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31results about How to "Easy and fast to build" patented technology

CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-Cas9) homing sequences and primers thereof, and transgenic expression vector and establishment method thereof

ActiveCN105907758AEasy and fast to buildHigh efficiency of editing functionNucleic acid vectorFermentationEnzyme digestionNucleotide
The invention belongs to the technical field of biomedicine, and particularly relates to CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-Cas9) homing sequences and primers thereof, and a transgenic expression vector and an establishment method thereof. The CRISPR-Cas9 homing sequence sgRNA comprises SIDT1-gene-specific Target site-1 of which the target is positioned on E1 exon, and SIDT1-gene-specific Target site-2 of which the target is positioned on E2 exon, wherein the nucleotide sequence of the Target site-1 is disclosed as SEQ ID NO.8, and the nucleotide sequence of the Target site-2 is disclosed as SEQ ID NO.9. The CRISPR-Cas9 transgenic expression vector is formed by connecting the Cas9 homing sequence Target site-1 and Target site-2 to the BsmB I and Bbs I enzyme digestion sites of a recombinant shuttle Cas9 tool plasmid. The recombinant shuttle Cas9 tool plasmid can be quickly assembled with homing sequences of the two target sites of the genome, thereby packaging the recombinant adenovirus vector specifically for the two target sites; and the recombinant shuttle Cas9 tool plasmid can independently complete the editing of the large-segment genome without dependence on the synergistic actions. The establishment process is simple and quick, and has high efficiency for performing the genome large-segment editing function.
Owner:世翱(上海)生物医药科技有限公司

Back-mounted greening wall construction method

The invention discloses a back-mounted greening wall construction method, which comprises the following steps: installing a green plant pot support element in a position of a through space; installing at least one blocking element on the green plant pot support element so as to prevent green plant pot bodies from falling off towards the outside and / or the inside of the through space; installing the green plant pot bodies onto the green plant pot support element; judging whether the green plant pot bodies need maintenance operation or not; and if the maintenance operation is needed, performing the maintenance operation from the inner side of the through space. The method has the advantages that the building installation and maintenance operations can be performed on the floor of each storey of a building; the installation and the maintenance performed through setting up a high-altitude operation frame or an aerial ladder from the outside of the building are not needed; the building of a greening wall is simple, convenient and fast; and the maintenance is very simple and convenient after the greening wall is built. Adopted facilities are permanently stable, firm and reliable; the safety is high; the building cost and the maintenance cost are low; selectable plant varieties are rich; the material obtaining are easy; the greening wall can be fast formed and can be combined with the building into a whole; and the landscape is excellent.
Owner:SHANGHAI YINE GREEN TECH

Diatomite reactor device

The invention relates to a diatomite reactor device. An outer shell (1) of the device is composed of an upper octagonal column body and a lower octagonal cone body, eight supporting columns (2) are arranged on the eight corners of the outer side of the octagonal column body for supporting, gutters (9) are horizontally formed in the inner side of the upper octagonal column body of the outer shell (1) along the circumference, at least four stand columns (4) are arranged on slopes of the lower octagonal cone body of the outer shell (1), hollow cylinders (5) are fixedly connected to the top ends of the stand columns (4), the internal area defined by the stand columns (4) and the hollow cylinders (5) is a flocculation chamber (6), the external area defined by the stand columns (4) and the hollow cylinders (5) is a clarifying chamber (7), an ultrafiltration layer (20) is arranged in the clarifying chamber (7), a unit weir groove net (8) is arranged on the top of the clarifying chamber (7), a gradually-expanding guide cylinder (10) is arranged in the flocculation chamber (6), the bottom of the gradually-expanding guide cylinder (10) is connected with a water inlet pipe (11) which is connected with a pipeline mounted pump (12), sludge buckets (13) are arranged on the middle-lower portions of the slopes of the clarifying chamber (7), sludge discharging pipes (14) are connected in the sludge buckets (13), and the sludge discharging pipes (14) are connected to a sludge storage pool, and a return pipe (15) is arranged at the inner bottom of the device and connected to a biochemical pool anoxic zone. The diatomite reactor device is simple in structure, good in sewage processing effect, small in occupied space, low in investment and attractive and elegant in appearance.
Owner:SHANXI KEYUAN SHENGDI TECH

Method for quickly establishing ad hoc network by using unmanned aerial vehicle projection

The invention provides a method for quickly establishing an ad hoc network by utilizing unmanned aerial vehicle projection. A ground console and an unmanned aerial vehicle provided with a base stationprojection device are included; the unmanned aerial vehicle receives a network distribution instruction and network distribution plan area information sent by the ground console, and executes networkdistribution work; the unmanned aerial vehicle plans a first projection point according to a network layout planning area and flies to a first projection point for first projection; after the first projection is completed, the unmanned aerial vehicle plans a second projection point according to the signal coverage range of the base stations, and flies to the second projection point for second projection; the projection actions are repeated until the ad hoc network signal range formed by the projection base stations covers the network distribution planning area; after ad-hoc network distribution is completed, the unmanned aerial vehicle is switched from GPS positioning to ad-hoc network signal positioning. According to the method, the advantages of the unmanned aerial vehicle can be exerted, the wirelessly-controlled ad hoc network base stations are arranged outdoors in a projection mode, ad hoc network construction in a severe environment is achieved, and the base station constructionprocess is rapid and convenient.
Owner:深圳市贝贝特科技实业有限公司

Prefabricated concrete structure beam column connecting joint, connecting method and repairing method

The invention discloses a prefabricated concrete structure beam column connecting joint, a connecting method and a repairing method. The prefabricated concrete structure beam column connecting joint comprises a joint plate, prefabricated beams connected to the left side and the right side of the joint plate, an upper-layer prefabricated column connected to the upper end of the joint plate and a lower-layer prefabricated column connected to the lower end of the joint plate; connecting blocks and groove-shaped joints are arranged between the joint plate and the prefabricated beams; and the joint plate, the connecting blocks and the groove-shaped joints are connected into a whole through main steel stranded wires. The prefabricated concrete structure beam column connecting joint has the characteristic of self-resetting, residual deformation after an earthquake is small, and it is guaranteed that the building use function of the structure can be rapidly recovered after the earthquake. Meanwhile, the damage to the structure is limited in the connecting blocks which can be easily replaced or repaired, so that repairing after the earthquake is quick and convenient. In addition, the whole structure is composed of detachable parts, and the sustainable development performance of a building can be improved by conveniently disassembling and recycling prefabricated parts.
Owner:SOUTHEAST UNIV

Power supply path construction method and system based on transformer substation and storage medium

The invention discloses a power supply path construction method and system based on a transformer substation and a storage medium. The power supply path construction method based on the transformer substation comprises the steps: acquiring equipment information from starting point equipment to multiple pieces of pre-connection equipment of the transformer substation; obtaining an equipment parameter and an equipment connection relationship according to the equipment information; filtering the plurality of pieces of pre-connected equipment according to the equipment parameters and the equipmentconnection relationship to obtain equipment to be connected; and connecting any two piece of to-be-connected equipment according to the device connection relationship to construct a power supply path. According to the method and system, after the multiple pieces of pre-connection equipment from the starting equipment to the transformer substation are obtained, the multiple pieces of pre-connection equipment are filtered according to the equipment parameters and the equipment connection relationship to obtain the to-be-connected equipment, and then any two pieces of to-be-connected equipment are connected according to the equipment connection relationship to construct the power supply path, so that the power supply path is simple and rapid to construct; and manpower is saved.
Owner:SHENZHEN POWER SUPPLY BUREAU

Construction method of back-mounted green wall

The invention discloses a back-mounted greening wall construction method, which comprises the following steps: installing a green plant pot support element in a position of a through space; installing at least one blocking element on the green plant pot support element so as to prevent green plant pot bodies from falling off towards the outside and / or the inside of the through space; installing the green plant pot bodies onto the green plant pot support element; judging whether the green plant pot bodies need maintenance operation or not; and if the maintenance operation is needed, performing the maintenance operation from the inner side of the through space. The method has the advantages that the building installation and maintenance operations can be performed on the floor of each storey of a building; the installation and the maintenance performed through setting up a high-altitude operation frame or an aerial ladder from the outside of the building are not needed; the building of a greening wall is simple, convenient and fast; and the maintenance is very simple and convenient after the greening wall is built. Adopted facilities are permanently stable, firm and reliable; the safety is high; the building cost and the maintenance cost are low; selectable plant varieties are rich; the material obtaining are easy; the greening wall can be fast formed and can be combined with the building into a whole; and the landscape is excellent.
Owner:SHANGHAI YINE GREEN TECH

Liquid vapor floating electric generator

A liquid vapor floating electric generator is mounted below the horizontal plane and provided with a pair of rotating wheels connected from top to bottom, wherein an output power shaft is arranged at the center of each of the rotating wheels; the output power shafts can drive the electric generator to work to generate electricity; the pair of rotating wheels are in transmission connection through a caterpillar track; a caterpillar track air tube group is arranged on the periphery of the caterpillar track in sequence; inflatable buoys and airless buoys are mounted on the caterpillar track air tube group correspondingly in a matched manner; air tubes are mounted on each of the inflatable buoys and the airless buoys and communicated correspondingly; a photoelectric air valve is mounted at the connection part of each of the buoys and the caterpillar track air tube group; an air valve switch and a hook are mounted on each of the photoelectric air valves; a ground hook is fixedly mounted below the liquid vapor floating electric generator and used for poking the air valve switches. The liquid vapor floating electric generator is simple and compact in structure, convenient and fast to build, applicable to all kinds of hydroelectric power stations, not only low in investment cost, but also short in production cycle and capable of protecting the environment, and moreover has the advantages of high power generation efficiency, low power generation cost, high unit start-up speed and easiness of adjustment.
Owner:刘德林

A kind of excrement cleaning device for aquaculture pond

The invention discloses a culture pond fecal cleaning device for aquaculture. As a finished part, the culture pond fecal cleaning device for the aquaculture can be directly used for constructing a culture pond; quick construction of the culture pond is facilitated; the problem of arrangement of a drainage pipeline of a pond body is not required to be considered any longer; the time consumption caused by the construction of the culture pond is reduced; after the culture pond fecal cleaning device for the aquaculture is arranged in the culture pond, the deposition of fecal or residual bait is not formed easily; the communication of a fecal removal drawer and the pond body is blocked in a sealed manner by utilizing a movable water partition plate when the fecal is required to be cleaned, andthe fecal removal drawer can be withdrawn from the front side of the pond body, so that the fecal is cleaned conveniently and quickly; moreover, after the culture pond fecal cleaning device for the aquaculture is arranged in the culture pond, if the culture pond is required to be discharged, the fecal can be removed first; on the basis of withdrawing the fecal removal drawer, the movable water partition plate is controlled to move backwards electrically, so that a drainage gap is formed in the bottom of the pond body, and water is drained quickly; a working box can also be washed by drained water by the way, and the pollution caused by that fact that the fecal overflows into the working box when the fecal removal drawer is withdrawn is eliminated.
Owner:FRESHWATER FISHERIES RES INST OF SHANDONG PROVINCE

A method for developing markers by high-throughput sequencing of tetraploid potato and its application

The invention discloses a method for developing a marker through tetraploid potato high-throughput sequencing and an application of the method. The method for developing the marker through the tetraploid potato high-throughput sequencing disclosed by the invention can greatly reduce the complexity of a potato genome and can facilitate operation; and by virtue of the method, high-density SNP (single nucleotide polymorphism) sites can be rapidly identified, a chromosome label difference genetic interval for controlling target character can be obtained, and finally, the molecular marker can be developed on the basis of the difference genetic interval. With the application of the method disclosed by the invention, the application of an 2b-RAD sequencing technology in potato tetraploid material marker development is successfully achieved; the difficulty on developing the potato tetraploid material marker is solved, and meanwhile, a development cycle of the character closely linked marker is greatly shortened. In addition, the method disclosed by the invention also provides a reference for genetic interval mining, marker development and genome research of other tetraploid organisms having complex genomes.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI

A prefabricated concrete structure beam-column connection node, connection method and repair method

The invention discloses a prefabricated concrete structure beam-column connection node, a connection method and a repair method, wherein the prefabricated concrete structure beam-column connection node includes a joint plate, prefabricated beams connected to the left and right sides of the joint plate, and an upper layer connected to the top of the joint plate The prefabricated column and the lower prefabricated column connected to the lower end of the gusset plate; connecting blocks and grooved joints are arranged between the gusset plate and the prefabricated beam; the gusset plate, the connecting block and the grooved joint are connected as a whole through main steel strands. The beam-column connection node of the prefabricated concrete structure of the present invention has the characteristics of self-resetting, and the residual deformation after the earthquake is small, so as to ensure that the structure can quickly recover its building function after the earthquake. At the same time, the damage of the structure is limited to the connecting blocks that can be easily replaced or repaired, making it quick and easy to repair after the earthquake. In addition, the structure is all composed of detachable parts, and the sustainable development performance of the building can be improved through the convenient disassembly and recycling of prefabricated components.
Owner:SOUTHEAST UNIV

Manufacturing method for composite foamed cement ultra-large plate

A manufacturing method for a composite foamed cement ultra-large plate includes the following steps that a, prefabricated foamed cement plates are used for building a composite foamed cement ultra-large plate body of a needed size, transverse staggered type splicing is adopted for a building manner, and the prefabricated foamed cement plates are bonded through high-strength mortar; b, the four sides of the built composite foamed cement ultra-large plate body are sleeved with right-angled-U-shaped groove steel for edge covering; c, gridding cloth is pasted to the front plate face and the rear plate face of the built composite foamed cement ultra-large plate through high-strength mortar; d, three or more steel wires are used for bundling the upper and lower right-angled-U-shaped groove steel and the left and right right-angled-U-shaped groove steel; and e, gridding cloth is pasted to the front plate face and the rear plate face of the composite foamed cement ultra-large plate through high-strength mortar. By applying the method, field manufacturing of the composite foamed cement ultra-large plate which is 3-16 meters long and 2-8 meters wide can be achieved in a construction site, and the composite foamed cement ultra-large plate can be widely applied to villa roofs, single-storey house roofs and the topmost layer roofs of buildings of two or more storeys and construction of assembly type house walls.
Owner:GUANGZHOU QINGNENG BUILDING MATERIALS CO LTD
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