42results about How to "Obvious growth advantage" patented technology

The invention relates to a tobacco culturing method applying large holes for concentrated fertilization. The method is applicable to plain areas of Henan province, and mainly comprises the steps that a base fertilizer is not applied in a culturing process; according to the planting density (16500-18000 plants / hm<2>), the large holes are dug at central positions of tobacco plants planted on a tobacco ridge in a tobacco seedling transplanting process; and various fertilizers are mixed uniformly, applied in the holes according to the calculated fertilization amount of each plant, and sealed after being mixed with soil in the holes sufficiently and uniformly. According to the method, soil water and fertilizer nutrition absorption of the tobacco plants and coordination of water, the fertilizers, air and heat are facilitated; a supply of mineral elements such as N, P,K and Cl is optimized; root development and thickening of the tobacco plants can be facilitated; the disease-resistant ability of tobaccos is improved; the growth advantage of a whole growth period is obvious; resource waste and environmental pollution due to blind fertilization are avoided; the production cost is lowered; effects of oil increasing, aroma enhancing, potassium increasing, alkali reducing, chlorine reducing and starch reducing are exerted in an aspect of improving the quality of tobacco leaves; the internal quality of the tobacco leaves is improved; and economic indicators of the tobacco leaves are increased remarkably.

The invention discloses a quick propagation method for a cinnamomum camphora tree. The quick propagation method comprises the following steps of (1) mixing 60Kg of water into 3.5-7g / mou of gibberellin-heteroauxin-brassinolide and 250-500g / mou of monopotassium phosphate 7-10 days before scion cutting, and spraying to leaf surfaces; in the second day, mixing 30-40Kg of water into 2-3g / mou of uranium nutrison, and spraying; (2) cutting the scion, wherein the scion has the length of 1-2cm, and is provided with at least one leaf; soaking in a rooting culture medium; (3) performing cuttage 1-2 hours after soaking, and using medium-coarse sand or garden perlite as a substrate; (4) controlling the ground temperature as 28-33 DEG C, the greenhouse temperature as 25-28 DEG C, and the humidity as 70-90%, shielding the sun with the light brightness of 90%, rooting after culturing for 20-22 days, budding after culturing for 25-30 days, and growing a finished seedling after culturing for 35-40 days. The method has the advantages that the growth speed is high, the propagation rate is high, the survival rate is high, the cost is low, the subsequent growth is good, the continuity is high, and the like.

The invention discloses a paddy rice NRT 1.1A gene and an application of encoded protein of the paddy rice NRT 1.1A gene to improve the plant yield in breeding. Concretely, protein or an encoded genethereof is applied to regulate the growth and development of a plant. The growth and development is reflected as the single plant yield and / or the strain height and / or grain number and / or flowering time and / or bolting time and / or size of a rosette leaf. The protein is any one of (1) protein having the amino acid sequence shown as in the sequence 1 in a sequence list; and (2) protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in and / or from and / or to the amino acid sequence in the sequence 1 in a sequence list, and is relatedto control of the growth and development of a plant. The paddy rice NRT 1.1A gene and encoded protein are of great importance in breeding of new high-yield paddy rice variety.

The invention discloses a method for producing seeds of supermale Nero-Aurea tilapia, which belongs to the technical field of breeding tilapia. A technical proposal of the method has technical key point of mating WZ female-ZZ male type physiological female oreochromis aurea (ZZ delta female) with XX female-XY male type Nero supermale tilapia (YY male) so as to produce Nero-Austrian genetic supermale tilapia (YZ male). The super tilapia bred by the method has great significance for healthily breeding, improving male rate (100 percent) and promoting tilapia culture technology.

The invention discloses a method for utilizing test tube potatoes for efficiently producing detoxication breeder's seeds.The method comprises the following steps of green net house preparation, test tube potato germination acceleration, matrix preparation, cultivation frame preparation, environmental disinfection, sowing, management after planting and breeder's seed harvesting and storing, wherein the test tube potatoes whose dormancy is not broken are soaked with a gibberellin solution for germination acceleration; a matrix is formed by mixing vermiculite and mushroom dreg, and potassium sulfate compound fertilizer is applied to the matrix; sowing is carried out according to the amount of 250 particles / m<2>, the planting distance is 5 cm, and the line distance is 8 cm; management after planting comprises temperature management, water management, matrix cultivation, fertilization management and disease and pest preventing management; after being harvested, the breeder's seeds are sorted and packaged into detoxification seed potato net bags and stored at the temperature ranging from 2 DEG C to 4 DEG C.The method has the advantages of being high in yield, high in economic benefit, storable and convenient to transport, secondary infection of pathogenic bacteria is eradicated, the potatoes can be planted mechanically, convenience is brought to cultivation and management, the survival rate is high, and the method has great significance in demonstration and popularization of production scales of detoxication breeder's seeds.

The invention relates to a preparation method of placental-chorionic-plate-tissue-derived mesenchymal stem cells, and belongs to the technical field of cell biology. The preparation method of the placental-chorionic-plate-tissue-derived mesenchymal stem cells comprises the following steps: carrying out tissue separation, namely to obtain a placenta tissue sample, separate out chorion tissue, and carry out washing with a tissue cleaning solution until the chorion tissue is semi-translucent; carrying out tissue cryopreservation, namely to put the washed chorion tissue into a cryopreservation solution, and carry out cooling and cryopreservation according to predetermined procedures; carrying out tissue treatment, namely to take out the chorion tissue after the cryopreservation, soak the chorion tissue with ethanol, remove impurities, and perform shearing so as to obtain tissue masses; and then, carrying out cell culture, namely to put the tissue masses into a culture flask, add a mesenchymal stem cell selection medium so as to obtain a mixture, put the mixture into a CO(2) incubator so as to carry out incubation until cell fusion degree reaches 80+ / -10%, carry out cell passage, and continue amplification culture and / or cryopreservation of the passage cells. The preparation method of the placental-chorionic-plate-tissue-derived mesenchymal stem cells enables long-term storage of placental chorionic plate tissue and mesenchymal stem cells separated from the placental-chorionic plate tissue in liquid nitrogen; and moreover, the placental chorionic plate tissue and the mesenchymalstem cells still have maintained cell activity after reconstruction.

The invention provides a method for quickly breaking dormancy of birch-leaf pear seeds with hydroxylamine chloride, which relates to the breaking of seed dormancy and seed germination and belongs to the technical field of plant production. The method comprises the following steps: peeling off pericarp of a ripening fruit; rubbing the peeled fruit to eliminate pulp; soaking seeds in sodium hypochlorite (NaClO) of which the mass volume ratio is 20 percent to remove the pulp adhered to seed capsules; sterilizing the seeds by soaking the seeds in a mercuric chloride solution of which the mass volume ratio is 0.1 percent for 8 minutes; taking the cleaned birch-leaf pear seeds, and presoaking the seeds for 24 hours respectively in the hydroxylamine chloride of which the mass volume ratio is 0.8 percent at room temperature; and sowing the seeds after washing the seeds for 5 to 6 times. The main basis of the method is that the birch-leaf pear seeds have the characteristic of embryo dormancy, the dormancy of the birch-leaf pear seeds can be broken after the treatment with the hydroxylamine chloride, and the method can promote the seed germination, increase the seed germination rate, and has tidy germination.

The invention discloses a method for breeding 'blue spot' hybrids. In the invention, hybrid 'blue spot' fry is produced by selecting single female epinephelus coioides as a female parent and single male epinephelus lanceolatus as a male parent and by distant hybridization, artificial parturition expediting, insemination, incubation and fry breeding. The method for breeding the 'blue spot' hybrids of the invention is simple in operation and suitable for mass production; the bred 'blue spot' fry has a survival rate of 5 to 10 percent and a breeding survival rate of over 95 percent, and both the survival rates are higher than those of the common groupers, so that the bred 'blue spot' fry has a promising market prospect.

The invention discloses a method for producing biological organic fertilizer by utilizing a pruned branch and an obsolete fruit tree of an apple tree. The method particularly comprises the following steps of a step 1, pretreatment of raw materials: crushing the apple tree and the pruned branch thereof into sawdust units, screening a sawdust raw material with the length of 20mm to 60mm, carrying out high-temperature and high-pressure steam pretreatment on the sawdust raw material, and naturally drying for 4h to 6h after the treatment is completed; a step 2, preparing a fermentation microorganism mixed microbial inoculum; a step 3, carrying out composting fermentation on the raw material subjected to the pretreatment in the step 1; a step 4, repeatedly pile-turning fertilizer fermented in the step 3, and carrying out the composting fermentation again; a step 5, packaging and storing the fertilizer. The method is used for effectively returning agriculture-forestry waste to a field and solving the pollution problems caused to an environment and soil in the stacking and incineration processes of the pruned branch and the obsolete fruit tree of the apple tree.

The invention discloses a large scale producing method for all female mandarin fishes. The method includes the following steps: S1, the breeding processing for sex reversal of mandarin fishes: sex reversal of mandarin fishes is performed by adopting the method that bait fishes which are fed with forage mixed with 17alpha-methyl testosterone hormone are ingested by the mandarin fishes in ten days after the mandarin fish fries start feeding and before the differentiation of the sexual gland; S2, the sexual gland identification for the sex reversal individuals; S3, the test cross for the sex reversal individuals; S4, the production for all female mandarin fish fries. The sex reversal of mandarin fishes is performed by adopting the method that the bait fish which are fed with forage mixed with17alpha-methyl testosterone hormone are ingested by the mandarin fishes. The technology of inducing sex reversal of mandarin fishes with high efficiency is established to cooperate with the corresponding biological sex sexual gland microscopic identification technology and the genetic sex test cross identification technology to provide technical support for the control of the mandarin fish sex and the all-female breeding. The sex reversal of the masculinization of 99% of female fishes of mandarin fishes can be realized by the provided method, the female proportion of all-female mandarin fishproduction can reach 100%, and all-female mandarin fishes have obvious growth advantages compared with common breeding mandarin fishes so that the method has an excellent application prospect.

The invention discloses a peony high-branch grafting method. The method comprises the steps of engrafting bud selection, stock selection, bud removal operation, bud grafting operation, survival observation, post-grafting management within the same year and pre-bloom management in the next year. By means of the grafting method, the survival rate is high, a grafted peony is luxuriant in foliage and orderly and attractive in appearance and shape, the flower diameter is obviously larger than that of a plant of an original variety, the expectation that various peony flowers can grow out of the same peony plant is achieved, the flower colors are diversified, the flower types are rich, and the ornamental value is greatly improved.

The invention provides a current-year aquaculture method of hybrid pelteobagrus fulvidraco. The aims of current-year fry breeding, current-year aquaculture and current-year benefiting can be achievedby a series of technical processes such as fry breeding, reasonable stocking, domesticated feeding, water quality management, daily management, disease control and efficient fishing. Known from a contrast experiment, the growth performance of the hybrid pelteobagrus fulvidraco is obviously superior to that of common pelteobagrus fulvidraco, the hybrid pelteobagrus fulvidraco propagated in the current year can grow to the weight of 50-150 g, the aquaculture period of the pelteobagrus fulvidraco is greatly shortened, and the current-year aquaculture method is an efficient aquaculture technical method of hybrid pelteobagrus fulvidraco.

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

The invention relates to an artificial domesticating and planting method of wild Paris polyphylla. The method includes steps: land selection and preparation, seed selection, seed seedling raising, field management and harvesting, wherein field management includes steps: seedling supplementing, intertillage weeding, fertilizer-water management, flower removal, earthing up and shading. Paris polyphylla domesticated and cultivated by adopting the method is normal in growing and free of pest and disease; chemical fertilizer and pesticide are not used in the whole cultivation process, so that root blocks of underground portions of plants are thick and strong, branch leaves of overground portions of the plants are green and beautiful, and the plants are high in resistance to disease and pest, high in yield and environment-friendly. Actual measurement of several years shows that saponin content in active ingredients is up to 2.27 metering units, which is 2-3 times of Standards of Pharmacopoeia. No toxic and harmful heavy metal and residual pesticide are contained, so that environment protection is realized, and a foundation is laid for producing healthy and safe Chinese medicinal materials for people.

The invention discloses a north China black head mutton sheep crossbreed method, and belongs to the technical field of animal breeding. The north China black head mutton sheep crossbreed method includes: selecting small tailed cold sheep as female parents and Dorper sheep as male parents; performing grading hybridization; performing crossing and consolidating, selecting and domestication; and selecting black head sheep having optimal indexes as a new strain blood relationship. The hybrid north China black head mutton sheep are high in benefits, can achieve triple pregnancy during two years, can deliver 2-3 sheep each time, and has the survival rate of 95%; the feed conversion rate is high, the feed threshold is low, the growth vigor is significant, the fatness of the sheep is good, and the north China black head mutton sheep has excellent virus resistance.

The invention, which relates to the technical field of crop cultivation, provides a pepper cultivation management method. The method comprises the following steps that: step one, seeds are processed;step two, strong seedling cultivation is carried out; to be specific, prepared nutrient soil is disinfected and then the disinfected soil is placed in a nutrient bowl, a paper bag or a plate for seedling cultivation; seedling separation is carried out properly during the cultivation process, so that plant diseases and insect pests are prevented; if any seedling becomes sick and weak, the seedlingis pulled out timely and winter protection and heat preservation are also carried out; step three, field planting is carried out; step four, management after field planting is carried out; to be specific, management before and after recovery and management after result obtaining are carried out; step five, prevention of diseases and insect pests is carried out; and step six, harvesting is carriedout; to be specific, fresh peppers are harvested and then mature peppers are harvested. With the pepper cultivation management method provided by the invention, the pepper yield and quality can be improved effectively.

The invention discloses a method for quickly forming a citrus orchard, and belongs to the technical field of fruit tree cultivation. The method comprises the following steps: 1, strong seedling selection; 2, mechanical garden building; 3, rhizosphere soil improvement; 4, fertilizer and water management; 5, shaping without pruning; 6, effective control for pest and disease damage; and 7, tree disk covering. According to the method, basic conditions are created for rapid expansion of root systems in a mode of preferably selecting strong seedlings and locally improving soil in a root zone, nutrient supply in the citrus seedling stage is met, and the tedious procedure of applying base fertilizer in autumn in the seedling stage is reduced; a garden building mode of deep ditch, wide compartment and single-row ridging facilitates mechanical light operation, and the later-stage management and protection cost is greatly reduced; by application of the technology of shaping without pruning, rapid outward expansion of plant crowns is realized, and nutrient waste caused by pruning is avoided; and high-efficiency management in the seedling stage is achieved through pest and disease key period control and formulated fertilization technologies, the dosage of chemical fertilizers and pesticides and input of labor force are remarkably reduced, the plant growth vigor and the fruiting period are 2-3 years earlier than those in the prior art, and early fruiting, high yield and stable yield of fruit trees and sustainable production of soil are achieved.

The invention discloses a rapid breeding method of a new fish strain. The method comprises the following steps: a, selecting parent fish; b, labeling the parent; c, constructing a family; d, cultivating the family; e, determining dominant parent combination; and f, constructing a new strain. Through adopting the technical scheme, the rapid breeding method of the new fish strain can be used for breeding the new strain with relatively obvious growth domination in a short time. The technology is suitable for fishes having long sexual maturity time, such as four major Chinese carps, sturgeons and the like.

The invention discloses a breeding method of a lake culture strain, and belongs to the technical field of lake culture. The breeding method includes the steps: parent fish selection; breeding population selection; first-generation breeding; second-generation breeding; third-generation breeding; new strain determining and the like. According to the method, a new strain with relatively obvious growth advantages can be bred within a short time, and the method is applicable to four major Chinese carps, acipenseridae fishes and the like with long sexual maturity time. According to the method, breeding populations are selected before breeding, disadvantaged populations can be effectively removed, so that later breeding efficiency is effectively improved, diversity of biological genes at a cultivation stage is enriched to some extent, close breeding probability is reduced, the quality of finally cultured strains is improved, a water environment regulator is used, dissolved oxygen and nutrientelements in water can be increased, microorganism environments in the water are improved, so that the physique of bighead carps is improved, and the survival rate of the bighead carps is increased.

The invention belongs to the technical field of algological biology. In view of defects in the prior art, the invention provides an algae filament resuscitation fluid for shellfish feeding microalgae,which is characterized by comprising the following components in parts by weight: 500-1000 parts of potassium nitrate, 500-1000 parts of potassium dihydrogen phosphate, 200-300 parts of ethylene diamine tetraacetic acid, 100-200 parts of ferric trichloride, 100-200 parts of sodium bicarbonate, 10-20 parts of vitamin B1, 10-20 parts of vitamin B12, 2-5 parts of trace elements and 1500-2000 parts of distilled water. The algae filament resuscitation fluid for the shellfish feeding microalgae provided by the invention is safe and reliable, has no adverse effect on the growth of the shellfish feeding microalgae, avoids the waste of a culture solution caused by algae pollution, and effectively reduces the production cost of the shellfish seedling industry.

The invention relates to an acclimatizing and cultivating method for medicinal wild paris polyphylla. The method comprises the steps: carrying out seed selection: selecting paris polyphylla plants, with the following features, as seed plants from a wild paris polyphylla growing forest, wherein the features are as follows: stems are straight, thick and strong, the color of stem bodies is pink green, the middle-upper part of each leaf is slightly slender, the middle-lower part of each leaf is slightly wide, the leaf surface color is pink green, petals are slender, tips of the petals are tapered, stamens are slightly wide, the middle of each stamen has a slender granular structure, pollen is rich, pistils are reddish-brown, petal bodies are wide, tips of the petal bodies are slightly hooked inwards, fruits are high in growth speed and early in ripening, fruit bodies are clear in edges and corners, fruit peels are thin, each fruit has a red circular scar, grains are red, the seed setting rate is high, the grains are full and sturdy, seed kernels are white and circular, and spores are obvious; carrying out seedling raising: carrying out seedling raising by using seeds of the selected seed plants, peeling red seeds, putting the peeled red seeds underground by 1cm to 1.5cm, and carrying out moisture, temperature and humidity management until young seedlings emerge; carrying out field transplanting after the young seedlings grow up; covering soil surrounding the plants with green wormwood branches after the transplanted seedlings survive; and carrying out harvesting after the plants continuously grow for 3 to 7 years. The paris polyphylla acclimatized and cultivated by the method is normal in growth, high in yield and high in drug effects, is free of insect pests and diseases and is green and environmentally friendly.

The invention provides a method for preparing hybrids of bream and triangular bream. The method includes parent selection, intensive cultivation, artificial induction of labor, artificial insemination, hatching and cultivation of seedlings, and other technical links. The invention has strong operability. The obtained hybrid has a beautiful body and obvious growth advantages, and has a mild temperament, does not like jumping, has strong stress resistance, is easy to catch, and has a high survival rate for long-distance transportation of fish species and adults; The degree of tenderness is better than that of triangular bream, and the content of umami amino acids is higher; it has a wider range of feeding habits, better resistance to hypoxia and stress; the hybrid is sexually fertile, providing a source for further tapping the breeding potential of hybrid offspring It is possible to breed new breeds with excellent traits and stable genetics.

The invention discloses a method for breeding 'blue spot' hybrids. In the invention, hybrid 'blue spot' fry is produced by selecting single female epinephelus coioides as a female parent and single male epinephelus lanceolatus as a male parent and by distant hybridization, artificial parturition expediting, insemination, incubation and fry breeding. The method for breeding the 'blue spot' hybrids of the invention is simple in operation and suitable for mass production; the bred 'blue spot' fry has a survival rate of 5 to 10 percent and a breeding survival rate of over 95 percent, and both the survival rates are higher than those of the common groupers, so that the bred 'blue spot' fry has a promising market prospect.

The invention relates to a tobacco culturing method applying large holes for concentrated fertilization. The method is applicable to plain areas of Henan province, and mainly comprises the steps that a base fertilizer is not applied in a culturing process; according to the planting density (16500-18000 plants / hm<2>), the large holes are dug at central positions of tobacco plants planted on a tobacco ridge in a tobacco seedling transplanting process; and various fertilizers are mixed uniformly, applied in the holes according to the calculated fertilization amount of each plant, and sealed after being mixed with soil in the holes sufficiently and uniformly. According to the method, soil water and fertilizer nutrition absorption of the tobacco plants and coordination of water, the fertilizers, air and heat are facilitated; a supply of mineral elements such as N, P,K and Cl is optimized; root development and thickening of the tobacco plants can be facilitated; the disease-resistant ability of tobaccos is improved; the growth advantage of a whole growth period is obvious; resource waste and environmental pollution due to blind fertilization are avoided; the production cost is lowered; effects of oil increasing, aroma enhancing, potassium increasing, alkali reducing, chlorine reducing and starch reducing are exerted in an aspect of improving the quality of tobacco leaves; the internal quality of the tobacco leaves is improved; and economic indicators of the tobacco leaves are increased remarkably.

The present invention discloses a high cephalosporin C yield bacterial breeding method, and relates to the field of microorganism bacterial breeding. According to the present invention, a metabolic regulation breeding method is adopted to overcome a saturation effect of bacterial on a mutagen so as to easily achieve mutation bacterial generation; a semi-continuous fermentation method is adopted to make bacterial with excellent characteristics rapidly establish growth advantages so as to increase chances of screening of excellent bacterial; and the bacterial breeding method is simple, fast and efficient.

The invention provides a Si-Y alloy preparation and alloy structure control method for reactive penetration. During the solidification process, the solidification rate can be changed so as to meet different microscopic structure requirements; the temperature gradient can be kept at 150-240 K / cm; and along with the change of the solidification rate from 0.1 mm / min to 3.0 mm / min, uniform full strip-shaped structures, transition structures for evolution from the strip shape to the island shape, and uniform full island-shaped structures can be acquired separately. A smelting parameter range acquired through the Si-Y alloy preparation and alloy structure control method is not limited to a directional solidification smelting method, and can be applied to an electric resistance smelting method, an induction smelting method, a laser smelting method and the like; through the combination with the own solidification rate regulation situations, various processes can be regulated within a solidification rate range proposed by the Si-Y alloy preparation and alloy structure control method; and different morphologies of uniform full eutectic structures without the primary Si phase can be acquired.

The invention discloses a crossbreeding method of hypoxia-resistant epinephelus awoara. The method comprises the following steps: 1) parent fish cultivation of epinephelus tukula and acquisition of sperms; 2) sperm cryopreservation; 3) parent fish cultivation of epinephelus awoara and ovum acquisition; 4) artificial insemination: frozen seminal fluid of the epinephelus tukula is taken, after beingunfrozen, the seminal fluid is uniformly mixed with ova, and seawater is added for fertilization; standing is performed, high-salinity seawater is added, and floating fertilized eggs are collected; (5) hatching management: hatching is conducted for 6-10 hours, the fertilized eggs are added into the high-salinity seawater, the floating fertilized eggs are collected again and put into a culture pond for hatching and culturing; and (6) later-period cultivation: biological baits are utilized for cultivation to obtain epinephelus awoara hybrid fries. According to the crossbreeding method, the fries obtained through crossbreeding of the epinephelus awoara is high in survival rate, high in growth speed, resistant to low oxygen and low temperature and uniform in filial generation form and body color, the chromosome karyotype is 2n=1sm+47t, and the epinephelus awoara has unique hereditary features and remarkable hybridization advantages, and is high in market acceptance degree and considerablein economic benefit.

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

The present invention finds that the glucose inhibitory effect in the heat-resistant yeast with hexokinase KmHXK knockout is eliminated to a large extent, realizing the co-utilization of various sugars and glucose, and overexpressing glucokinase KmGLK can restore the glucose metabolism after KmHXK knockout In the present invention, it is found that the bacterium still maintains the characteristics of releasing the glucose inhibitory effect, and can be used as a platform strain; the present invention successfully obtains a heat-resistant yeast strain YHY013 that can efficiently co-utilize glucose and xylose to produce xylitol with this platform strain. The deposit number is CGMCC No.15347. The strain can effectively utilize glucose and xylose in natural fermentation substrates such as xylose mother liquor or corncob hydrolyzate to ferment xylitol under the condition of 42°C, and the yield of xylitol produced by using corncob hydrolyzate exceeds Other strains have been reported.

The invention relates to AOP premix, soft-shelled turtle feed containing the AOP premix, a preparation method and an application. The AOP premix comprises, by weight, 39.6-50 parts of sodium alga acid, 0.1-0.3 part of chitosan, 5-15 parts of lactobacillus acidophilus, 5-15 parts of magnesium phosphate, 8-10 parts of sodium acetate, 0.5-1.5 parts of potassium sorbate, 10-20 parts of decanoylacetaldehyde, 2.5-7.5 parts of lauryl aldehyde and 2.5-7.5 parts of disease-resistant growth promoting agent. The soft-shelled turtle feed comprises, by weight, 69.2-77 parts of animal source protein matter, 15-21 parts of bean pulp, 1-3 parts of sesame seed meal, 1-3 parts of vital gluten, 1.8-2 parts of unhulled rice buds, 1-3 parts of vegetable oil and 0.8-1.2 parts of the AOP premix. The AOP premix has the good disease-resistant growth promoting effect and can enhance the fish body immune function, remarkably increase the survival rate and yield of soft-shelled turtles and remarkably improve the quality of the soft-shelled turtles. The soft-shelled turtle feed is proper in nutrition matching, the preparation method is easy and practicable, cost is low, food calling performance is high, palatability is good, absorption is quick, and digestion is easy.