Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production method of low-salt oyster polypeptide and oligosaccharide nutrition powder

A production method and technology for oyster polypeptides, which are applied in the directions of fish protein composition, food hydrolysis, food science, etc., can solve the problems of underutilized value of oysters, difficult to achieve ideal effects, decreased protease activity, etc., to prevent The effect of serious sticking to the wall, shortening the cooling time and reducing energy consumption

Active Publication Date: 2016-02-17
荣成市海洋绿洲生物科技有限公司
View PDF10 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) Oysters that grow in the ocean contain a certain amount of salt, and are prepared into polypeptide products without treatment. The salt content exceeds the acceptable range for general use, resulting in certain restrictions on the use of the obtained products
The use of membrane desalination will not only increase the cost, but also complicate the process
[0006] (2) Due to the growth environment and other reasons, oysters often contain certain bacteria, and heat treatment in the later stage of enzymatic hydrolysis can achieve a certain sterilization effect, but it is difficult to achieve the desired effect, which has a certain impact on the quality of the product
[0007] (3) Most studies mainly use a single protease for enzymatic hydrolysis of proteins, and proteases generally have certain enzymatic cleavage sites, which have certain restrictions on the degradation of substrates. When compound enzymes are used for simultaneous enzymatic hydrolysis, May cause several proteases to degrade each other and reduce protease activity
[0008] (4) Most of these patents use oyster powder as raw material instead of directly using fresh oysters. The purpose of the research is only to use the protein component or single polysaccharide component in oysters, and the value of oysters has not been fully utilized

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of low-salt oyster polypeptide and oligosaccharide nutrition powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: the production of low-salt oyster polypeptide and oligosaccharide nutrition powder

[0044] (1) Desalination: Take 1 kg of shelled oyster meat, add 4 L of ice water to soak for 45 minutes at 4 °C, drain the water, then add 4 L of ice water to soak for 45 min at 4 °C, repeat 3 times.

[0045] (2) Sterilization of samples and protein denaturation: Homogenize oyster meat after soaking and desalination, homogenize at 5000rpm for 10 minutes, then place in a water bath at 90°C for 10 minutes, and denature proteins while sterilizing samples.

[0046] (3) Sample cooling: Add 2L of distilled water at 25°C to make the temperature of the sample liquid 40.2°C, and dilute the sample liquid at the same time. At this time, the pH of the sample liquid is 6.58;

[0047] (4) High pH protease enzymatic hydrolysis: adjust the pH value to 8.0 with 1mol / L NaOH, place the sample in a water bath set at 45°C, add 8g, 0.8% (w / w) trypsin to hydrolyze the protease for 1.5 h, at th...

Embodiment 2

[0056] Embodiment 2: Production of low-salt oyster polypeptide and oligosaccharide nutritional powder

[0057] (1) Desalination: Take 1 kg of shelled oyster meat, add 6L of ice water, soak at 4°C for 45 minutes, drain the water, add 6L of ice water for 45 minutes, repeat twice.

[0058] (2) Sample sterilization and protein denaturation: Homogenize the soaked and desalted oysters for 10 minutes at 5,000 rpm, then place them in a water bath at 80°C for 15 minutes.

[0059] (3) Sample cooling: after high temperature treatment, add 1.5L of distilled water at 25°C, the temperature of the obtained sample liquid is 46.7°C, and dilute the sample liquid at the same time.

[0060] (4) High pH protease hydrolysis: adjust the pH value to 8.0 with 1mol / L NaOH, place the sample in a water bath set at 50°C, add 10g, 1.0% (w / w) alkaline protease for proteolysis 1h.

[0061] (5) Low pH protease enzymatic hydrolysis: After high pH protease enzymatic hydrolysis, the pH of the sample solution...

Embodiment 3

[0065] Embodiment 3: the production of low-salt oyster polypeptide and oligosaccharide nutrition powder

[0066] (1) Desalting: Take 1 kg of shelled oyster meat, add 2L of ice water, soak at 4°C for 30 minutes, drain the water, then add 4L of ice water and soak at 4°C for 30 minutes, repeat 6 times.

[0067] (2) Sample sterilization and protein denaturation: Homogenize oyster meat after soaking and desalination, homogenize at 5000 rpm for 10 minutes, then place in a water bath at 100°C for 10 minutes, and denature the protein while sterilizing the sample.

[0068] (3) Sample cooling: Add 2L of distilled water at 28°C to make the temperature of the sample liquid at 55°C, and dilute the sample liquid at the same time. At this time, the pH of the sample liquid is 6.58.

[0069] (4) High pH protease hydrolysis: adjust the pH value to 8.0 with 1mol / L NaOH, place the sample in a water bath set at 55°C, add 8g, 0.8% (w / w) trypsin to hydrolyze the protease for 1.5 h; at this time, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a production method of a low-salt oyster polypeptide and oligosaccharide nutrition powder. According to the production method, oyster obtained via shell removing is taken as a raw material, is repeatedly immersed in ice water at 4 to 10 DEG C for 2 to 4h; the oyster obtained via immersion desalting is subjected to slurry homogenizing, and is subjected to high temperature treatment for disinfection and protein denaturation; obtained oyster slurry is cooled with normal temperature water; pH value is adjusted to be 7.0 to 9.0, and 0.5 to 1.0wt% of protease is added for protein enzymatic hydrolysis; after high pH protease enzymatic hydrolysis, the temperature is adjusted, and 0.5 to 1% of a weakly acidic protease is added for enzymatic hydrolysis; amylase is added for degradation of glycogen; an obtained product is subjected to high temperature treatment, filtering, and spray drying so as to obtain the oyster polypeptide and oligosaccharide nutrition powder. The production method is simple; cost is low; and the obtained oyster polypeptide and oligosaccharide nutrition powder can be absorbed by human body easily.

Description

technical field [0001] The invention relates to the field of aquatic product processing, in particular to a method for producing low-salt oyster polypeptide and oligosaccharide nutritional powder. Background technique [0002] Oyster (Oyster, Crassostreagigas ), also known as oysters and oysters, is one of the famous economically cultured shellfish in my country. It is known as "the brown rice of the sea" and "the source of the root" in Japan, and "the milk of the sea" and "the fruit of the sea" in the West. . The protein content in oysters is as high as 45% to 57%, the amino acid content is perfect, and the completeness and quality ratio of essential amino acids are better than human milk and cow milk. The sugar contained in oysters is as high as 20% to 40%, which has anti-fatigue, improves human heart and blood circulation functions, and has liver protection effects. Zinc, calcium, iron, phosphorus and other trace elements, vitamin B in oysters 2 Taurine and taurine are...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L33/00A23L5/00A23J1/04A23L33/17
CPCA23J1/04A23V2002/00A23V2250/543A23V2300/28
Inventor 曹敏杰邱娟蔡秋凤刘光明沈建东章骞
Owner 荣成市海洋绿洲生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products