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Sexual polyploidization breeding method for tetraploid petunias

A technology of petunia and tetraploid, which is applied in the field of sexual polyploidy breeding of tetraploid petunia, can solve the problems of asexual polyploidy, etc., and achieve wide genetic diversity, high ornamental quality and The effect of seed setting rate

Inactive Publication Date: 2014-10-01
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

However, there is no patent report on the sexual polyploidization of the annual herbaceous flower Petunia

Method used

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  • Sexual polyploidization breeding method for tetraploid petunias
  • Sexual polyploidization breeding method for tetraploid petunias
  • Sexual polyploidization breeding method for tetraploid petunias

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Embodiment 1

[0022] Embodiment 1: a kind of sexual polyploidy breeding method of tetraploid petunia, the method comprises the following steps:

[0023] (1) Determination of the flower bud size of diploid petunia during prophase I of meiosis: take the young flower buds of diploid petunia from 8:00 am to 10:00 am on a sunny day, measure their length and fix them with Carnoy’s fixative After 24 hours, the volume concentration is 90%, the volume concentration is 80% ethanol gradient dehydration, and finally transferred to the volume concentration of 70% ethanol and stored at 4°C for later use; the flower buds are bathed with 1 N hydrochloric acid at a constant temperature of 60°C for about 2-3 minutes, and rinsed with distilled water for 3-3 minutes. 4 times, the anthers were stripped from the flower buds, stained with 1% acetic acid magenta for routine staining, pressed, and flame-copied for 2 seconds for color separation, and the pollen mother cell meiosis stage was observed under a 100-fold ...

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Abstract

The invention discloses a sexual polyploidization breeding method for tetraploid petunias. The method comprises the following steps: firstly, determining the sizes and the morphological characteristics of flower buds at a reduction mitosis prophase I of diploid petunias to grasp the optimal treatment period; carrying out inducing treatment with colchicines with optimal concentration for proper times, observing the sizes and the morphological characteristics of petunia 2n pollen and n pollen of the petunias to rapidly distinguish and identify the 2n pollen; carrying out sexual polyploidization hybridizing; and identifying ploidies of hybridized F1 plant of the petunias by a simple and easy cytology identification method. With the adoption of the breeding method, the obtained sexual polyploidization hybridized F1 not only has the characteristics of large volume of a parent polyploidy organ, but also has the inheritable characters from a diploid male parent; compared with an autotetraploid obtained by an asexual polyploidization manner, the tetraploid petunias have wider genetic diversity, and higher ornamental value and seed setting rate.

Description

technical field [0001] The invention belongs to the field of horticultural plant breeding, and in particular relates to a sexual polyploid breeding method for tetraploid petunias. Background technique [0002] Plant polyploidy refers to the somatic cells containing three or more chromosome groups, which are widely used because of their hugeness, strong stress resistance, low fruitiness and high nutritional content. Polyploidy breeding is widely used in modern breeding It has broad prospects. At present, there are mainly two methods of artificially inducing plant polyploidy: asexual polyploidy and sexual polyploidy. [0003] (1) Somatic polyploidization (Somatic polyploidization), chromosome doubling occurs in the process of mitosis, that is, a polyploid plant is developed from a chromosome-doubled somatic cell. Common induction methods are physical and chemical. Physical methods include temperature shocks, mechanical trauma, ionizing radiation, non-ionizing radiation, cent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H1/08
Inventor 魏跃王全智颜志明杨宝林董慧嵇怡贾思振史红林
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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