Multi-channel planar waveguide evanescent wave biosensor

A biosensor and planar waveguide technology, which is used in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of inability to measure multiple indicators at the same time, occupying a large space, and measuring a long period of time.

Inactive Publication Date: 2013-08-14
TSINGHUA UNIV +1
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Problems solved by technology

The disadvantages of this technology are: 1) The lens is used to collect fluorescence and occupies a large space, so the system can only accommodate one collection optical path. When measuring the fluorescence intensity of 4 sample cells, a stepping motor is required to move the sample cells together with the sensing elements sequentially Go to the next measurement position, s

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  • Multi-channel planar waveguide evanescent wave biosensor

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Embodiment Construction

[0014] The working process of the multi-channel planar waveguide evanescent wave biosensor of the present invention is as follows: first, the surface of the sensing element 4 is modified with various biorecognition molecules, and then the sensing element is combined with the sample pool 5 . Turn on the peristaltic pump 6, transfer the mixed sample of the analyte into the sample pool 5, after a certain period of reaction, the fluorescently labeled analyte is fixed on different parts of the sensing element; turn on the laser 1, and the excitation light is coupled into the sensing element 4. Evanescent waves are generated at each total reflection point to excite the fluorescent molecules fixed on the surface of the sensing element 4 to generate fluorescence; the fluorescence is collected by the multimode optical fiber group 3 and filtered by the filter group 8 before entering the photoelectric Detector group 9, the fluorescent signals of different parts on the surface of the sensi...

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Abstract

The invention provides a multi-channel planar waveguide evanescent wave biosensor, belongs to the technical field of biological testing, and particularly relates to fluorescent dye-labeled biological substance luminescence excited by laser, thereby achieving the biological detection technology. The biosensor is characterized in that: in the process of laser optical waveguide in a flat glass medium, a plurality of total reflection points are formed on the interface of two-phase medium, fluorescent labeled biological molecules in the point spatial range are excited by evanescent waves generated at the total reflection points to generate fluorescence, the fluorescence is collected by multimode fibers, and fluorescence signals are detected by a lock-in amplifier. As the laser in planar waveguide process can form a plurality of total reflection points, each total reflection point is marked with different biological molecules and the fluorescence signal is detected separately, simultaneous determination of multi-index in a sample can be achieved. The biosensor has the characteristics of simple waveguide structure, high fluorescence collection efficiency, small background noise interference, enabling simultaneous determination of multi-index and the like.

Description

technical field [0001] The invention designs a planar waveguide evanescent wave biosensor based on the principle of total internal reflection fluorescence, and its application fields include biomedicine, environmental monitoring, food inspection and the like. It can detect a variety of target substances such as biological macromolecules, small organic molecules, viruses and bacteria. Background technique [0002] When the light wave is transmitted in the glass medium by total internal reflection, an evanescent wave can be generated on the side of the optically sparse medium at the total reflection point. The evanescent wave is a kind of energy wave, and its transmission depth is usually only tens to hundreds Nano. In the space where the evanescent wave exists, the biological substances labeled with fluorescent molecules connected to the surface of the chip by adsorption or bioaffinity reaction are excited and generate fluorescence. The fluorescence intensity has a quantitat...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 施汉昌宋保栋何苗
Owner TSINGHUA UNIV
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