Inducing method for improving cell synchronization of tendril-leaved fritillary bulbs
A fritillaria and synchronization technology, applied to plant cells, etc., can solve the problem of low induction rate of polyploid homozygosity, and achieve the effect of increasing polyploid induction rate, high synchronization rate, and increasing division index
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Embodiment 1
[0019] Embodiment 1: a kind of induction method that improves the synchronization of fritillary mother cells, it comprises the following steps:
[0020] S1: Cut the callus induced by the scale leaves of Fritillaria sichuanensis into 0.5-2cm 2 The size of the callus is transferred to the culture medium that produces loose callus, the pH value is 5.6-6.5, and the culture conditions are: temperature 20-25°C, light for 8-12 hours per day, light intensity 1000-1800lx;
[0021] S2: The loose callus tissue obtained in step S1 was pressed by 5-20g·L -1 The amount of inoculation into MS+KT 0.5 ~ 1.0mg L -1 +2,4-D 0.5~2.0mg·L -1 + sucrose 10~25g·L -1 Suspension culture is carried out in liquid culture medium, pH value is 5.5-6.0, culture conditions: temperature 18-22 ℃, light 6-10h per day, light intensity 600-1000lx, shaker speed 80-100r / min , subculture once every 20-30 days;
[0022] S3: During the suspension culture process of step S2, choose to add 1-3mmol / L adenosine to the t...
Embodiment 2
[0024] Embodiment 2: a kind of induction method that improves the synchronization of fritillary mother cells, it comprises the following steps:
[0025] S1: Cut the callus induced by the scale leaves of Fritillaria sichuanensis into 0.5-2cm 2 Transfer to medium MS+6-BA 0.1~0.5mg·L for loose callus -1 +NAA 0.1~1.0mg·L -1 +2,4-D 1.0~4.0mg·L -1 + 30~50g·L sucrose -1 + Agar 4.5~6.0g·L -1 Cultivate in a medium with a pH value of 5.6-6.5, culture conditions: temperature 20-25°C, light for 8-12 hours per day, and light intensity of 1000-1800lx;
[0026] S2: The loose callus tissue obtained in step S1 was pressed by 5-20g·L -1 The amount of inoculation into MS+KT 0.5 ~ 1.0mg L -1 +2,4-D 0.5~2.0mg·L -1 + sucrose 10~25g·L -1 Suspension culture is carried out in liquid culture medium, pH value is 5.5-6.0, culture conditions: temperature 18-22 ℃, light 6-10h per day, light intensity 600-1000lx, shaker speed 80-100r / min , subculture once every 20-30 days;
[0027] S3: During the ...
Embodiment 3
[0029] Embodiment 3: a kind of induction method that improves the synchronization of fritillary mother cells, it comprises the following steps:
[0030] S1: Cut the callus induced by the scale leaves of Fritillaria sichuanensis into 0.5-2cm 2 Transfer to medium MS+6-BA 0.1mg·L for loose callus -1 +NAA 0.1mg·L -1 +2,4-D 1.0mg·L -1+ sucrose 30g·L -1 + Agar 4.5g·L -1 Cultivate in a medium with a pH value of 5.6-6.5, culture conditions: temperature 20°C, light for 8 hours a day, light intensity 1000lx;
[0031] S2: The loose callus obtained in step S1 was pressed by 5g·L -1 The amount of inoculation into MS+KT 0.5mg L -1 +2,4-D 0.5mg·L -1 + sucrose 10g·L -1 Suspension culture was carried out in liquid medium with a pH value of 5.5-6.0. Culture conditions: temperature at 18°C, light for 6 hours per day, light intensity of 600lx, and shaker speed of 80r / min, subculture once every 20 days ;
[0032] S3: During the suspension culture process of step S2, choose to add 1mmol / L...
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