Cultivation method for tetraploid Petunia hybrida
A breeding method and petunia technology, applied in the field of horticultural plant genetics and breeding, can solve the problems of great damage to the development of microspores, unsuitable use, and reduced seed setting rate, and achieve simple and easy operation, high ornamental value, and high yield. The effect of sex and seed set rate
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Embodiment 1
[0028] Embodiment 1: a kind of breeding method of tetraploid petunia
[0029] 1) Take the red Petunia hybrida 'U-IRIS Red' in the large-flowered diploid petunia iridescent series as the test object, and sow and plant according to conventional techniques;
[0030] 2) Enter the adult plant and cut off too many branches and weak branches on the plant, keep only 3 strong branches for each plant, remove the terminal buds of the branches, do not water or water less for 5 days before the mutagenesis, keep the soil dry and increase the soil moisture content If it is lower than 8%, immediately and fully water the soil after the mutagenesis treatment to keep the soil moist so that the soil moisture content reaches 20%, and the temperature is kept at about 25°C. The axillary buds on the plants are mutated with a chemical inducer, the chemical inducer is colchicine, and the final mass concentration is 0.1%. The method of mutagenesis is as follows: cover the leaf axils with absorbent cotton...
Embodiment 2
[0035] Embodiment 2: a kind of cultivation method of tetraploid petunia
[0036] 1) Take the blue Petunia hybrida 'U-IRIS Blue' of the large-flowered diploid petunia iridescent series as the test object, and sow and plant according to conventional techniques;
[0037] 2) Enter the adult plant and cut off too many branches and weak branches on the plant, keep only 4 strong branches for each plant, remove the terminal buds of the branches, do not water or water less for 6 days before the mutation, keep the soil dry and increase the soil moisture content less than 8%. , immediately after the mutagenesis treatment, fully water to keep the soil moist so that the soil moisture content reaches 22%, and the temperature is kept at about 30°C. The axillary buds on the plants are mutated with a chemical inducer, the chemical inducer is colchicine, and the final mass concentration is 0.2%. The method of mutagenesis is as follows: cover the leaf axils with absorbent cotton and closely co...
Embodiment 3
[0042] Embodiment 3: a kind of breeding method of tetraploid petunia
[0043] 1) Take the pink Petunia hybrida 'DOUBLE CASCADEPink' in the double-petunia double waterfall series as the test object, and sow and plant according to conventional techniques;
[0044] 2) Enter the adult plant and cut off too many branches and weak branches on the plant, keep only 5 strong branches for each plant, remove the terminal buds of the branches, do not water or water less for 7 days before the mutagenesis, keep the soil dry and increase the soil moisture content less than 8%. , immediately after the mutagenesis treatment, water the soil sufficiently to keep the soil moist so that the soil water content reaches 25%, and the temperature is kept at 32°C. The axillary buds on the plants are mutated with a chemical inducer, the chemical inducer is colchicine, and the final mass concentration is 0.3%. The method of mutagenesis is as follows: cover the leaf axils with absorbent cotton and make c...
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