Plasmid standard molecule capable of being used for detecting multiple crops and multiple exogenous genes

Abstract

The invention discloses a plasmid standard molecule pLANGUTOU-5 capable of being used for detecting multiple crops and multiple exogenous genes and application thereof. The base sequence of the plasmid standard molecule is shown as SEQ ID NO: 1. The pLANGUTOU-5 is used as a positive control or a positive standard substance, 13 species and 39 exogenous genes can be detected, the problem that a transgenic positive material is difficult to obtain is solved, the exogenous gene detection range is greatly enlarged, the risk of missing detection is reduced, and the requirement for preparing multiple positive controls is also reduced. The plasmid constructed through the method can be applied to 13 main crops and economic crops, the variety of detected crops is greatly enlarged, and transgenic monitoring and safety supervision are facilitated. The plasmid molecule provided through the method is easy and fast to construct and low in cost, and the transgenic detection cost can be greatly reduced.

Description

Technical field[0001]The present invention belongs to the field of transgenic detection, and is specifically involved in a plasmid standard molecule which can be used to detect a variety of crops and a plurality of foreign genes.Background technique[0002]GM product safety has been attracted, and the molecular detection of transgenic crops and its products is increasingly important. At present, countries have different requirements for genetically modified products, and some national provisions must indicate the content of transgenic components, and the requirements of each country have different requirements for the identified transgenic content. In order to solve the problem of trade disputes, the qualitative and quantitative detection of transgenic products become critical (Dong Lianhua, etc.). The detection method of currently used transgenic crops is based on sample DNA detection methods and method based on sample proteins. Sample protein-based detection methods include Enzyme-L...

AI Technical Summary

Problems solved by technology

There are hundreds of genetically modified strains that have passed safety evaluation or have been commercialized in various countries around the world, but many genetically modified strains have not been commercially produced in China

It is difficult to obtain plant materials of these strains. The current commercial GMO detection standards are mainly matrix reference materials. The development process of matrix reference materials is relatively complicated and the price is very expensive. Each gram of matrix reference material powder is about 100-200 euros

Therefore, the purchase of reference materials is a heavy burden for institutions that want to conduct genetically modified testing

In addition, the development of domestic standard products is relatively lagging behind. If you want to buy other standard products, you need to import them. Because of the genetic modification, the procedures are cumbersome and the cycle is long.

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