37 results about "Protamine sulfate" patented technology

The present invention discloses a method for quantification of 146s content in foot-and-mouth disease antigen by using a liquid chromatography detection system. The method comprises: adopting protamine sulfate to purify virus, adopting PEG6000 to concentrate the virus, carrying out sucrose density gradient ultracentrifugation on the concentrated virus, adopting a liquid chromatography detection system to detect an absorption peak of the centrifugated sample at a wavelength of 259 nm, and calculating 146S content according to the following formula: C=FRS/76Wb. The method can be used for determination of the 146S content in various types of foot-and-mouth disease antigens. With quantification of the 146S content in the foot-and-mouth disease antigen, foot-and-mouth disease vaccine preparation can be guided, vaccine efficacy can be indirectly evaluated, and major guiding significances are provided for enhancing quality of the foot-and-mouth disease vaccine in our country.

The invention relates to a method for producing batch hydrophobia vaccine, wherein it comprises that: (1), in the biological reactor with fixed bed mixing system and Fibra-Cel disks carrier, using cell increment cultivate liquid to cultivate Vero cell; (2), when the Vero cell grows to some density, using cell hold cultivate liquid, seeding hydrophobia vaccine, and affecting cell; (3), increasing virus; (4), obtaining virus continuously; (5), ultra-filter concentrating and inactivating via beta-propanolide; (6), using protamine sulfate or DNA enzyme treatment to remove Vero cell DNA; (7), using Sepharose 4 Fast Flow as stuff to process chromatography purification; (8), adding human blood albumin and sugar as protector to be made into liquid agent; or adding human blood albumin, sugar, and gelatin, as protector and shaping agent to be made into dried agent. The inventive method can produce virus continuously in small biological reactor, to realize batch production.

The present invention discloses a foot-and-mouth disease purification vaccine, a preparation method and applications thereof. The preparation method comprises: adopting protamine sulfate precipitation to remove most of hybridproteins and nucleic acids of host cells; condensing a virus liquid through a concentration system to be adopted as a chromatography sample; and selecting a polyacrylamide dextran gel as a chromatography medium to carry out purification elution, collecting a first elution peak, carrying out filtration sterilization inactivation, adding an adjuvant to carry out emulsification, and carrying out sub-packaging to obtain the foot-and-mouth disease purification vaccine. The preparation method has characteristics of economy, efficiency, low cost, simple and reasonable process, and effective removal of impurities in the host cells and the culture medium, wherein virus recovery rate is more than 85%, protein and nucleic acid removal rate is more than 90%, and the foot-and-mouth disease purification vaccine prepared by the preparation method has characteristics of safety, good immunity and stable property.

The invention discloses a broad-spectrum biological bactericidal air filtration material and a preparation method thereof. The invention is not only applicable to the general families and business departments with the need of air purification, but is also applicable to the semiconductor, pharmaceutical, hospitals and other environments with higher requirements of cleanliness. The broad-spectrum biological bactericidal air filtration material of the invention comprises at least one biological anti-bacterial agent and glass fiber filter paper, and the biological anti-bacterial agent is lysozyme, protamine sulfate, poly-lysine, nisin, polymyxin sulfate, glycine or natamycin. The preparation method of the invention is that the air filtration material is prepared by silanization, hydroformylation, condensation reaction and removal of the physically absorbed anti-bacterial agent. The biological bactericidal air filtration material prepared by the invention has low consumption of the anti-bacterial agent and no influence on the resistance of the filtration material; the sterilization rate is high, most of the killing rate of bacterial, spores and viruses is more than 99 percent, and the fungal growth is inhibited.

The present invention is double time phase protamine zinc insulin injection (30 %) and its preparation process, and belongs to the field of medicine preparation technology. The double time phase protamine zinc insulin injection (30 %) consists of insulin 100000 IU, isosmotic agent 35-45 g, preservative 5.5-7 g, pH regulator 12-12.5 g and fish protamine sulfate 0.21-0.42 g, and has pH value of 6.9-7.8. Its preparation process includes the steps of preparing neutral insulin injection, preparing low protamine zinc insulin injection, and mixing these two kinds of injection. The double time phase protamine zinc insulin injection (30 %) has two forms of insulin, including one in solution for quick acting and the other in precipitate with combined fish protamine sulfate as the middle acting phase. It has less required injection times.

The invention relates to a self-assembly nano adjuvant and a preparation method of a nano vaccine formed by the self-assembly nano adjuvant and application. The nano adjuvant comprises self-assembly materials of protamine sulfates and carboxymethyl glucan, the self-assembly materials and CpG oligodeoxynucleotides self-assembly form the nano adjuvant, and the nano vaccine is formed by the nano adjuvant and the virus antigen. The nano adjuvant increases the bioavailability of the CpG oligodeoxynucleotides, degradation in the body is avoided, and the B type CpG is added the function of A type CpG. The nano vaccine not only can induce the humoral immune response of TH1 type, but also induce relatively high cellular immune response. The unvaccinated experiments in mice body proves that the nanovaccine has good protection function, which provides help in the application of nano vaccine in the future.

Provided is a reagent for assaying a thrombin-antithrombin complex (TAT) in a blood sample from a subject by latex agglutination assay. The reagent includes a polycation. As a result, TAT complexes can be precisely assayed while circumventing the effect of heparin. The polycation is, for example, hexadimethrine bromide, chitosans, modified dextran, aminodextran, hydroxymethyl cellulose trimethylamine, lysozyme, spermine, spermidine, polylysine, polyarginine, polyornithine, protamine sulfate, hydroxyethyl cellulose trimethylamine, heparin-binding protein, polyallylamine, polyallylamine hydrochloride, poly(diallyl dialkyl amine), polyamideamine, polyamine, polyvinylbenzyltrimethylammonium chloride, polydiallyldimethylammonium chloride, polyethyleneimine, polypropyleneimine, polypropylethyleneimine, polyimidazoline, polyvinylamine, polyvinyl pyridine, poly(acrylamide/methacryloxypropyltrimethylammonium bromide), poly(diaryldimethylammonium chloride/N-isopropylacrylamide), poly(dimethylaminoethyl acrylate/acrylamide), poly(dimethylaminoethyl methacrylate), polydimethylaminoepichlorohydrin, polyethyleneiminoepichlorohydrin, polymethacryloxyethyl-trimethylammonium bromide, hydroxypropylmethacryloyloxyethyl dimethyl ammonium chloride, poly(methyldiethylaminoethylmethacrylate/acrylamide), poly(methyl/guanidine), polymethylvinylpyridinium bromide, poly(vinylpyrrolidone-dimethylaminoethyl methacrylate), or polyvinylmethylpyridinium bromide.

The invention discloses a blue light-emitting antibacterial carbon dot and a preparation method and application thereof. The preparation method comprises the steps that a carbon source is subjected toa heating reaction to prepare the carbon dot, wherein the carbon source comprises protamine sulfate. In the method, the protamine sulfate is specifically selected as a carbon source to be heated to prepare the carbon dots, the blue light-emitting carbon dots capable of imaging and inhibiting bacteria at the same time can be synthesized through a one-step method, and the preparation method is simple. In addition, the blue light-emitting carbon dot is good in water solubility, stable in photochemical property, low in cytotoxicity and good in blood compatibility. Therefore, the antibacterial carbon dot has a wide application prospect in the field of dual functions of bacterial therapy and imaging.

The invention discloses a method for refining protamine sulfate. According to the technical scheme adopted by the invention, the method comprises the following steps: performing enzymolysis on the protamine sulfate, performing quick protein liquid-phase separation to purify enzymatic hydrolysate, desalting a protamine high-salt solution to obtain high-purity protamine sulfate. A polypeptide mixture with average molecular weight of 1100Da, obtained by performing enzymolysis on the protamine with thermolysin has the capacity of neutralizing heparin; immune response caused after injection is remarkably lowered, and the toxic and side effects are relieved.

The present invention provides a healthy natural preservative for thick chili sauce products and a production process thereof, and relates to the technical field of food additives. The preservative is made from the following raw materials: protamine sulfate, chitosan, tea polyphenol extract, spice extract, Chinese medicinal herb extract, trans-Cinnamic acid, agar oligosaccharides, sour agents, ethanol and water. The produced preservative is healthy and pure natural, is a compound natural preservative combining plant and animal sources, is completely harmless to the human body, can effectively prolong the shelf life of the thick chili sauce products, is capable of environmental protection and sterilization, and has a strong antibacterial ability.

The invention relates to the technical field of biochemical detection, in particular to a reagent used for measuring pancreatic lipase in serum or plasma, and a preparation method and application thereof. The invention provides pancreatic lipase measurement reagent which comprises sodium dodecyl sulfate, protamine sulfate and sphingomyelin. Since the sodium dodecyl sulfate, the protamine sulfate and the sphingomyelin are added into the pancreatic lipase measurement reagent, the interference function of hepatic lipase, lipoprotein lipase and cholesterol esterase in the serum for pancreatic lipase measurement can be obviously lowered, nonspecific lipolysis reaction in the serum or the plasma in a reaction process of the pancreatic lipase measurement can be effectively inhibited, meanwhile, the catalytic activity of the pancreatic lipase can guarantee to be effectively performed, pancreatic lipase measurement specificity and accuracy is improved, and the false positive rate of pancreaticlipase detection is effectively lowered.

"Chinese pharmacopoeia" (2010 edition) regulates that protamine sulfate titer measurement should adopt bioassay methods. A titer of a sample is measured by comparing the clotting times of fresh rabbit bloods, pig plasmas, or rabbit plasmas which are individually added with a heparin standard substance (S) and the sample (T). The market price of rabbit plasma is high, one dose of rabbit plasma costs 10 yuan, and contains 0.5ml of rabbit plasma; and at least 60 doses are needed to measure a protamine sulfate sample. An ultraviolet spectrophotometer is adopted, the solution light-transmittance is measured under a light with a wavelength of 550 nm, then the protamine sulfate titer is measured, in the operation rabbit plasma is not needed, thus the cost is reduced, and moreover the operation is more convenient.