Efficient establishment method for Chinese cymbidium sexual polyploid

A polyploid and national orchid technology, applied in horticultural methods, botany equipment and methods, plant genetic improvement, etc., can solve the problems of low polyploid survival rate, difficult direct use, slow growth, etc., and achieve economic benefits and The effect of obvious social benefits, strong growth potential and fast growth speed

Inactive Publication Date: 2017-11-17
SOUTH CHINA AGRI UNIV
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Problems solved by technology

However, the polyploid induced by colchicine has a low survival rate, slow growth, difficult reproduction, and is difficult to be directly used in production.

Method used

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  • Efficient establishment method for Chinese cymbidium sexual polyploid
  • Efficient establishment method for Chinese cymbidium sexual polyploid
  • Efficient establishment method for Chinese cymbidium sexual polyploid

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Embodiment 1

[0035] Example 1: Creation of Molan Sexual Polyploid Resources

[0036] Since 2012, we have used the method described in the present invention to create Molan sexual polyploid resources. The specific method is as follows (the process is as follows: figure 1 shown):

[0037] S1. Parent selection and hybrid combination configuration: select 06-bs-45-32 (2n male gamete incidence rate 2.67) and 06-bs-45-17 (2n male gamete incidence rate 3.92) with high incidence of 2n male gametes as Parent material, on February 20, 2012, 06-bs-45-32 and 06-bs-45-17 were used as female parents, and 06-bs-45-17 was used as male parent to prepare a hybrid combination, harvested on October 18 of the same year fruit.

[0038] Among them, the methods for obtaining 06-bs-45-32 (incidence of 2n male gametes: 2.67) and 06-bs-45-17 (incidence of 2n male gametes: 3.92) with high incidence of 2n male gametes refer to the content disclosed in patent 201310668763.9.

[0039] S2. Seed culture and identifica...

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Abstract

Belonging to the technical field of plant polyploid breeding, the invention particularly discloses an efficient establishment method for a Chinese cymbidium sexual polyploidy. The method includes: selecting an efficiently occurring 2n gamete resources for hybridization, culturing hybrid seeds to form an intermediate propagule, observing the intermediate propagule, selecting quasi-like polyploidy intermediate propagule for differentiation to produce offspring seedlings, and identifying the offspring seedlings by morphological, flow cytometer and root tip chromosome tabletting so as to obtain the Chinese cymbidium sexual polyploidy. The method provided by the invention combines the plant ploidy advantage with heterosis, simultaneously establishes a technical system for efficient establishment of Chinese cymbidium sexual polyploid, and is of important significance for improving the core competitiveness and benefit of Chinese cymbidium industry in China, narrowing the gap of cymbidium breeding China and other countries as soon as possible, and promoting the efficient and sustainable development of flower industry.

Description

technical field [0001] The invention belongs to the technical field of plant polyploid breeding, and in particular relates to a method for efficiently creating sexual polyploids of orchids. Background technique [0002] Guolan (Chinese Cymbidium ) refers to some of the orchids that are produced in the temperate to tropical regions of East Asia: including Chunlan, Cymbidium, Jianlan, Molan, Hanlan, Lotus Petal, Spring Sword, Mantle Orchid, Shayelan, Qiulan Beidong Cymbidium, Emei Chunhui, Rabbit Ear Orchid, Wide-leaved Orchid, Deciduous Orchid, etc. Guolan has a pleasant aroma, beautiful plant shape and elegant flower color. It is the material carrier of Chinese traditional culture and is known as the "gentleman among flowers". It has been cultivated in my country for more than 1,000 years. With the development of Guolan industrialization and the continuous improvement of people's material and cultural living standards, Guolan began to enter the homes of ordinary people and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 曾瑞珍雷蕾郭和蓉杜国辉谢利朱娇张志胜
Owner SOUTH CHINA AGRI UNIV
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