Cultivation method for mulberry chromosome doubling

A chromosome doubling and breeding method technology, applied in the fields of botany equipment and methods, plant genetic improvement, horticulture, etc., can solve the problems of separation, the lack of timely mutation of organs, the physiological toxicity of colchicine, etc., and achieve the polyploid induction rate. high effect

Inactive Publication Date: 2016-10-12
NORTHWEST A & F UNIV
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Problems solved by technology

One of the main reasons is that colchicine is physiologically toxic, and improper handling results in the death of the treated individual; the second is that the action time of the drug is not enough; the third is that the mutant organs are not separated from the mother in time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Use 0.4%, 0.3%, 0.2%, and 0.1% colchicine with a concentration gradient from high to low to instill the shoot tips of the target parent's grafted seedlings for 5 consecutive days. 20~30cm;

[0012] (2) Use kraft paper bags or envelopes for moisturizing and shading during processing;

[0013] (3) After treatment, clean up the anvil buds sprouting below the treatment site in time;

[0014] (4) In the following year, single buds were separated and grafted on the branch segments of the abnormal leaves.

[0015] Experiments have proved that the chromosome doubling rate obtained by the grafted individual in this embodiment can reach 32-56%, with an average of 47%.

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Abstract

A method for cultivating mulberry chromosome doubling, comprising the following steps: (1) instilling 0.4 to 0.1% colchicine with a concentration gradient from high to low to the shoot tip of the target parent's grafted seedlings for 5 consecutive days , wherein the height of grafted seedlings is 20-30cm; (2) use kraft paper bags or envelopes for moisturizing and shading during the treatment; (3) promptly clean up the root buds germinated below the treatment site after treatment; Carry out single-bud separation grafting. The invention has the characteristics of high polyploid induction rate. Experiments have proved that the induction rate of polyploidy obtained by grafting individuals by adopting the breeding method of mulberry chromosome doubling of the present invention can reach 32-56%, and the average reaches 47%, which is far higher than the level at home and abroad.

Description

Technical field [0001] The invention relates to a method for cultivating mulberry artificial polyploid, in particular to a method for cultivating mulberry chromosome doubling. Background technique [0002] Mulberry polyploid has the advantages of vigorous growth, excellent leaf quality, and enhanced stress resistance. It is the main direction of mulberry breeding. At present, the common method for cultivating artificial polyploid mulberry is to use colchicine as a mutagen to treat mulberry seeds or growth buds. However, for a long time, the polyploid mutagenesis rate (the success rate of obtaining polyploidy) has been below 10% . One of the main reasons is that colchicine is physiologically toxic, and improper handling causes death of the treated individual; the second is the lack of time for the drug; and the third is that the mutant organs are not separated from the mother in time. Summary of the invention [0003] The technical problem to be solved by the present invention is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01G1/06
CPCA01H1/08A01G2/30
Inventor 苏超焦锋陈旗钱永华薛忠民肖乃康
Owner NORTHWEST A & F UNIV
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