10 results about "Streptocin" patented technology

The invention belongs to the technical field of medical gel, and particularly relates to gynecological antibacterial gel and a preparation method thereof. The gel comprises an antibacterial phase and a bacterium promoting phase, and broad-spectrum inhibition of common pathogenic bacteria is achieved through the synergistic proportion of naringin, nisin and ethyl lauroyl arginate hydrochloride; meanwhile, a lactobacillus rhamnosus metabolite, sodium hyaluronate and the like are introduced to construct a bacterium promoting phase, and probiotic colonization and vagina micro-ecological balance reconstruction are promoted. The pH value of the gel is stabilized at 4.0-4.5, and the gel has good rheological properties and retention performance. In the preparation method, all the components are added step by step, the system temperature and stirring conditions are controlled, and the component activity and the product stability are guaranteed. The traditional Chinese medicine composition can effectively relieve bacterial vaginitis, fungal infection and micro-ecological disorder, and has a synergistic, safe and mild comprehensive nursing effect.

The invention relates to recombinant escherichia coli and application thereof in production of nisin, and belongs to the technical field of biology. According to the invention, a recombinant escherichia coli system capable of efficiently producing Nisin is successfully constructed by heterologous expression of precursor peptide, dehydratase NisB and cyclase NisC of a Nisin synthesis route in escherichia coli. Meanwhile, a dissolution promoting label is adopted, so that the activity and the stability of Nisin key modification enzyme NisB are remarkably improved. The expression quantity of Nisin is improved by screening a promoter and an expression vector. Finally, through systematic optimization of fermentation conditions, the yield of Nisin is remarkably increased, the titer can reach 25342 IU / mL, and a new technical route is provided for industrial efficient production of Nisin.

The invention relates to the technical field of roast egg preservation, in particular to high-temperature-resistant modified embedded nisin and application thereof in high-temperature roast egg processing. Comprising nisin, hydroxypropyl-beta-cyclodextrin wrapping the outer side of a non-bacteriostatic hydrophobic region of the nisin, and non-denatured type I collagen wrapping the outer sides of the nisin and the hydroxypropyl-beta-cyclodextrin, and the non-denatured type I collagen forms a continuous porous network structure. After the modified embedded nisin is applied to high-temperature baked egg processing, the total number of bacterial colonies of the baked eggs can be smaller than 103 CFU / g, the modified embedded nisin also has an excellent antibacterial effect on gram-negative bacteria of escherichia coli, the storage period reaches 3 months, and the shelf life is prolonged.

This invention belongs to the technical field of medical gels, specifically relating to a gynecological antibacterial gel and its preparation method. The gel comprises an antibacterial phase and a probiotic phase. Through the synergistic ratio of naringin, nisin, and lauroyl arginine ethyl hydrochloride, broad-spectrum inhibition of common pathogenic bacteria is achieved. Simultaneously, the introduction of *Lactobacillus rhamnosus* metabolites and sodium hyaluronate constructs a probiotic phase, promoting probiotic colonization and the restoration of vaginal microecological balance. The gel maintains a stable pH of 4.0–4.5, exhibiting good rheological properties and retention performance. In the preparation method, each component is added stepwise, and the system temperature and stirring conditions are controlled to ensure component activity and product stability. This invention can effectively alleviate bacterial vaginosis, fungal infections, and microecological disorders, providing synergistic, safe, and gentle comprehensive care effects.

The invention discloses a compound antibacterial sustained release preparation for gestational diabetes mellitus combined infection and a preparation method thereof, and relates to the technical field of medicines.The preparation method comprises the following steps that S1, a PLGA-PEG-esterase substrate copolymer, nisin and dipotassium glycyrrhizinate are jointly dissolved in an organic solvent through a nanoprecipitation method, and an organic phase is formed; injecting the organic phase into a water phase containing polyvinylpyrrolidone accounting for 0.5%-2% of the weight of the water phase at the shear rate of 5000-10000 rpm, and mixing; according to the compound antibacterial sustained-release preparation for gestational diabetes mellitus complicated with infection and the preparation method thereof, the poloxamer 407 / chitosan temperature-sensitive gel matrix enables the preparation to be a liquid which is easy to perfuse at room temperature, is convenient for administration, and is rapidly converted into a semi-solid gel at body temperature after entering the vagina, so that the residence time of the preparation in the vagina is prolonged, and long-acting sustained release is realized.

The invention discloses a preparation method of a composite biological fresh-keeping agent and application of the composite biological fresh-keeping agent in crab roe sauce, and belongs to the technical field of aquatic product processing and fresh-keeping. The green composite biological fresh-keeping agent comprises the following components: tea polyphenol, nisin, epsilon-polylysine hydrochloride and sterile water. Compared with a chemical preservative, the adopted green biological preservative better meets the requirements of consumers for food safety and health, contains multiple functional substances such as antioxidant components and antibacterial components, has the advantages of wide antibacterial spectrum, high safety and the like compared with a single preservative, can achieve the purposes of delaying oxidation of the crab spawn sauce and inhibiting growth of microorganisms, and is suitable for large-scale production. Therefore, the shelf life of the crab roe sauce is prolonged. The green composite biological fresh-keeping agent is applied to the crab spawn sauce, and by analyzing the change conditions of indexes such as microbial stability, protein degradation and lipid oxidation degree of the crab spawn sauce in the refrigeration process, the green composite biological fresh-keeping agent is proved to be capable of effectively inhibiting main putrefying bacteria in the refrigeration process of the crab spawn sauce, and has good bacteriostatic action and fresh-keeping effect.

The application discloses a kind of herbal bacteriostatic paste and preparation method thereof, wherein, herbal bacteriostatic paste, including the following mass fraction components: purple flower ground ivy extract-chitosan grafting: 3-8 parts;Dandelion flavone sulfonated derivative: 2-6 parts;Spilanthes acoca-tolerant streptococcus complex: 4-9 parts;Osthole-β-cyclodextrin inclusion compound: 1-5 parts;Matrix component: 30-60 parts;Cosolvent: 2-8 parts;Natural humectant: 5-15 parts;pH regulator: 0.1-1.5 parts;Deionized water: 10-30 parts;The present application adopts purple flower ground ivy extract-chitosan grafting, dandelion flavone sulfonated derivative, spilanthes acoca-tolerant streptococcus complex, osthole-β-cyclodextrin inclusion compound, by reasonable proportioning, realize synergistic bacteriostatic effect, four kinds of ingredients synergistic effect, broad antibacterial spectrum, staphylococcus aureus, escherichia coli, candida albicans and other common pathogenic bacteria have significant inhibitory effect, and not easy to produce drug resistance.

This utility model discloses a temperature control device for a fermenter used in the preparation of nisin. The device includes a fermenter covered by an insulated tank, with a cavity between the fermenter and the insulated tank filled with a temperature-controlled liquid. An impeller assembly is mounted on the upper part of the cavity and rotates outside the fermenter. A spiral cleaning plate is fixed to the side of the impeller assembly. This temperature control device regulates the temperature inside the fermenter by controlling the circulation of the temperature-controlled liquid outside the fermenter for heat exchange, facilitating the growth of *Streptococcus lactis*. During the circulation of the temperature-controlled liquid, the cleaning plate rotates outside the fermenter to clean scale, mucus, and other deposits, ensuring efficient heat exchange between the fermenter and the temperature-controlled liquid. The device also filters foreign matter from the temperature-controlled liquid, facilitating its reuse.

The invention relates to an engineering bacterium for producing nisin, and belongs to the field of synthetic biology. Specifically, the invention provides an engineering bacterium which can efficiently produce nisin and has a wide application prospect.