Process for producing nisin using plant cake dregs as raw material

A nisin and plant technology, applied in food science, food preparation, application, etc., can solve the problems of low nisin production and high raw material prices, and achieve the effects of increasing production, reducing production costs and increasing production

Inactive Publication Date: 2009-11-18
武汉麦可得生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this invention exists the relatively expensive raw material price, the shortcoming that nisin output is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare rapeseed cake meal into 8g / 100ml water suspension, stir and mix well, boil for 30min, then cool to maintain temperature at 50°C, adjust pH to 9.00 with 5mol / l sodium hydroxide, add while stirring to form rapeseed cake Meal weight 4% trypsin, during the enzymolysis process, use 5mol / l sodium hydroxide to maintain the pH of the enzymolysis solution at 9.00, and enzymatic hydrolysis for 8 hours; adjust the temperature of the enzymolysis solution to 35°C, the pH is 7.00, and add it into rapeseed while stirring Use 4% neutral protease by weight of the cake, use 5 mol / l sodium hydroxide to maintain the pH of the enzymolysis solution at 7.00, and perform enzymolysis for 4 hours to obtain the enzymolysis solution. Add 1g / 100ml of diatomaceous earth to the enzymatic hydrolysis solution, and filter through the plate frame to remove impurities, and the filtrate is the plant protein enzymatic hydrolysis solution. The yield of nitrogen content of the plant protein enzymatic ...

Embodiment 2

[0030] Prepare the soybean meal into a 12g / 100ml aqueous suspension, stir and mix, boil for 30min, cool and maintain the temperature at 50°C, adjust the pH to 8.50 with 5mol / l sodium hydroxide, add 4% of the weight of the cake while stirring Papain with a concentration of 1000000000000000 % substrate concentration of trypsin, maintain the pH of the enzymolysis solution at 8.00 with 5 mol / l sodium hydroxide, and perform enzymolysis for 4 hours to obtain the enzymolysis solution. The enzymolysis solution is added with 1% diatomaceous earth and filtered through a plate frame to remove impurities, and the filtrate is the plant protein enzymolysis solution. The yield of nitrogen content of the plant protein enzymatic hydrolyzate prepared by this process is 33%.

[0031] Add water to the prepared plant protein enzymatic hydrolyzate, glucose, diammonium hydrogen phosphate and yeast extract to prepare 3L of a mixed solution with a nitrogen content of 3g / 100ml, wherein the added amoun...

Embodiment 3

[0034] Purify and pulverize the soybean meal, prepare it into 8g / 100ml aqueous suspension, stir and mix well, boil for 30min, then cool to maintain the temperature at 50°C, adjust the pH to 9.00 with 5mol / L sodium hydroxide, add soybean while stirring The compound enzyme A of soybean meal weight 4% (compound enzyme A is alkaline protease and papain, is formed by the ratio of alkaline protease: papain=2: 1), maintains enzyme with 5mol / L sodium hydroxide in the enzymolysis process The pH of the solution was 9.00, and the enzymolysis was carried out for 8 hours; the temperature of the enzymolysis solution was adjusted to 35° C., and the pH was 7.00, while stirring, the compound enzyme B (compound enzyme B was neutral protease and trypsin, obtained by adding 4% of soybean meal weight) was added. protease: trypsin = 1:1 ratio), use 5 mol / l sodium hydroxide to maintain the pH of the enzymolysis solution at 7.00, and enzymolysis for 4 hours to obtain the enzymolysis solution. Add 1g / ...

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Abstract

The present invention relates to biological antibiotic food additive producing technology, and is especially process of producing. The present invention features that lactic streptocin with plant cake dregs as raw material, and through the steps of enzymolysis, fermentation, solid-liquid separation, concentration, salting out, drying and packing. The technological process of the present invention has greatly raised lactic streptocin yield and lowered production cost.

Description

technical field [0001] The invention relates to a production technology of biological antibiotic food additives, in particular to a process for producing nisin by utilizing plant cake. Background technique [0002] Nisin is a fermentation metabolite of Lactococcus lactis, which can effectively inhibit the growth and reproduction of Gram-positive bacteria and their spores. It is a small molecular polypeptide substance composed of 34 amino acid residues. After entering the human digestive tract, it can be quickly decomposed by the protease in the human digestive tract, so that it can be absorbed and utilized by the human body. Therefore, it is a safe and non-toxic food. Preservatives, and will not affect the beneficial bacteria in the intestines, and will not produce drug resistance. Nisin exists naturally in dairy products and is one of the few recognized natural and non-toxic biological antibiotic food preservatives. my country stipulates that it can be used as a food prese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02A23L1/305A23L33/18
Inventor 陈守文岳传静鲁伟
Owner 武汉麦可得生物技术有限公司
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