Rice genetic engineering sterile line breeding method

Abstract

The invention discloses a rice genetic engineering sterile line breeding method. The method includes following steps: A, acquiring a DsRED gene expression box; B, acquiring a rice EAT1 gene expressionbox; C, amplifying corn lethal gene ZMAA1; D, amplifying corn pollen specific promoter Pg47; E, connecting gene expression elements: step 1, connecting a DsRED gene expression element onto a pCAMBIA1300 carrier; step 2, introducing a complete rice EAT1 gene onto the pCAMBIA1300 carrier where the DsRED gene expression element is already introduced in the step 1. F1-generation heterozygote abides by Mendel's law of segregation in the process of self fruiting, and generated offspring include heterozygote capable of maintaining triply linked genes and infertile sterile lines.

Description

technical field [0001] The invention relates to the technical field of genetic engineering genetic breeding, in particular to a method for breeding male sterile lines of rice genetic engineering. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a genetic to...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

However, the rice EAT1 gene mutation will cause the tapetum cells to fail to degrade in time, and the programmed death will be delayed, so the callose enzyme secreted by the tapetum will affect the development and separation of microspores, and finally show sterility

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