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Induction method for hybrid orchid polyploidy

A technology of polyploid and hybrid orchids, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as difficult to obtain homogeneous polyploids, asynchronous cell division, easy formation of chimeras, etc., to achieve High application value, significant polyploid characteristics, and the effect of shortening the cycle

Inactive Publication Date: 2015-01-14
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

The traditional induction method for cultivating plant polyploids is to induce plant growth points, lateral buds or seeds, etc., and the buds composed of multi-cells develop into plants. Due to asynchronous cell division, it is easy to form chimeras, and it is difficult to obtain homogeneous polyploids. ploidy, the induction rate is also low

Method used

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  • Induction method for hybrid orchid polyploidy
  • Induction method for hybrid orchid polyploidy
  • Induction method for hybrid orchid polyploidy

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1) Take Cymbidium 'Eros' ( Cymbidium Lucky Gloria 'Aguri') and Tiger Xuelan[ Cymbidium hybridium × ( Cymbidium tracyanum varhuanghua x Cymbidium mastersii )] mature self-fertilized capsules that have not yet cracked, wipe them clean with 75% alcohol, disinfect them with 75% alcohol on the ultra-clean workbench for 3-6 minutes, wash them twice with sterile water, and then put them in 0.2 % mercuric chloride for 10-15 minutes, washed with sterile water 4 times, cut open the peel, and took out the seeds;

[0029] 2) Sow the seeds into 1 / 2MS basic medium, 0.5-2.5 mg / L6-BA, 0.1-1.0 mg / LNAA, 30mg / L sucrose and 6.0-7.0g.L -1 In the culture medium of agar, put it into a culture room with a temperature of 25-30°C for dark culture;

[0030] 3) Soak the aseptic spherical bulbs formed by the germination of hybrids in 3% colchicine solution, treat for 72 hours, rinse with sterile water 4-5 times, filter the water, transfer to 1 / 2MS+1.0mg / In the medium of L6-BA+0.5mg / LNAA+0...

Embodiment 2

[0036] The specific steps are as described in Example 1, the difference is that the colchicine solution in step 3 is directly added to the culture medium, the concentration is 3%, the treatment time is 14 days, and the variation rate is 26.7%.

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Abstract

The invention in particular relates to an induction method for hybrid orchid polyploidy belonging to the technical field of polyploidy breeding. The induction method for the hybrid orchid polyploidy comprises the following steps: taking sterile spherical stems formed by germination of hybrid seeds of Cymbidium Lucky Gloria 'Aguri' and Cymbidiumhybridium*(Cymbidiumtracyanumvarhuanghua*Cymbidiummastersii as test materials, carrying out polyploidy induction on F1-generation spherical stems by colchicines, then differentiating the induced spherical stems into buds and cluster buds, then carrying out propagation culture, carrying out rooting culture on plants which are identified as polyploidy to obtain polyploidy plant tissue culture seedlings. The hybrid orchid polyploidy plants obtained by combination of the spherical stems and a colchicines soaking method have remarkable polyploidy characteristics; the spherical stems are used as the induction materials, the period of breeding the polyploidy hybrid orchid plants is shortened; and the method is simple and easy to control.

Description

technical field [0001] The invention specifically relates to a polyploid induction method for hybrid orchids, and belongs to the technical field of polyploid breeding. Background technique [0002] Orchid is a precious flower with graceful posture and rich fragrance, and it is one of the ten famous traditional Chinese flowers. As one of the important commercial potted flowers and cut flowers, Cymbidium is currently favored by people. The common species of Cymbidium ‘Eros’ ( Cymbidium Lucky Gloria 'Aguri') is a perennial herbaceous plant, each plant has 1-5 scapes, the scapes are oblique, slightly curved, basically pulled out from the leaves, 35-80cm long; 5-10 flowers, flowers Large, bright red, flowering period from November to March of the following year, the flowering period is longer; 'Tiger Xuelan'[ Cymbidium hybridium × ( Cymbidium tracyanum varhuanghua x Cymbidium mastersii ] is bred by crossing the yellow pigment flower of Agarwood tiger head orchid and Snowl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王玉英李枝林李光宏孟英李志敏王亚沉
Owner YUNNAN AGRICULTURAL UNIVERSITY
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