Tissue culture and rapid propagation method of micro tubers of rhizoma bletillae

Abstract

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

Description

technical field [0001] The invention relates to a method for tissue culture of bletilla and particularly relates to a method for tissue culture and rapid propagation of bletilla microtubers. Background technique [0002] Bletilla striat is a plant of the genus Bletilla Rchb.f. in the family Orchidaceae. It is a perennial herb with pseudobulbs in the basement. , After 1977, all editions of "Chinese Pharmacopoeia" are recorded. Its taste is bitter, sweet, and astringent; slightly cold in nature, it returns to the lung, liver, and stomach meridians, and has the functions of astringent, hemostasis, swelling and granulation. It is mainly used to treat hemoptysis, hematemesis, traumatic bleeding, sore swelling, chapped skin, tuberculosis hemoptysis, ulcer bleeding, etc. Modern research has found that the root tuber contains nearly 50 kinds of compounds, which has broad application prospects in medicine, chemical industry, food, etc., and has great potential for comprehensive uti...

AI Technical Summary

Problems solved by technology

The seedling cultivation methods and cultivation methods of the above groups are very easy to differentiate into clustered seedlings. The seedlings are slender and thin, grow slowly, and it is difficult to form micro-tubers. There are many problems such as low rate, low multiplication rate, and low tuber yield after planting, and the industrialized production of seedlings has not yet been realized.

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