A kind of cultivation method of white and polyploid plants

A breeding method and polyploid technology, which is applied in the field of cultivating white and polyploid plants, can solve the problem of high content of medicinal active ingredients, and achieve the effects of simple preparation method, significant polyploid characteristics, and high application value

Active Publication Date: 2014-10-29
ZHEJIANG CHINESE MEDICAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] 3. High content of medicinal active ingredients

Method used

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  • A kind of cultivation method of white and polyploid plants
  • A kind of cultivation method of white and polyploid plants
  • A kind of cultivation method of white and polyploid plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Take a Baiji capsule, soak it in washing powder for 10 minutes, and rinse it with tap water for 5 minutes. Then disinfect with 75% ethanol for 2 minutes, rinse with sterile water, disinfect with 0.1% mercury liter for 15 minutes, rinse the residual mercury with sterile water, and dry the remaining water on the surface of the capsule with sterile absorbent paper;

[0047] 2) Cut off the capsules of Baiji, take the seeds in the capsules and put them into 0.1% colchicine solution (with 1 / 2MS as the solvent), and cultivate them in the dark for 7 days. After the treatment is completed, centrifuge at 1000rpm for 1min to precipitate the seeds, take the seeds, wash them three times with sterile distilled water, and obtain the preliminary germinated seeds;

[0048] 3) Transfer the above-mentioned colchicine-treated Baiji seeds to the medium of MS+0.2mg / L1-naphthylacetic acid, place them in the incubator for dark culture for 5 days, and then move them to the culture room. The ...

Embodiment 2

[0056] The specific steps are as described in Example 1, except that the concentration of colchicine in step 2 is 0.20%, the treatment time is 9 days, and the variation rate is 41.07±1.34%.

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Abstract

The invention provides a method for cultivating rhizoma bletillae polyploidy plants; the method comprises the steps of using colchicine to treat rhizoma bletillae seeds, carrying out germination cultivation of the rhizoma bletillae seeds, carrying out enrichment cultivation on rhizoma bletillae seedlings and the like; and after the seedlings are identified to be polyploidy plants, transferring the seedlings into a rooting and strengthening culture medium for culturing to obtain the seedlings of the rhizoma bletillae polyploidy plants. According to the method, the rhizoma bletillae polyploidy plants can be obtained by taking the rhizoma bletillae seeds as material, the rhizoma bletillae polyploidy plants have remarkable polyploidy characteristics, and the preparation method is simple and has important guiding significance for the cultivation work of the new varieties of rhizoma bletillae; and the rhizoma bletillae polyploidy plants have higher application value.

Description

(1) Technical field [0001] The invention relates to a method for cultivating white and polyploid plants. (2) Background technology [0002] Baiji is the dry tuber of Bletilla sfriata (Thunb.) Reiehb.f., which has the effects of astringent, hemostasis, swelling and granulation. It is used to treat hemoptysis, hematemesis, traumatic bleeding, sore swelling, chapped skin embolism. Baiji not only has high medicinal value, but also has great ornamental value. It is an important raw material for my country's modern pharmaceutical industry and cosmetics industry. In recent years, with the development of traditional Chinese medicine in my country, the scope of clinical application of Baiji has been continuously expanded, and the demand for medicines of Baiji has increased sharply. However, the seeds of Baiji are very small and have no endosperm, so it is difficult to germinate and grow under natural conditions, and the cultivation of seedlings is relatively difficult. They only re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 蒋福升刘沁洪丁志山吕迪李伟平金波丁滨高承贤
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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