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Culture medium its rpeparation method and method for culturing lact-streptocin

A nisin and culture medium technology is applied in the field of nisin cultivation and nisin production medium, and can solve problems such as affecting the normal fermentation of nisin, single nutrient composition, and difficulty in quality control.

Active Publication Date: 2005-10-26
ZHEJIANG SILVER ELEPHANT BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned culture medium can ensure the basic metabolism of Lactococcus lactis and the synthesis of nisin, milk and beef extract are animal proteins, the cost is high, the raw materials are affected by the place of origin, and the quality control is difficult. It is often necessary to add some antiseptic preservatives, which will affect the normal fermentation of Streptococcus lactis
At the same time, the finished product will be hindered in the Kosher (Jewish) and other related certifications, and it will be difficult to pass, which will directly affect the application range of the product
Although soybean peptone has a relatively small molecule, its nutrient composition tends to be single, and the manufacturing process also causes the loss of certain nutrients such as trace elements and carbon sources
Therefore, the medium has yet to be optimized and improved to meet the requirements of increasing product yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Include the following raw materials in weight percentage:

[0030] Yeast powder: 0.5%, soybean powder: 0.9%, glucose 1.0%, the rest is deionized water.

[0031] Put yeast powder and soybean powder into the batching tank, add deionized water and stir well, adjust the pH to 6.2 with 29% edible hydrochloric acid, then send it into the culture tank, add deionized water, and feed saturated high-pressure steam to maintain the pressure in the tank at 0.10 MPa, sterilize at a temperature of 118°C for 35 minutes, then indirectly cool to 25°C with cooling water through a cooling coil.

[0032] The inoculum amount of food-grade lactic acid bacteria inserted into the above medium is 0.3‰. Use sterile air to maintain the pressure in the culture tank at 0.5MPa and culture at 26°C for 72 hours, and the nisin activity can be stopped when it reaches 4000IU / ml as determined by the biological titer.

[0033] The above-mentioned yeast powder is provided by Shanghai Xianchen Industrial Co...

Embodiment 2

[0037] Include the following raw materials in weight percentage:

[0038] Yeast powder: 0.5%, soybean protein isolate: 0.5%, sucrose: 3.0%, sodium phosphate: 0.5%, and the rest is well water.

[0039] Put yeast powder and soybean protein isolate into the batching tank, add well water and stir evenly, add sodium phosphate, adjust the pH to 7.0 with 31% edible lye, and then send it into the culture tank, add well water, and feed saturated high-pressure steam to maintain The pressure in the tank is 0.10 MPa, and the temperature is 121 ° C for 30 minutes, and the cooling water is indirectly cooled to 30 ° C through the cooling coil.

[0040] The inoculum amount of food-grade lactic acid bacteria inserted into the above medium is 0.5‰. Use sterile air to maintain the pressure in the culture tank at 0.1MPa and culture at 37°C for 24 hours. When the nisin activity reaches 10,000 IU / ml as determined by the biological titer, it can be stopped.

[0041] The above-mentioned yeast powde...

Embodiment 3

[0045] Include the following raw materials in weight percentage:

[0046] Yeast extract: 1%, soybean hydrolyzate: 0.3%, maltose: 1.5%, sodium phosphate: 0.1%, magnesium sulfate 0.5%, and the rest is tap water.

[0047]Put the yeast extract and soybean hydrolyzate into the batching tank, add tap water and stir evenly, add sodium phosphate and magnesium sulfate, adjust the pH to 7.2 with 30% edible lye, send it into the culture tank, add tap water, and feed it into saturated high pressure Steam to maintain the pressure in the tank at 0.09MPa, sterilize at 120°C for 35 minutes, and then indirectly cool to 35°C with cooling water through the cooling coil.

[0048] The inoculation amount of food-grade lactic acid bacteria inserted into the above medium is 1.0‰. Use sterile air to maintain the pressure in the culture tank at 0.3 MPa and culture at 30°C for 48 hours, and the nisin activity can be stopped when it reaches 15,000 IU / ml as determined by the biological titer.

[0049] T...

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PUM

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Abstract

The present invention discloses a culture medium for producing lact-streptocin nisin its methods of preparation and method for culturing nisin. The preparation is put yeast protein, soy bean protein into jar, add water and mix, then put carbon, phosphate in turn, mix, debug PH6.2 - 7.2 into jar, add water, and high pressure steam, bactericide, cooling, connect food lactacide, breeding under the condiction of pressure is 0.1 - 0.5 MPa, temperature is 26 - 37 Deg. C for 24 - 72 hours, by biological testing the active of nisin is up to 4000 - 15000 IU / ml, then stop.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for producing nisin, a preparation method thereof and a method for cultivating nisin. technical background [0002] Nisin is a small peptide produced by certain Lactococcus lactis with a molecular weight of about 3510Da. This bacterium has a strong inhibitory effect on many Gram-positive bacteria, especially Bacillus, Clostridium, Staphylococcus, Listeria, etc. that cause food spoilage, and because it can be degraded by enzymes in the digestive tract, it is a It is a natural food preservative that is non-toxic to the human body, so it is widely used as a preservative and preservative for dairy products, canned foods, meat products and beverages. [0003] In the research of the Institute of Microbiology, Chinese Academy of Sciences, Lactococcus lactis AL2, a high-yielding mutant strain of lactococcus lactis, was used as the production strain, and cheap raw materials such as pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/02
CPCC12N1/20C12P21/02
Inventor 沈颜新范悠然
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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