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Foot-and-mouth disease purification vaccine, preparation method and applications thereof

A technology for foot-and-mouth disease and foot-and-mouth disease, which is applied in the field of vaccine preparation, can solve the problems of unstable immune protection, undetectable neutralizing antibodies, and low effective antigen content, and achieve the effects of low cost, stable properties, and good immunity

Active Publication Date: 2013-03-27
内蒙古必威安泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are two main problems in the existing inactivated foot-and-mouth disease vaccines: 1. The immune protection is not stable, mainly manifested in: after immunization, neutralizing antibodies cannot be detected or the antibody level is very low, or the immunization period is short, only 2 to 3 2. Large immune side effects, mainly: the downstream production process is still the traditional method without purification treatment, there are some impurities and allergic substances in the culture medium and host cells in the vaccine, and the effective antigen content is low
There is no report on the purification of veterinary vaccines by chromatography technology at home and abroad

Method used

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  • Foot-and-mouth disease purification vaccine, preparation method and applications thereof
  • Foot-and-mouth disease purification vaccine, preparation method and applications thereof
  • Foot-and-mouth disease purification vaccine, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 bovine foot-and-mouth disease bivalent inactivated purified vaccine (ONXC strain+JSL strain)

[0033] 1 material

[0034]1.1 Vaccine strains: the virus strain used for the production of type O foot-and-mouth disease inactivated vaccine for this product is ONXC / 92 strain and the virus strain used for the production of Asian type I foot-and-mouth disease inactivated vaccine is Asia1 / JSL / GSZY / 06 strain, purchased from Lanzhou Veterinary Research Place.

[0035] 1.2 Chemical reagents: BHK21 cells were prepared and preserved according to existing conventional methods for this experiment; protamine sulfate (product number p4380), BEA, both products of sigma company; 206 adjuvant was purchased from Seppic company in France.

[0036] 1.3 Chromatographic column and gel medium: the gel medium packing sepphacryl S-300 and the empty column XK26 are all products of GE;

[0037] 1.4 Microporous filtration system: filter elements with a pore size of 5...

Embodiment 2

[0046] The preparation of embodiment 2 porcine foot-and-mouth disease O type inactivated purified vaccine (OR / 80 strain+O / ZK / 93-08 strain)

[0047] 1 material

[0048] 1.1 Vaccine strains: The foot-and-mouth disease virus used to manufacture this product is O-type foot-and-mouth disease inactivated vaccine production strain OR / 80 and O-type foot-and-mouth disease inactivated vaccine production strain O / ZK / 93-08 purchased from Lanzhou Veterinary Medicine graduate School.

[0049] 1.2 Chemical reagents: BHK21 cells were prepared and preserved according to existing conventional methods for this experiment; protamine sulfate (product number p4380), BEA, both products of sigma company; 206 adjuvant was purchased from Seppic company in France.

[0050] 1.3 Chromatographic column and gel medium: empty column XK26; gel medium packing: sepphacryl S-300 are all products of GE;

[0051] 1.4 Microporous filtration system: Hollow fiber columns with a pore size of 5 microns, 1 micron and ...

Embodiment 3

[0060] The preparation of embodiment 3 porcine foot-and-mouth disease O type inactivated purified vaccine (OR / 80 strain+O / ZK / 93-08 strain)

[0061] 1 material

[0062] 1.1 Vaccine strains: The foot-and-mouth disease virus used to manufacture this product is O-type foot-and-mouth disease inactivated vaccine production strain OR / 80 and O-type foot-and-mouth disease inactivated vaccine production strain O / ZK / 93-08 purchased from Lanzhou Veterinary Medicine graduate School.

[0063] 1.2 Chemical reagents: BHK21 cells were prepared and preserved according to existing conventional methods for this experiment; protamine sulfate (product number p4380), BEA, both products of sigma company; 206 adjuvant was purchased from Seppic Company of France; PEG6000 was purchased from AMRESCO Company.

[0064] 1.3 Chromatographic column and gel medium: empty column XK26; gel medium packing: sepphacryl S-300 are all products of GE;

[0065] 1.4 Microporous filtration system: Hollow fiber columns ...

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Abstract

The present invention discloses a foot-and-mouth disease purification vaccine, a preparation method and applications thereof. The preparation method comprises: adopting protamine sulfate precipitation to remove most of hybridproteins and nucleic acids of host cells; condensing a virus liquid through a concentration system to be adopted as a chromatography sample; and selecting a polyacrylamide dextran gel as a chromatography medium to carry out purification elution, collecting a first elution peak, carrying out filtration sterilization inactivation, adding an adjuvant to carry out emulsification, and carrying out sub-packaging to obtain the foot-and-mouth disease purification vaccine. The preparation method has characteristics of economy, efficiency, low cost, simple and reasonable process, and effective removal of impurities in the host cells and the culture medium, wherein virus recovery rate is more than 85%, protein and nucleic acid removal rate is more than 90%, and the foot-and-mouth disease purification vaccine prepared by the preparation method has characteristics of safety, good immunity and stable property.

Description

technical field [0001] The invention relates to a foot-and-mouth disease vaccine and a preparation method thereof, in particular to a preparation method of a purified foot-and-mouth disease vaccine and the purified foot-and-mouth disease vaccine obtained by the preparation method, and also relates to the application of the obtained foot-and-mouth disease vaccine in preparing medicines for preventing animal foot-and-mouth disease. It belongs to the field of vaccine preparation. Background technique [0002] With the improvement of the intensive breeding level of livestock, especially the increase in the number of dairy cows, slight side effects of vaccines can cause huge economic losses, such as abortion of pregnant cows, decline in milk production and milk quality of lactating cows, etc. , There is a huge demand for vaccines without any side effects in the market. [0003] There are two main problems in the existing inactivated foot-and-mouth disease vaccines: 1. The immune...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/135A61K9/107A61P31/14C12N7/02
Inventor 徐树兰张海艳李少英赵炳武辛俊宝张澍王钦富武华
Owner 内蒙古必威安泰生物科技有限公司
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