Rice genetic engineering sterile line breeding method

A genetic engineering and rice technology, applied in the field of genetic engineering genetics and breeding, can solve problems such as unfree mating, delayed programmed death, and sterility

Inactive Publication Date: 2018-06-12
QINGDAO YUANCE GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low
However, the rice EAT1 gene mutation will cause th...

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  • Rice genetic engineering sterile line breeding method
  • Rice genetic engineering sterile line breeding method
  • Rice genetic engineering sterile line breeding method

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Embodiment Construction

[0043] The present invention is described in detail below in conjunction with embodiment. In order to make the object, technical solution and advantages of the present invention clearer and clearer, the present invention will be described in further detail below with reference to the accompanying drawings and examples, but the present invention is not limited to these examples.

[0044] In the invention, the common nuclear sterile gene EAT1 of rice is linked with the lethal gene ZMAA1 of maize, and the red fluorescent protein gene DsRED is used as a reporter gene to genetically modify the rice eat1 mutant to obtain F1 generation hybrids. The hybrids of the F1 generation follow Mendel's segregation law in the process of self-fertilization, and the offspring produced include heterozygotes that can maintain the triple-linkage genes and sterile sterile lines, which are the so-called third-generation sterile lines.

[0045] In order to solve the above-mentioned technical problems, ...

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Abstract

The invention discloses a rice genetic engineering sterile line breeding method. The method includes following steps: A, acquiring a DsRED gene expression box; B, acquiring a rice EAT1 gene expressionbox; C, amplifying corn lethal gene ZMAA1; D, amplifying corn pollen specific promoter Pg47; E, connecting gene expression elements: step 1, connecting a DsRED gene expression element onto a pCAMBIA1300 carrier; step 2, introducing a complete rice EAT1 gene onto the pCAMBIA1300 carrier where the DsRED gene expression element is already introduced in the step 1. F1-generation heterozygote abides by Mendel's law of segregation in the process of self fruiting, and generated offspring include heterozygote capable of maintaining triply linked genes and infertile sterile lines.

Description

technical field [0001] The invention relates to the technical field of genetic engineering genetic breeding, in particular to a method for breeding male sterile lines of rice genetic engineering. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a genetic to...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/66A01H5/00A01H6/46
CPCC12N15/66C12N15/8212C12N15/8231C12N15/8289
Inventor 张国栋刘佳音邹丹丹张佩邵晓宇徐春莹米铁柱
Owner QINGDAO YUANCE GRP CO LTD
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