25 results about "Biological Testing" patented technology

The invention discloses a quintuple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit for simultaneously detecting five parasites as well as special primers and probes thereof, and belongs to the technical field of biological detection. The kit provided by the invention can be used for simultaneously detecting cryptosporidium parvum, strongyloid stercoides, trypanosoma kirschneri, theileria and echinococcus granulosus in the same system, and has the characteristics of qualitative and quantitative properties, high sensitivity, strong specificity, good repeatability and good stability.

The invention discloses an acetic acid content determination kit with an oscillation structure, and relates to the technical field of biological detection, the acetic acid content determination kit comprises a kit body and a protective top cover hinged to the rear of the kit body; a cleaning mechanism is arranged on the inner side of the protective top cover, the cleaning mechanism comprises a cleaning brush rod, and the bottom end of the cleaning brush rod slidably penetrates through the left side and the right side of the bottom end of the interior of the protective top cover; wherein an oscillation mechanism is arranged in an oscillation cavity in the kit body, the oscillation mechanism comprises a guide positioning disc, and the guide positioning disc is fixedly mounted at the lower part in the oscillation cavity. According to the acetic acid content determination kit with the oscillation structure, the cleaning mechanism cleans the determination contact pad through overturning of the protective top cover, reagent overflow is prevented while residues are avoided, the reagent tube is driven to rotate by adopting the intermittent fast and slow rotating oscillation mechanism, oscillation is realized by adopting an abutting mode, and the detection accuracy is improved. And the uniformity is prevented from being influenced by a single oscillation mixing mode.

Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances.

The invention discloses a method for determining a micro-plastic particle seawater ecological risk evaluation threshold value. The method comprises the following steps: (1) preparing pure micro-plastic; (2) carrying out biological testing; and (3) deducing the threshold value. According to the method, the toxic effect of pure micro-plastic can be measured, instead of the composite toxic effect of the micro-plastic and the leaching additive, and the toxicity of the micro-plastic particles to marine organisms can be evaluated more accurately; in addition, the tested organisms adopted by the method are marine organisms, so that the risk of the micro-plastic particles to seawater ecology can be reflected more accurately; the method can also derive the toxicity threshold value of the micro-plastic particles to marine organisms.

The invention belongs to the field of biological detection, and discloses an efficient and rapid concentration method and device for LPS or LAM. The inventor finds out that the non-polar macroporous resin with a specific aperture range has similar affinity adsorption efficiency to lipopolysaccharide or lipoarabinomannan, the adsorption capacity is large, the adsorption speed is high, the lipopolysaccharide can be efficiently eluted in cooperation with a specific eluent, a concentrated lipopolysaccharide to-be-detected sample is obtained, and the non-polar macroporous resin with the specific aperture range can be used for detecting the lipopolysaccharide or lipoarabinomannan in the lipopolysaccharide or lipoarabinomannan in the lipopolysaccharide or lipoarabinomannan. And a good condition is created for the detection of lipopolysaccharide or lipoarabinomannan. Efficient concentration of LAM is realized by using the method, and excellent conditions are provided for detection of mycobacterium tuberculosis.

The invention belongs to the technical field of biological detection, and particularly relates to an SNP (Single Nucleotide Polymorphism) site for distinguishing a mycoplasma synoviae MS-H vaccine strain and a wild strain, a kit and application, and target genes meeting detection requirements are screened out by carrying out bioinformatics comparative analysis on whole genomes of MS-H and parent strains thereof. A specific primer and an MGB probe are designed for a specific conserved gene sequence ktrB gene for the first time, and the double qPCR kit for distinguishing the MS-H vaccine strain and the wild strain is established. In-vitro experiments prove that the kit disclosed by the invention is simple to operate, high in sensitivity, strong in specificity and good in repeatability. The screened gene loci and the established detection kit have the advantages that the defect that at present, common genes are prone to reverse mutation is overcome, the method can be used for clinical rapid diagnosis of MS vaccine strains and wild strains, the detection accuracy is improved, population purification is promoted, and certain commercial value is achieved.

The utility model relates to the technical field of microbiological examination, and provides an anaerobic organism culture bin for biological examination, the anaerobic organism culture bin comprises a box body, a partition plate and a driving assembly, the box body is provided with a culture cavity and a taking-out cavity, the volume of the culture cavity is smaller than that of the taking-out cavity, and the partition plate is used for separating the culture cavity from the taking-out cavity; an operation table is mounted in the culture cavity, and the driving assembly is used for driving the operation table to enter the taking-out cavity. According to the anaerobic organism culture bin for biological examination, the amount of nitrogen filled into the culture box again after anaerobic organisms in the culture box are sampled is reduced.

A kit for diagnosing a biological sample from a user is disclosed. The kit includes a mobile application that may run on a mobile device, a collection container for the biological sample, a cartridge that docks with the collection container and a reader that docks with the cartridge and that optically reads the biological sample.

The utility model relates to a biological assay device for arthropods, and discloses a device for carrying out biological assay on spider mites on living plant leaves, which comprises a base (1), a telescopic device (2) and a storage platform (3). A fixing hole (11) is formed in the base (1); the telescopic device (2) is connected with the base (1) through a bolt, the telescopic device (2) comprises an outer pipe (22) and an inner pipe (21), and the outer pipe (22) is provided with a fixing bolt used for fixing the relative position of the inner pipe (21); the storage platform (3) is fixedly connected with the upper part of the inner pipe (21); the utility model has the advantages that the leaf is not easy to damage, the system stability is good, the position of the culture dish is adjustable, the whole device is easy to move, the observation is easy, and the like, and can better complete the biological assay test of the tetranychid on the living plant leaf.

The present application belongs to the field of biological testing. Disclosed are a multi-joint test quantum dot quantitative test cassette, a preparation method and device therefor, and a use method therefor. The multi-joint test quantum dot quantitative test cassette comprises a multi-joint-row cassette shell and test strips, wherein a plurality of reaction cavities are provided in the multi-joint-row cassette shell and are used for the placement of the test strips; the multi-joint-row cassette shell further comprises a plurality of sample-adding holes and a plurality of observation windows, the sample-adding holes and the observation windows corresponding to the reaction cavities on a one-to-one basis; and each test strip comprises a binding pad, which is coated with an antibody marked by quantum dots, the antibody marked by the quantum dots serving as a chromogenic substance. The present application provides a multi-joint test quantum dot quantitative test cassette, which uses quantum-dot-marked quantitative testing, a nitrocellulose membrane with a plurality of test lines, and the form of the multi-joint-row cassette shell. Thus, the problems of quantitative testing and multi-joint testing are both solved, and the test efficiency and accuracy are improved; furthermore, the present application achieves the aim of rapidly diagnosing various allergic diseases of a human body that are caused by the inhalation and ingestion of allergens.

A non-healthcare setting kit for diagnosing a biological sample from a user is disclosed. The kit includes a mobile application that may run on a mobile device, a collection container for the biological sample, a cartridge that docks with the collection container and a reader that docks with the cartridge and that optically reads the biological sample.

The present invention belongs to the field of biological testing, specifically relating to a nucleic acid extraction device and a method for extracting and amplifying target nucleic acids using the device. The device comprises a nucleic acid extraction element, a waste liquid storage chamber, and a reaction chamber. The reaction chamber selectively communicates with the nucleic acid extraction element and the waste liquid storage chamber, and fluid exchange occurs through pressure fluctuations between the waste liquid storage chamber and the reaction chamber. The present invention utilizes an integrated control system that discharges fluid from the reaction chamber into the waste liquid storage chamber under negative pressure, resulting in convenient operation and high efficiency.

The invention relates to the technical field of biological detection, in particular to development of a DTT-mediated CRISPR / Cas12a system and HPV (human papillomavirus) detection application. The DTT-mediated CRISPR / Cas12a system is constructed, the PAM-sequence-free nucleic acid is targeted, the nucleic acid detection range of the CRISPR / Cas12a system is remarkably expanded, and meanwhile the analysis sensitivity and specificity of the CRISPR / Cas12a system are improved.

The invention relates to a sample extraction device for biological testing in the field of sample extraction devices. The sample extraction device comprises a test tube rack, a lifting rack is arranged on the outside, a control box is arranged on the fixed end, a horizontal guide rail is arranged on the top end, a longitudinal guide rail is arranged on the movable end, three kinds of mounting holes are arranged on the test tube rack, auxiliary extraction structures are arranged in the mounting holes, washing tubes and elution tubes are arranged in the other two mounting holes, the auxiliary extraction structure comprises a lifting platform, a supporting column and an annular magnetic column, the annular magnetic column has a plurality of pairs of arc-shaped electromagnets, the movable end of the longitudinal guide rail has an auxiliary extraction mechanism, comprising a shell, a motor and a stirring rod, the stirring rod has a main shaft, a segment block and an electromagnet ring, a control electric board is arranged in the shell, and image acquisition devices are arranged on the side end and the upper side of the test tube rack. By combining image recognition, automatic control and the design of the auxiliary extraction structure, the biomagnetic beads can efficiently adsorb biomass during the extraction process, and the biomagnetic beads are not easily missed during the transfer process, so that the biomagnetic beads can extract biomass efficiently, accurately and automatically.

The invention belongs to the technical field of chromatographic separation and preparation, and particularly relates to a centrifugal column chromatographic extraction device and method. The device comprises a chromatographic column, an extraction bin, a top cover box, a tubular column and an external connecting assembly, the top cover box and the external connecting assembly are arranged at the top and the bottom of the extraction bin, the tubular column penetrates through the top cover box and is rotationally connected with the top cover box through a bearing assembly, the chromatographic column is contained in the extraction bin and connected with the tubular column, and a sample solution is injected into the chromatographic column through the tubular column. Then pressurizing and injecting a mobile phase, driving the chromatographic column to rotate by the tubular column for chromatographic separation, pressurizing and injecting an extracting solution into the extraction bin, and discharging the separated mobile phase by the extracting solution through an external connecting assembly. While pressurized separation is carried out, high-speed rotating centrifugation of the chromatographic column is utilized, centrifugal force is used as auxiliary drive, mass transfer resistance is reduced, separation efficiency is improved, and a high-efficiency mode and way can be provided for rapid separation and extraction of samples in life science, biological detection and food and drug detection.

The present invention provides a chip-based reagent box for biological testing and a detection method thereof. The detection device includes a flow path guide layer and is stacked with a substrate to form a main body, and a reaction area is provided on a part of a pair of electrodes on the substrate, and a receptor that binds to a detection target is applied to the reaction area. The flow path guide layer has an opening installed at the same position as the reaction area, and the receptor is exposed to the main body at the opening, and a micro flow path is formed between the flow path guide layer and the substrate. The detection method includes steps of dropping, washing, and detection, and the sample liquid is directly dropped into the reaction area from the opening to react with the receptor. After the reaction time, a washing liquid is added to the sample liquid and dropped into the reaction area, which is permeated into the micro flow path by capillary action and discharged, and then the test liquid is dropped into the reaction area to detect the sample. In this method, the sample detection can be completed without applying external force, and the detection efficiency can be improved.

The invention relates to the technical field of biological detection, in particular to a sugar deficiency transferrin detection method. The invention relates to a method for detecting sugar-deficient transferrin. The method comprises the following steps: specifically separating sugar-deficient transferrin in a detection sample by using Fe3O4 magnetic nanoparticles coupled with an anti-sugar-deficient transferrin antibody; washing and eluting; labeling with a fluorescent dye; fluorescence detection; and calculating the concentration of the sugar deficiency transferrin. The Fe3O4 magnetic nanoparticles coupled with the anti-sugar-deletion transferrin antibody are used for performing specific separation on the sugar-deletion transferrin antibody in a detection sample, the antibody on the magnetic nanoparticles can efficiently capture the sugar-deletion transferrin, the detection sensitivity is improved, the sugar-deletion transferrin with lower concentration can be detected, and the detection sensitivity is improved. By utilizing the specific binding capacity of the anti-sugar-deficiency transferrin antibody, the influence of other interfering substances in a sample is effectively eliminated, and the detection specificity is improved.

The present invention relates to the field of biological testing. Disclosed are a method for efficient and rapid concentration of lipopolysaccharide (LPS) or lipoarabinomannan (LAM), and an apparatus. The inventor has found by researches that a non-polar macroporous resin within a specific pore size range exhibits adsorption efficiency for LPS or LAM comparable to affinity adsorption, has a large adsorption capacity and a high adsorption rate, and can efficiently elute LPS in combination with a specific eluent to obtain a concentrated LPS sample under test, thereby creating good conditions for the detection of LPS or LAM. By means of the method, efficient concentration of LAM is achieved, providing excellent conditions for the detection of mycobacterium tuberculosis.

A method and system for performing assays for hemoglobin HbA1c, employing a smart phone that interacts with a disposable test strip coated with dry chemistry in physically and chemically segregated reactive zones. In the given regions of the strip, the chemistry interacts with the sample resulting in the conversion of the analyte, such as glycated hemoglobin, into a complexed substrate which can be quantified optically. The phone takes measurements of various parameters during the process of the test reactions, displays relevant instructions and the assay results.

The utility model relates to the technical field of biological inspection instruments, and discloses an independent defrosting biological inspection material cabinet which comprises a box body, a box cover, a cooling mechanism and a ventilation mechanism. The cooling mechanism comprises a hollow base, an inner container, a condenser, a fan and a filter screen. The ventilation mechanism is composed of a sliding frame, a motor, a gear, a baffle and the like. The material inspection cabinet is linked with the single chip microcomputer through the temperature sensor, when the temperature of the inner container is low and the inner wall is frosted, the fan accelerates to operate, external air enters the space between the box body and the inner container through the hollowed-out base, meanwhile, the motor drives the baffle to seal the notch in the bottom of the inner container, the refrigeration effect of the condenser is reduced, and heat-containing air enters the inner container through the filter screen to be defrosted. During cooling, the baffle moves out of the notch, the distance of cold air entering the inner container is shortened, the fan accelerates to enable the cold air to rapidly circulate, efficient cooling is achieved, and storage safety of biological samples is guaranteed.

The invention provides a microbiological detection method and a microbiological detection device, and relates to the technical field of biological detection. The microbiological detection method comprises the following steps: modifying a dendritic nucleic acid skeleton to the surface of a magnetic bead through a biotin-streptavidin system to form an aptamer-magnetic bead-peptide nucleic acid skeleton complex; the aptamer-magnetic bead-peptide nucleic acid skeleton complex is applied to microbiological detection based on a micro-fluidic chip. The microbiological detection method disclosed by the invention can achieve the technical effects of rapidness, high sensitivity, high selectivity, low cost and full-automatic microbiological detection, and the corresponding microbiological detection device has the characteristics of portability, wide generalization and low professional requirements on operators.

The invention discloses a plastic foam toxicity evaluation method combining light aging simulation and biological detection, and belongs to the technical field of environmental health and microplastic toxicology. The method comprises the following steps: firstly, carrying out accelerated light aging on plastic foam particles under a simulated sunlight condition, and characterizing physicochemical properties (including surface morphology, particle size distribution and functional group change) of the plastic foam particles; then, carrying out toxicity detection by utilizing a model organism caenorhabditis elegans, and systematically evaluating the biological effect of the light aging EPS through indexes such as egg laying amount, movement behavior and body length. According to the method, the light aging process of the plastic foam in the environment can be truly represented, the incidence relation between the physicochemical change and the toxicity effect can be established, and an effective experimental basis and methodological support are provided for scientific evaluation of the environmental health risk of the plastic foam and product safety supervision.

The application discloses a method for determining a seawater ecological risk evaluation threshold of microplastic particles, and comprises the following steps: (1) preparing pure microplastics, (2) biological testing, and (3) threshold derivation. Through the method, the toxic effect of pure microplastics can be determined instead of the combined toxic effect of microplastics and leaching additives, so that the toxicity of microplastic particles to marine organisms can be more accurately evaluated. In addition, the tested organisms used in the method are all marine organisms, so that the risk of microplastic particles to seawater ecology can be more accurately reflected. The method can also derive the toxicity threshold of microplastic particles to marine organisms.

To provide solid state ion selective electrodes.SOLUTION: An ion selective electrode (ISE) assembly may be installed within a sample path of a biological testing system to measure ion concentration within a sample fluid flowing through the sample path. A transducer membrane is placed within a housing and positioned to contact the sample path. A threaded portion is advanced into a socket of the housing, and forces the membrane against a sealing portion of the sample path to prevent retention of the sample fluid within the housing after testing. Sealing may be accomplished without adhesives or sealants, and instead relies upon mechanical pressure of the threaded portion. Another implementation includes a reference measuring electrode and several ion measuring electrodes combined into a single housing. Another implementation includes the reference measuring electrode and several ion measuring electrodes combined into a cartridge that includes a sample well instead of the sample path.SELECTED DRAWING: None

The present invention relates to a disinfection supply detection method and system, which relates to the field of disinfection detection. The method comprises the following steps: obtaining the categories and quantities of medical devices being disinfected in a disinfection supply room to obtain a distribution of device categories; classifying the device complexity based on the device category distribution to obtain the device complexity; and calculating the number of random inspections of the device disinfection test based on the device complexity to obtain the number of random inspections and randomly sampling the medical devices in the number of random inspections to obtain a set of medical device images; and identifying surface damage on the medical device image set to obtain a set of surface damage parameters. In combination with the device complexity, the method calculates the number of random inspections of the damaged area to obtain the number of random inspections of the damaged area and performs random biological testing on the sampled medical devices to obtain test results. The present invention solves the technical problems of inaccurate, low reliability, and low efficiency in post-disinfection supply detection.