Alkaline thermal-stability SGNH family esterase EstD1 and gene thereof

A thermostable, family-friendly technology, applied in the field of genetic engineering, which can solve the problems of difficult purification, long fermentation period of natural strains, and difficulty in mass production.
CN104894081AActive Publication Date: 2015-09-09YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2015-09-09

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Abstract

The invention discloses alkaline thermal-stability SGNH family esterase EstD1. The amino acid sequence of the alkaline thermal-stability SGNH family esterase EstD1 is one of the amino acid sequence (1) shown in SEQ ID NO.1 and the amino acid sequence (2), wherein one or more pieces of amino acid are added or replaced or lacked or inserted, shown in SEQ ID NO.1. The invention further discloses a gene EstD1 of the alkaline thermal-stability SGNH family esterase EstD1, the nucleotide sequence of the gene EstD1 is one of the nucleotide sequence (1) shown in SEQ ID NO.2 and the nucleotide sequence (2), wherein one or more pieces of nucleotide are added, replaced, lacked or inserted, shown in SEQ ID NO.2, the nucleotide sequence (3) formed through hybridization with the nucleotide sequence (1) or nucleotide sequence (2) under strict conditions and the nucleotide sequence (4) which is different from the nucleotide sequence (1), the nucleotide sequence (2) and the nucleotide sequence (3) due to the degeneracy of a genetic codon. The alkaline thermal-stability SGNH family esterase EstD1 can be used for deacetylation of 7-ACA.
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Description

technical field

[0001] The invention relates to an alkaline thermostable SGNH family esterase EstD1, its coding gene, and its application in 7-ACA deacetylation, belonging to the technical field of genetic engineering. Background technique

[0002] SGNH family esterase (esterase, EC 3.1.1.1) is a class of hydrolytic enzymes with broad substrate specificity, including lipase, protease, thioesterase, aromatic esterase, lysophospholipase, carbohydrate esterase, acyl transfer enzyme activity. This family was identified around 1995, but its members are still rarely characterized. They differ from most of the α / β hydrolase superfamily esterases that contain the typical conserved sequence of GxSxG, but contain the unique conserved sequence of GDSL , is a new family of esterases. The esterases of the GDSL family contain 5 conservative sequence blocks (five consensus sequence blocks I-V), and 4 relatively conservative catalytic sites Ser, Gly, Asn and His in the 5 conservative sequ...

Claims

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