Method for producing nisin by using cheap raw materials

A technology of nisin and nisin, applied in the field of nisin production, to achieve the effect of reducing production costs and comprehensively utilizing resources

Active Publication Date: 2014-05-21
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the production of nis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Seed medium (per L): 10g sucrose, 5g peptone, 5g yeast powder, 2g sodium chloride, 10g dipotassium hydrogen phosphate, 0.2g / L magnesium sulfate; cultivate seeds in a triangular flask with a liquid volume of 200 mL / L 500mL. Cultivate in a shaking table at 30°C and 50rpm for 6h;

[0022] Fermentation medium (per L): 60g of sugarcane molasses (based on reducing sugar), 20g of bean cake, 2g of sodium chloride, 10g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, 1g of Tween-80;

[0023] Batch fermentation was carried out in a 10L fermenter with an inoculum size of 8%. During the fermentation process, the pH was controlled by feeding 20% ​​liquid caustic soda to about 6.5, the aeration culture was closed, the stirring speed was 80rpm, and the temperature was 30°C;

[0024] After 24 hours of shaker culture, the final titer of nisin was 5263IU / mL.

Embodiment 2

[0026] According to the method of Example 1, the primary seeds were first cultivated with shake flasks, and then the secondary seeds were cultivated with a 10L fermenter, and the seed cultivation time was about 5h. Adopt 100L fermenter to cultivate, begin to add a small amount of sugarcane molasses, control the initial reducing sugar concentration to be 15g / L, continuously add molasses during the fermentation process, control reducing sugar concentration to be 20-25g / L. The added amount of bean cake was 15g / L, and then other nutrients were added according to Example 1. The volume after sterilization was controlled to 75 L, and 5 L of seeds were transferred to it, the stirring speed was 50 rpm, the fermentation temperature was 30° C., and the culture was carried out in closed air. During the fermentation process, the pH was maintained at about 6.5 by feeding 20% ​​NaOH;

[0027] Adding molasses was stopped at 16 hours, and after 20 hours of fermentation, the amount of residual...

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Abstract

The invention provides a method for producing nisin by using cheap raw materials, belonging to the technical field of microbial fermentation. The method comprises the following steps: carrying out fermentation under anaerobic conditions, wherein lactic streptococci E-15 obtained through mutation breeding is employed, molasses is used as a carbon source, and a bean cake or bean pulp is used as a nitrogen source; maintaining a pH value to be 5.0 to 7.0; and carrying out batch fermentation or fed batch fermentation for 15 to 30 h, wherein the titer of final fermentation broth reaches 50000 IU/mL. The method provided by the invention has the following protruding advantage: the cheap molasses and bean cake/pulp are used as the raw materials for production of nisin, the usage amount of materials like cane sugar, yeast powder and peptone is reduced, so production cost is reduced.

Description

technical field [0001] The invention relates to a method for producing nisin by using molasses, soybean cake or soybean meal as raw materials, and belongs to the technical field of microbial fermentation. Background technique [0002] Nisin (group N inhibitory substance), also known as nisin (peptide), nisin, is produced by nisin ( Streptococcus lactis ) or Lactococcus lactis ( Lactococcus lactis ) A class of small molecule polypeptide compounds produced by fermentation of some strains. It can effectively inhibit the growth of Gram-positive bacteria and their spores that cause food spoilage, and prolong the shelf life of food. At the same time, Nisin is easily degraded by some proteases in the human digestive tract and will not accumulate in the human body. It is a safe natural preservative recognized worldwide. [0003] The production of Nisin is closely related to the growth of the strain. Studies have shown that the synthesis of Nisin occurs when the strain enters the...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/46
Inventor 康传利苏移山朱希强袁艳华张晓元凌沛学
Owner SHANDONG FREDA BIOTECH
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