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Method for developing marker through tetraploid potato high-throughput sequencing and application of method

A potato, polyploid technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of difficulty in molecular marker development, high frequency of genetic recombination, narrow genetic background, etc. Label densities are easily adjustable, building simple, fast, and cost-effective effects

Active Publication Date: 2016-09-07
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0002] Tetraploid potato (2n=4x=48) is highly heterozygous, has a high frequency of genetic recombination, and suffers from severe recession after selfing for many generations, but has a narrow genetic background, poor gene pool, and difficult development of molecular markers
The current genetic map mostly uses traditional markers such as SSR and AFLP, and marker development and genetic map construction are mainly carried out at the diploid level, and there are few reports on the direct development of markers at the tetraploid level

Method used

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  • Method for developing marker through tetraploid potato high-throughput sequencing and application of method
  • Method for developing marker through tetraploid potato high-throughput sequencing and application of method
  • Method for developing marker through tetraploid potato high-throughput sequencing and application of method

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Embodiment 1

[0089] Embodiment 1, a kind of method for the marker development of tetraploid potato material

[0090] 1. Investigation of the test materials and their properties

[0091] 1. Test materials

[0092] The potato tetraploid F1 generation maturity segregation population utilized in the present invention is composed of tetraploid potato early-maturing variety Zhongshu No. 3 (male parent, parent 1) and tetraploid potato late-maturing variety Zhongshu No. 19 (female parent, parent 1). Parent 2) obtained by crossing.

[0093] 2. Character survey

[0094] The maturity traits of F1 generation segregating populations were investigated. Count the times of emergence (cotyledons unearthed) and physiological maturity (plant leaves reach 50% withered and yellow), and calculate the growth period (days from emergence to physiological maturity). According to the length of the growth period, those with a growth period of less than 75 days were recorded as early-maturing materials, those with...

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Abstract

The invention discloses a method for developing a marker through tetraploid potato high-throughput sequencing and an application of the method. The method for developing the marker through the tetraploid potato high-throughput sequencing disclosed by the invention can greatly reduce the complexity of a potato genome and can facilitate operation; and by virtue of the method, high-density SNP (single nucleotide polymorphism) sites can be rapidly identified, a chromosome label difference genetic interval for controlling target character can be obtained, and finally, the molecular marker can be developed on the basis of the difference genetic interval. With the application of the method disclosed by the invention, the application of an 2b-RAD sequencing technology in potato tetraploid material marker development is successfully achieved; the difficulty on developing the potato tetraploid material marker is solved, and meanwhile, a development cycle of the character closely linked marker is greatly shortened. In addition, the method disclosed by the invention also provides a reference for genetic interval mining, marker development and genome research of other tetraploid organisms having complex genomes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for developing markers by high-throughput sequencing of tetraploid potatoes and an application thereof. Background technique [0002] Tetraploid potato (2n=4x=48) is highly heterozygous, has a high frequency of genetic recombination, and suffers from serious decline after selfing for many generations, but the genetic background is narrow, the gene pool is poor, and the development of molecular markers is difficult. Therefore, the potato genetic map has poor saturation and a small number of molecular markers, and is far behind in many aspects compared with rice, corn, soybean and other crops. The current genetic map mostly uses traditional markers such as SSR and AFLP, and marker development and genetic map construction are mainly carried out at the diploid level, and there are few reports on the development of markers directly at the tetraploid level. So far, th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李广存李兴翠金黎平徐建飞卞春松段绍光庞万福
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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