Preparation method and application of graphene oxide-protamine/sodium alginate compound

A technology of protamine and sodium alginate, which is applied in the fields of material synthesis and biomedicine, can solve the problems of stacking of lamellae and affect biological applications, and achieves the effects of uniform particle size distribution, easy mass production and broad application prospects.

Active Publication Date: 2017-10-24
山东省循证医学研究院有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] However, the unmodified GO solution is sensitive to the solution environment such as solute, pH, and ionic strength, and is prone to sheet stacking in physiological environments, which affects its biological applications.

Method used

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  • Preparation method and application of graphene oxide-protamine/sodium alginate compound
  • Preparation method and application of graphene oxide-protamine/sodium alginate compound
  • Preparation method and application of graphene oxide-protamine/sodium alginate compound

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Embodiment 1

[0028] (1) Break the graphene oxide aqueous dispersion into small-sized graphene oxide aqueous dispersions with a cell disruptor, power 400w, ultrasonic time for half an hour, and conduct in an ice-water bath; (2) Dissolve protamine sulfate in deionized water , to prepare a 1 mg / ml solution; (3) Under magnetic stirring, slowly add 1 mg / ml GO aqueous dispersion to the solution and stir for 30 minutes. The mass ratio of GO to protamine sulfate was 1:4. Then the mixture was filtered and washed three times with a microporous membrane, and dispersed in pure water to obtain a GO-protamine aqueous dispersion; (4) Sodium alginate was dissolved in deionized water to prepare a 1 mg / ml solution; (5 ) Under magnetic stirring, slowly add GO-protamine aqueous dispersion to sodium alginate solution and stir for 30 minutes. The mass ratio of protamine sulfate to sodium alginate is 1:1. Then the mixture was suction-filtered and washed three times with a 0.22 μm microporous membrane, and disp...

Embodiment 2

[0031] (1) The graphene oxide aqueous dispersion is broken into small-sized graphene oxide aqueous dispersions with a cell breaker, the power is 250w, the ultrasonic time is 2 hours, and the ice water bath is used; (2) Protamine sulfate is dissolved in deionized water , to make a 0.2 mg / ml solution; (3) Under magnetic stirring, slowly add 0.2 mg / ml GO aqueous dispersion to the solution and stir for 30 minutes. The mass ratio of GO to protamine sulfate was 1:10. Then the mixture was filtered and washed three times with a microporous membrane, and dispersed in pure water to obtain a GO-protamine aqueous dispersion; (4) Sodium alginate was dissolved in deionized water to make a 0.2 mg / ml solution; ( 5) Under magnetic stirring, slowly add GO-protamine aqueous dispersion to sodium alginate solution and stir for 30 minutes. The mass ratio of protamine sulfate to sodium alginate is 1:1. Then the mixture was suction-filtered and washed three times with a 0.22 μm microporous membrane...

Embodiment 3

[0034] (1) Use a cell breaker to crush the graphene oxide aqueous dispersion into small-sized graphene oxide aqueous dispersions with a power of 300w and ultrasonic time of 1 hour in an ice-water bath; (2) Dissolve protamine sulfate in deionized water , to make a 0.5 mg / ml solution; (3) Under magnetic stirring, slowly add 0.5 mg / ml GO aqueous dispersion to the solution and stir for 30 minutes. The mass ratio of GO to protamine sulfate was 1:1. Then the mixture was filtered and washed three times with a microporous membrane, and dispersed in pure water to obtain a GO-protamine aqueous dispersion; (4) Sodium alginate was dissolved in deionized water to prepare a 0.5 mg / ml solution; (5 ) Under magnetic stirring, slowly add GO-protamine aqueous dispersion to sodium alginate solution and stir for 30 minutes. The mass ratio of protamine sulfate to sodium alginate is 1:1. Then the mixture was suction-filtered and washed three times with a 0.22 μm microporous membrane, and dispersed...

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Abstract

The invention discloses a preparation method and application of a graphene oxide-protamine / sodium alginate compound and belongs to the technical field of material synthesis and biological medicines. According to the preparation method disclosed by the invention, small-size graphene oxide (GO) capable of loading a drug is used as an inner core and a shell layer is formed by protamine sulfate and sodium alginate in sequence by adopting a layer-by-layer self-assembly technology through non-covalent adsorption. Layer-by-layer self-assembled nano-carrier water has good dispersion performance and stability. The natural protamine sulfate and the sodium alginate are used as polyelectrolyte and the small-size graphene oxide is used as a core; all the materials have good biocompatibility; an anti-tumor drug is loaded in the core and has a great drug loading amount and good stability; the drug has long-period release performance and the toxic side effect of pure utilization of the drug is reduced.

Description

technical field [0001] The invention relates to a preparation method of a graphene oxide-protamine / sodium alginate composite material based on layer-by-layer self-assembly technology and its application in antitumor drug carriers, belonging to the technical fields of material synthesis and biomedicine. Background technique [0002] Tumor is one of the most important diseases threatening human health. Although traditional tumor treatments such as chemotherapy and radiotherapy have good therapeutic effects on primary tumors, they often have relatively high toxicity and side effects. Therefore, it is urgent to seek new, smarter and safer tumor treatment methods. With the development of science and technology, nanotechnology has received more and more attention. A large number of studies have shown that nanoparticles can effectively deliver therapeutic drugs to target tissues or cells, increase the drug's in vivo circulation time, and avoid drug degradation and inactivation. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/36A61K47/04A61K41/00A61P35/00C08L5/04C08L89/00C08K3/04
CPCA61K41/0052A61K47/02A61K47/36A61K47/42C08L5/04C08L89/00C08L2203/02C08K3/04
Inventor 谢萌刘彩虹徐远国张雅楠张峰雷海琳杨娜
Owner 山东省循证医学研究院有限公司
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