A method for developing markers by high-throughput sequencing of tetraploid potato and its application

Abstract

The invention discloses a method for developing a marker through tetraploid potato high-throughput sequencing and an application of the method. The method for developing the marker through the tetraploid potato high-throughput sequencing disclosed by the invention can greatly reduce the complexity of a potato genome and can facilitate operation; and by virtue of the method, high-density SNP (single nucleotide polymorphism) sites can be rapidly identified, a chromosome label difference genetic interval for controlling target character can be obtained, and finally, the molecular marker can be developed on the basis of the difference genetic interval. With the application of the method disclosed by the invention, the application of an 2b-RAD sequencing technology in potato tetraploid material marker development is successfully achieved; the difficulty on developing the potato tetraploid material marker is solved, and meanwhile, a development cycle of the character closely linked marker is greatly shortened. In addition, the method disclosed by the invention also provides a reference for genetic interval mining, marker development and genome research of other tetraploid organisms having complex genomes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for developing markers by high-throughput sequencing of tetraploid potatoes and an application thereof. Background technique [0002] Tetraploid potato (2n=4x=48) is highly heterozygous, has a high frequency of genetic recombination, and suffers from serious decline after selfing for many generations, but the genetic background is narrow, the gene pool is poor, and the development of molecular markers is difficult. Therefore, the potato genetic map has poor saturation and a small number of molecular markers, and is far behind in many aspects compared with rice, corn, soybean and other crops. The current genetic map mostly uses traditional markers such as SSR and AFLP, and marker development and genetic map construction are mainly carried out at the diploid level, and there are few reports on the development of markers directly at the tetraploid level. So far, th...

AI Technical Summary

Problems solved by technology

[0002] Tetraploid potato (2n=4x=48) is highly heterozygous, has a high frequency of genetic recombination, and suffers from severe recession after selfing for many generations, but has a narrow genetic background, poor gene pool, and difficult development of molecular markers

The current genetic map mostly uses traditional markers such as SSR and AFLP, and marker development and genetic map construction are mainly carried out at the diploid level, and there are few reports on the direct development of markers at the tetraploid level

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