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Biological preservative as well as preparation method and application thereof

A biological antiseptic and antistaling agent technology, applied in the fields of application, food science, food preservation, etc., can solve the problems of drug resistance of bacteria, environmental pollution, pesticide residues, etc., and achieve the effect of scientific conception, broad application prospects, and low cost

Inactive Publication Date: 2011-09-07
EAST CHINA NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The biological antiseptic and antistaling agent of the present invention overcomes the problems of environmental pollution, pesticide residues and drug resistance to germs caused by the use of chemical bactericides in the prior art

Method used

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  • Biological preservative as well as preparation method and application thereof
  • Biological preservative as well as preparation method and application thereof
  • Biological preservative as well as preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0032] The preparation steps of biological preservative of the present invention are as follows:

[0033] Step ①: Cultivate Propionibacterium in a medium containing 10-20% whey powder, 5-10% yeast extract, and 0.01-0.2% inorganic salt for 100-120 hours.

[0034] Step ②: Collect the fermentation broth in step ①, and obtain the propionibacterium metabolites through centrifugation, ultrafiltration, and vacuum concentration.

[0035] Step ③: Spray-dry / freeze-dry the metabolites of Propionibacterium in step ② to obtain dry powder of metabolites of Propionibacterium.

[0036] Step ④: Add the dry powder of propionibacterium metabolites obtained in step ③ and nisin into the solvent in a certain proportion, and prepare suspensions with different concentrations to obtain the antiseptic and fresh-keeping agent of the present invention.

[0037] The biological antiseptic and antistaling agent of the present invention contains 70-80% of propionibacterium metabolites and 20-30% of nisin. ...

Embodiment 1

[0038] Embodiment 1 The biological preservative of the present invention is used for antisepsis and preservation of strawberries

[0039] Strawberries are sorted and graded—cleaned and drained of water—naturally dried—sprayed with different concentrations of biological preservatives—naturally dried—observed and recorded (temperature 16—22°C, humidity 58%—65%).

[0040]The preparation of the biological antiseptic preservative of the present invention: add the dry powder of propionibacterium metabolites and nisin into the solvent at a ratio of 4:1, and prepare a series of suspensions with a concentration of 2%-4%, respectively. (CK is the blank group sprayed with distilled water, sprayed wet and then dried, 2% is 1 group, 3% is 2 groups, 4% is 3 groups).

[0041] Regularly measure the change of strawberry weight, refer to GB / 8858-1988. To calculate the weight loss rate, the weight loss rate is expressed as a percentage of weight loss based on the average weight of fresh fruit. ...

Embodiment 2

[0060] Embodiment 2 The biological preservative of the present invention is used for the preservation and preservation of grape juice

[0061] Wash the fresh Kyoho grapes, peel and remove the seeds, put them into the juicer and stir at high speed, remove the dregs to get the first juice, and centrifuge the first juice at 3000rpm for 10min. The centrifuged juice removes the upper impurities and sediment, which is what you want Grape juice, pH natural (between 3.3-4.0). The obtained grape juice is pasteurized and packed into threaded test tubes for later use.

[0062] The preparation of the biological antiseptic and antistaling agent of the present invention: mix the dry powder of propionibacterium metabolites and nisin in a ratio of 7:3 evenly. Add 1‰, 5‰, 1% and 5% to the prepared fruit juice respectively, take the fruit juice without adding the biological preservative of the present invention as the blank control, put the fruit juice into the culture at 4°C or 25°C , and ta...

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Abstract

The invention provides a novel biological preservative which contains 70-80% of Propionibacterium thoenii metabolite and 20-30% of Nisin. The invention further provides a preparation method and application of the preservative. In the preservative, the Propionibacterium thoenii antibacterial metabolite can be used for inhibiting Gram-negative bacteria, mould and yeast, and the Nisin can be used for inhibiting Gram-positive bacteria. The preservative provided by the invention has the advantages of being safe and harmless to a human body and good in broad-spectrum antibacterial and antiseptic effect; and preparation method has the advantages of simplicity, lower cost and wide application prospect.

Description

technical field [0001] The invention relates to the field of food technology, in particular to a biological antiseptic and preservative comprising metabolites of Propionibacterium terreii and nisin, a preparation method and application thereof. Background technique [0002] As the saying goes, "Without food additives, there would be no modern food industry." One of the most effective ways to extend the shelf life of food and reduce the occurrence of people's microbial food poisoning is to add food preservatives. At present, although chemically synthesized preservatives commonly used in the food industry have good antiseptic effects, they have potential carcinogenicity, teratogenicity, and food poisoning. According to a report recently released by the American Health Foundation and the National Cancer Institute, about 50% of the 5 million people who suffer from cancer worldwide are related to food contamination, and about 3% of them suffer from food preservation. agent. ...

Claims

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Application Information

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IPC IPC(8): A23L3/3571
Inventor 孙帅高红亮常忠义牛延宁步国建韦妮
Owner EAST CHINA NORMAL UNIV
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