Stable antiheparin lipase detection kit

Abstract

The invention discloses an enzymatic colorimetric lipase detection kit. The invention prepares a stable antiheparin lipase detection kit. The stable antiheparin lipase detection kit is a liquid-type double-reagent diagnostic detection kit mainly comprising a reagent R1 and a reagent R2, wherein chloroform, glucan and protamine sulfate are introduced into the reagent R1 on the basis of the conventional formula, glucan and glycerol are introduced into the reagent R2, and qualitative change of the reagent properties are achieved through the newly introduced substances; after introduction of a newformula, the lipase detection kit can eliminate interference on a result in the presence of heparin and bilirubin, and through introduction of a glucan series and the glycerol, the stability of the reagents is greatly enhanced. According to the stable antiheparin lipase detection kit provided by the invention, the stability of the reagents is enhanced, the anti-interference ability of the reagents is greatly improved, and raw materials related in the reagents are conventional raw materials, so that a condition is created for mass collocation and production of the reagents.

Description

[0001] The invention prepares a stable anti-heparin lipase detection kit, which mainly relates to the field of clinical medical detection, in particular to the clinical detection of lipase content in human body. Background technique [0002] Lipase is one of the main enzymes for hydrolyzing oil. Generally speaking, natural oil is the main substrate of lipase. When lipase acts on oil, its main function is to hydrolyze the oil bond connected by fatty acid and glycerol in oil. This is different from other hydrolysis Enzymatic hydrolysis is different. The lipase catalysis system is a heterogeneous system. The catalysis of water-soluble enzymes occurs at the interface of water-insoluble substrates and water. The mechanism of catalysis at this interface is not very clear and cannot be simply explained by the Michaelis-Menten theory. Mechanism of reaction between enzyme and substrate. Some people put forward the hypothesis that lipase is localized on the oil-water interface, and put...

AI Technical Summary

Problems solved by technology

[0006] The invention prepares a stable anti-heparin lipase detection kit, which largely solves some problems exposed by similar products on the market, such as: poor reagent stability, weak anti-interference ability, and inability to detect heparin samples , the cost of reagents is high, etc.

Images