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A method for producing nisin by using cheap raw materials

A technology for nisin and nisin, which is applied in the field of nisin production to achieve the effects of comprehensive utilization of resources and reduction of production costs

Active Publication Date: 2016-08-03
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the production of nisin by fermenting molasses raw materials in industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Seed medium (per L): sucrose 10g, peptone 5g, yeast powder 5g, sodium chloride 2g,; dipotassium hydrogen phosphate 10g, magnesium sulfate 0.2g / L; use a triangular flask to cultivate seeds, the liquid volume is 200mL / 500mL . Cultivate in a shaking table at 30°C and 50rpm for 6h;

[0022] Fermentation medium (per L): 60g of sugarcane molasses (based on reducing sugar), 20g of bean cake, 2g of sodium chloride, 10g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, 801g of Tween;

[0023] Batch fermentation was carried out in a 10L fermenter with an inoculum size of 8%. During the fermentation process, the pH was controlled by feeding 20% ​​liquid caustic soda to about 6.5, the aeration culture was closed, the stirring speed was 80rpm, and the temperature was 30°C;

[0024] After 24 hours of shaker culture, the final titer of nisin was 5263IU / mL.

Embodiment 2

[0026] According to the method of Example 1, the primary seeds were first cultivated with shake flasks, and then the secondary seeds were cultivated with a 10L fermenter, and the seed cultivation time was about 5h. Adopt 100L fermenter to cultivate, begin to add a small amount of sugarcane molasses, control the initial reducing sugar concentration to be 15g / L, continuously add molasses during the fermentation process, control reducing sugar concentration to be 20-25g / L. The added amount of bean cake was 15g / L, and then other nutrients were added according to Example 1. The volume after sterilization was controlled to 75 L, and 5 L of seeds were transferred to it, the stirring speed was 50 rpm, the fermentation temperature was 30° C., and the culture was carried out in closed air. During the fermentation process, the pH was maintained at about 6.5 by feeding 20% ​​NaOH;

[0027] Adding molasses was stopped at 16 hours, and after 20 hours of fermentation, the amount of residual...

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PUM

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Abstract

The invention discloses a method for producing nisin by using cheap raw materials, which belongs to the technical field of microbial fermentation. The present invention uses Streptococcus lactis E-15 that was bred by mutagenesis, uses molasses as a carbon source, and soybean cake or soybean meal as a nitrogen source to ferment under anaerobic conditions, maintains pH 5.0-7.0, and performs batch or fed-batch fermentation 15-30h, the final fermented broth titer reaches 5000IU / mL. The outstanding advantage of the present invention is that cheap molasses and soybean meal are used as raw materials to produce nisin, which reduces the consumption of materials such as sucrose, yeast powder and peptone, and can reduce production costs.

Description

technical field [0001] The invention relates to a method for producing nisin by using molasses, soybean cake or soybean meal as raw materials, and belongs to the technical field of microbial fermentation. Background technique [0002] Nisin (groupNinhibitorysubstance), also known as nisin (peptide), nisin, is produced by nisin streptococcus ( Streptococcus lactis ) or Lactococcus lactis ( Lactococcus lactis ) A class of small molecule polypeptide compounds produced by fermentation of some strains. It can effectively inhibit the growth of Gram-positive bacteria and their spores that cause food spoilage, and prolong the shelf life of food. At the same time, Nisin is easily degraded by some proteases in the human digestive tract and will not accumulate in the human body. It is a safe natural preservative recognized worldwide. [0003] The production of Nisin is closely related to the growth of the strain. Studies have shown that the synthesis of Nisin occurs when the strain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C12R1/46
Inventor 康传利苏移山朱希强袁艳华张晓元凌沛学
Owner SHANDONG FREDA BIOTECH
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