Nisin compound and application thereof in preparation of antibacterial agent

A nisin and compound technology, applied in the field of antibacterial agents, can solve the problems of Nisin easy to unite and achieve the effects of improving antibacterial effect, high food safety, and improving economic benefits

Pending Publication Date: 2021-05-25
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a compound of nisin and monoglyceride and its application in the preparation of bacteriostatic agent, which can obviously improve the bacteriostatic effect,...

Method used

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  • Nisin compound and application thereof in preparation of antibacterial agent
  • Nisin compound and application thereof in preparation of antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Nisin complex

[0021] Weigh 2.0 grams of Nisin (purity 90%) produced by Tianjin Kangyi Biological Co., Ltd., add 150 mL of deionized water and stir to dissolve, add 8.0 grams of glyceryl monostearate (produced by Weifang Shengbaolong Biotechnology Co., Ltd.), and use 0.05 mol. L -1 Adjust the pH of the aqueous HCl solution to 5.0, put it in an ultrasonic instrument with 600W power for 5 minutes, insulate and mechanically stir at 25°C for 60 minutes, rotate and evaporate 120mL of water at a vacuum degree of 0.1atmospheric pressure at 60°C, and then vacuum dry at a vacuum degree of 0.1atmospheric pressure at 50°C to obtain Nisin complex 9.8g, water content 8.6%.

[0022] The nisin complex and nisin were dissolved in deionized water respectively to form a mass concentration of 0.1%. Taking deionized water as a reference, the absorption scan was carried out in the 200-250nm band of the ultraviolet spectrophotometer ( figure 1 with figure 2 ), the scanning curve of n...

Embodiment 2

[0035] 1. Nisin complex

[0036] Weigh 9.0 grams of Nisin (purity 80%) produced by Zhengzhou Chengwang Chemical Food Additive Co., Ltd., add 150 mL of deionized water and stir to dissolve, add 1.0 gram of glyceryl monostearate (produced by Weifang Shengbaolong Biotechnology Co., Ltd.), and use 0.05 mol L -1 Adjust the pH of the aqueous HCl solution to 4.0, put it in an ultrasonic instrument with 600W power and sonicate for 6 minutes, heat and mechanically stir at 25°C for 90 minutes, rotate and evaporate 120mL of water at a vacuum degree of 0.1atmospheric pressure at 60°C, and then vacuum dry at a vacuum degree of 0.1atmospheric pressure at 50°C to obtain Nisin complex 9.6g, water content 9.1%.

[0037] 2. Antibacterial test

[0038] As in Example 1, 2.0 grams of the nisin complex in the compound bactericidal test was dissolved in 180 mL of deionized water, and the amount of nisin in the nisin control was changed to 1.8 grams, and the monoglyceride control in the monoglyceri...

Embodiment 3

[0042] 1. Nisin complex

[0043] Weigh 7.0 grams of Nisin (purity 90%) produced by Wuhan Xianbao Biotechnology Co., Ltd., add 150 mL of deionized water and stir to dissolve, add 3.0 grams of glyceryl monostearate (produced by Weifang Shengbaolong Biotechnology Co., Ltd.), and use 0.05 mol · L -1Adjust the pH of the aqueous HCl solution to 6.0, put it in an ultrasonic instrument with 600W power and sonicate for 4 minutes, heat and mechanically stir at 25°C for 70 minutes, rotate and evaporate 120mL of water at a vacuum degree of 0.1atmospheric pressure at 60°C, and then vacuum dry at a vacuum degree of 0.1atmospheric pressure at 50°C to obtain Composition 9.7 g, water content 8.9%.

[0044] 2. Antibacterial test

[0045] Same as in Example 1, 2.0 grams of nisin complex in the compound bactericidal test was dissolved in 140 mL of deionized water, the amount of nisin in the nisin control was changed to 1.4 grams, and the monoglyceride control in the monoglyceride The fatty aci...

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Abstract

The invention discloses a nisin compound and application thereof in preparation of an antibacterial agent. The compound is prepared from nisin and monoglyceride in a mass ratio of 1:(0.1-5). The monoglyceride comprises glyceryl monostearate, glyceryl monopalmitate and glyceryl monooleate. According to the invention, monoglyceride and Nisin are compounded in water to obtain a compound of two substances, and high food safety can be maintained. Through compounding, the antibacterial effect can be obviously improved, the antibacterial effect on the tested gram-positive strain staphylococcus aureus and the compound is improved by 50% or above compared with that of Nisin with the same amount, and the antibacterial effect on the tested gram-negative strain escherichia coli and the compound is improved by 30% or above compared with that of Nisin with the same amount. Monoglyceride is a low-cost food raw material, and the compound also improves economic benefits.

Description

[0001] (1) Technical field [0002] The invention relates to an antibacterial agent, in particular to a nisin complex and its application in the preparation of an antibacterial agent. [0003] (2) Background technology [0004] Nisin, as a high-safety food preservative, has been approved for use in many countries around the world. Compared with other food preservatives, Nisin has higher safety and better antibacterial effect, so it has an ideal application prospect. In order to improve the antibacterial effect of Nisin, domestic and foreign scientific and technological personnel have carried out many explorations, but none of them have achieved an effective method for improving the antibacterial effect of Nisin. The invention adopts the compound of monoglyceride and Nisin, and the antibacterial effect of the compound on Gram-positive bacteria and negative bacteria is higher than that of Nisin. Monoglyceride is a food raw material, and the combination with Nisin does not affec...

Claims

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Application Information

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IPC IPC(8): A23L3/3535A23L3/3499
CPCA23L3/3535A23L3/3499A23V2002/00A23V2200/10A23V2250/192A23V2250/55
Inventor 钱俊青李新晨
Owner ZHEJIANG UNIV OF TECH
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