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Method for evaluating toxicity of waste water on water treatment microorganisms

A microbial and water treatment technology, which is applied in the field of toxicity assessment of industrial wastewater, wastewater treatment or enhanced pretreatment, and the evaluation of wastewater to water treatment microbial toxicity. It can solve the high requirements of pre-aeration degree and sludge concentration. , time-consuming and other problems, to achieve the effect of simple method, simple operation steps and mild operating conditions

Inactive Publication Date: 2018-08-28
HUATIAN ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can evaluate the biotoxicity of wastewater to activated sludge, but it takes a long time and has high requirements on the degree of pre-aeration and sludge concentration

Method used

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  • Method for evaluating toxicity of waste water on water treatment microorganisms
  • Method for evaluating toxicity of waste water on water treatment microorganisms
  • Method for evaluating toxicity of waste water on water treatment microorganisms

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0047] The preparation of test bacterial liquid among the present invention:

[0048] 1) Collect the activated sludge from the aerobic biological treatment section from the sewage / wastewater treatment plant in normal operation. After the sludge is retrieved, it is fully dispersed and mixed;

[0049] 2) Use sterilized physiological saline for stepwise dilution. Shake and mix well during the dilution process. Inoculate the sludge with different dilution ratios on the plate, spread evenly, and incubate in a constant temperature incubator at 20°C for 16-18h.

[0050] 3) When starting the experiment, the microorganisms on a group of plates with 30-300 colonies were washed with sterile water, mixed evenly, inoculated in TSB medium, and prepared as a test bacterial solution. The absorbance of the test bacteria solution at the wavelength of 450-650nm reaches 0.3-0.7.

[0051] or,

[0052] 1) Take out the activated sludge microorganisms stored in the freezer (-80°C), melt them in a r...

Embodiment 1

[0064] Toxicity evaluation of mixed wastewater from a pharmaceutical factory to water treatment microorganisms

[0065] Select the mixed wastewater W of a pharmaceutical factory to evaluate its microbial toxicity. The determination steps are as follows:

[0066] (1) Filter the waste water W, combine with the amount of domestic sewage, and dilute it by three times (1, 1.2, 3) with a phosphate buffer solution (pH7.8);

[0067] (2) prepare the test bacteria liquid whose absorbance at the wavelength of 530nm reaches 0.3-0.7;

[0068] 21) Collect the activated sludge from the aerobic biological treatment section from the sewage / wastewater treatment plant in normal operation. After the sludge is retrieved, fully disperse and mix;

[0069] 22) Use sterilized physiological saline for stepwise dilution. During the dilution process, shake and mix well. Inoculate the sludge with different dilution ratios on the plate, spread evenly, and incubate in a constant temperature incubator at 20...

Embodiment 2

[0077] Toxicity assessment of wastewater from various production workshops of a pharmaceutical factory to water treatment microorganisms

[0078] A pharmaceutical factory has 5 main drainage workshops. The amount and quality of wastewater discharged from each workshop is different. The pharmaceutical factory needs to treat it to meet the discharge standard and discharge it to the urban sewage treatment plant. Assess the microbial toxicity of each waste water to determine whether enhanced pretreatment is required before biochemical treatment. The steps are as follows:

[0079] (1) Filter several strands of wastewater (A1, A2, A3, A4, A5), combine with the amount of domestic sewage, and dilute them with phosphate buffer solution (pH7.8) by three times (among them, the middle multiple is the wastewater as a percentage of the total volume of water);

[0080] (2) prepare the test bacteria liquid whose absorbance at the wavelength of 530nm reaches 0.3-0.7;

[0081] 21) Collect th...

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Abstract

The invention discloses a method for evaluating toxicity of waste water on water treatment microorganisms. The method comprises the following steps that 1, a water treatment microorganism test bacteria solution is prepared; 2, to-be-tested waste water is diluted into multiple parts, and dilution ratios of the parts are different; 3, diluted waste water is added in the prepared water treatment microorganism test bacteria solution to be mixed to be uniform; 4, the amount of the microorganisms in each part of the mixed liquid obtained after uniform mixing is measured regularly or in real time inpreset time so as to obtain a microorganism growth curve of each part of mixed liquid; 5, the measured microorganism growth curve is compared with a blank control. The method has universality, biotoxicity of waste water of different types can be evaluated, and by means of the detection results of the biotoxicity of the waste water with different sources and dilution ratios, waste water separate treatment and (enhanced) pretreatment can be guided.

Description

technical field [0001] The invention belongs to the field of water pollution control, in particular to a method for evaluating the toxicity of waste water to water treatment microorganisms, which is suitable for the evaluation of the toxicity of industrial waste water and waste water quality separation treatment or enhanced pretreatment. Background technique [0002] The treatment and zero discharge of industrial wastewater is a recognized problem in the domestic and foreign sewage treatment circles. Most of the sewage treatment process is based on biological treatment, but some of the wastewater discharged by enterprises or industrial park treatment stations contains toxic and harmful pollutants, which are often toxic to microorganisms, inhibit or kill them, resulting in poor biological treatment effects. The composition of industrial wastewater is complex. If the types and concentrations of pollutants in the wastewater are analyzed and detected, and then the toxic properti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
CPCG01N33/1866
Inventor 张红程寒飞詹茂华
Owner HUATIAN ENG & TECH CORP MCC
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