Production method of low-salt oyster polypeptide and oligosaccharide nutrition powder
A production method and technology for oyster polypeptides, which are applied in the directions of fish protein composition, food hydrolysis, food science, etc., can solve the problems of underutilized value of oysters, difficult to achieve ideal effects, decreased protease activity, etc., to prevent The effect of serious sticking to the wall, shortening the cooling time and reducing energy consumption
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Embodiment 1
[0043] Embodiment 1: the production of low-salt oyster polypeptide and oligosaccharide nutrition powder
[0044] (1) Desalination: Take 1 kg of shelled oyster meat, add 4 L of ice water to soak for 45 minutes at 4 °C, drain the water, then add 4 L of ice water to soak for 45 min at 4 °C, repeat 3 times.
[0045] (2) Sterilization of samples and protein denaturation: Homogenize oyster meat after soaking and desalination, homogenize at 5000rpm for 10 minutes, then place in a water bath at 90°C for 10 minutes, and denature proteins while sterilizing samples.
[0046] (3) Sample cooling: Add 2L of distilled water at 25°C to make the temperature of the sample liquid 40.2°C, and dilute the sample liquid at the same time. At this time, the pH of the sample liquid is 6.58;
[0047] (4) High pH protease enzymatic hydrolysis: adjust the pH value to 8.0 with 1mol / L NaOH, place the sample in a water bath set at 45°C, add 8g, 0.8% (w / w) trypsin to hydrolyze the protease for 1.5 h, at th...
Embodiment 2
[0056] Embodiment 2: Production of low-salt oyster polypeptide and oligosaccharide nutritional powder
[0057] (1) Desalination: Take 1 kg of shelled oyster meat, add 6L of ice water, soak at 4°C for 45 minutes, drain the water, add 6L of ice water for 45 minutes, repeat twice.
[0058] (2) Sample sterilization and protein denaturation: Homogenize the soaked and desalted oysters for 10 minutes at 5,000 rpm, then place them in a water bath at 80°C for 15 minutes.
[0059] (3) Sample cooling: after high temperature treatment, add 1.5L of distilled water at 25°C, the temperature of the obtained sample liquid is 46.7°C, and dilute the sample liquid at the same time.
[0060] (4) High pH protease hydrolysis: adjust the pH value to 8.0 with 1mol / L NaOH, place the sample in a water bath set at 50°C, add 10g, 1.0% (w / w) alkaline protease for proteolysis 1h.
[0061] (5) Low pH protease enzymatic hydrolysis: After high pH protease enzymatic hydrolysis, the pH of the sample solution...
Embodiment 3
[0065] Embodiment 3: the production of low-salt oyster polypeptide and oligosaccharide nutrition powder
[0066] (1) Desalting: Take 1 kg of shelled oyster meat, add 2L of ice water, soak at 4°C for 30 minutes, drain the water, then add 4L of ice water and soak at 4°C for 30 minutes, repeat 6 times.
[0067] (2) Sample sterilization and protein denaturation: Homogenize oyster meat after soaking and desalination, homogenize at 5000 rpm for 10 minutes, then place in a water bath at 100°C for 10 minutes, and denature the protein while sterilizing the sample.
[0068] (3) Sample cooling: Add 2L of distilled water at 28°C to make the temperature of the sample liquid at 55°C, and dilute the sample liquid at the same time. At this time, the pH of the sample liquid is 6.58.
[0069] (4) High pH protease hydrolysis: adjust the pH value to 8.0 with 1mol / L NaOH, place the sample in a water bath set at 55°C, add 8g, 0.8% (w / w) trypsin to hydrolyze the protease for 1.5 h; at this time, ...
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