The invention discloses application of a
protein or
gene for regulating and controlling
petunia discoloration in regulating and controlling
petunia discoloration and a cultivation method, and belongs to the technical field of
plant genetic engineering. According to the invention, a
gene PH7 for regulating the color change of
petunia is cloned from petunia, and belongs to ERD6L family
protein. A
CRISPR / Cas9
gene editing
system is utilized to obtain a PH7
gene knockout strain, the
petal color of the obtained PH7
gene knockout strain is not obviously different from that of a
wild type in the early flower development stage and the just blooming stage, but the petals of the knockout strain start to appear blue plaques 2-3 days after blooming, and gradually and completely become blue along with the progress of the flower development stage, and the petals of the knockout strain become blue. The PH7 gene plays an important role in regulating and controlling the formation of the petunia petunia color-changing flowers. The invention provides a new thought for the
molecular mechanism research of petunia hybridoma
flower formation regulation, and provides a new target for the cultivation of a new variety of petunia hybridoma flowers.