., Ltd.

[0073] (4) Isopropyl-β-D-thiogalactoside (IPTG) / Isopropylβ-D-thiogalctoside: MB3026, Shanghai Yuanzhi Biotechnology Co., Ltd.

[0074] (5) NUPAGE 4X LDS SAMPLE BUFFER: NP0008, Yingwei Jieji (Shanghai) Trading Co., Ltd.

[0075] (6)Ni-NTA Fast Start Kit(6):30600, Shanghai Yirui Biotechnology Co., Ltd.

[0076] (7) Vivaspin 20,3kDa MWCO PES: 28932358, Shanghai Bergen Biotechnology Co., Ltd.

[0077] (8) PD MidiTrap G-25: 28918008, Shanghai Bergen Biotechnology Co., Ltd.

[0078] (9) Yazyme PS111 Coomassie Brilliant Blue Rapid Staining Solution (Decolorization Free): PS111, Shanghai Yichen Biotechnology Co., Ltd.

[0079] (10) PIERCE ...

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Abstract

The invention provides an application of an active factor, which is characterized in that the active factor is Reg4 or a substance with an amino acid sequence similar to that of Reg4 by about three amino acid sequences; the application comprises at least one of the following applications: A, preparing a medicine for treating and / or relieving diseases caused by salmonella typhimurium infection; and B, preparing a medicine for treating and / or relieving complications caused by salmonella typhimurium infection.

Description

technical field [0001] The present invention relates to an active factor, in particular to Reg4 protein and its application in resisting Salmonella enteritidis infection. Background technique [0002] Salmonella is an important zoonotic infectious pathogen, and its infection is the main factor causing gastrointestinal diseases worldwide. Clinically, Salmonella typhi (Salmonella typhimurium) and Salmonella paratyphi are the main species. Salmonella is the most important pathogen causing bacterial infectious diarrhea in children. Literature reports show that the positive rate of Salmonella infection in stool pathogenic detection of children with diarrhea is as high as 5.5%. Salmonella infection can easily lead to general discomfort symptoms such as fever, vomiting and diarrhea in children, which seriously affects the physical and mental health of children. Salmonella infection is contagious and may be epidemic-like. The use of antibiotics is an important means of treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P31/04A61P1/00C12N15/70C07K14/47C07K1/22C07K1/34C07K1/36
CPCA61K38/1709A61P31/04A61P1/00C12N15/70C07K14/47Y02A50/30
Inventor 肖永陶王伟鹏蔡威
Owner SHANGHAI INST OF PEDIATRIC RES
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