39 results about "Petunia hybrida" patented technology

The invention relates to the field of feeds for livestock and poultry and particularly relates to a formula feed for enhancing the disease resistance of a broiler breeder. The feed is prepared from the following raw materials in percentage by weight: 180-220 parts of hulled corns, 60-80 parts of cottonseed meal, 55-60 parts of soya-bean cakes, 65-70 parts of rice bran, 60-70 parts of wheat bran, 35-40 parts of corn stalks, 2-3 parts of dried leech powder, 3-4 parts of fish meal, 5-8 parts of carrot juice, 2-3 parts of pig liver powder, 1-2 parts of folium cortex eucommiae, 2-3 parts of glycyrrhiza uralensis, 1-2 parts of morus alba, 2-3 parts of Chinese chive seeds, 2-3 parts of chili powder, 2-3 parts of commelina communis, 3-4 parts of salsola collina, 3-4 parts of petunia hybrida vilm stems and leaves, 5-6 parts of single cream, 4-5 parts of dried red date powder, a proper amount of table salt and 4-5 parts of a phagostimulant. The formula feed provided by the invention is added with Chinese herbal medicine components capable of effectively enhancing the disease resistance and the antibacterial capability of the broiler breeder; any chemical medicine is not added; after a broiler breeder crowd is fed continuously, the morbidity is inhibited effectively.

The invention belongs to the field of the seed coating technology, specifically relates to the coating formulation of particulate flower seeds and the processing method. The invention is characterized in that, the product contains concave-convex soil 69-89wt%, calcium stearate 10-30wt% and bacillus 1%. The particulate flower seeds such as Petunia hybrida and Begonia semperflorens are coated in pills according to the invention, the treated coating seed is in bursting crack type, and not cracking in water, the invention does not impact seed germination, one seed rate, seeding containing rate and germination rate are high; the coating process does not take time, is particularly suitable for industrial seedling.

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.

The invention discloses separating and culturing methods for mesophyll protoplasts of petunia hybrida. The separating method for the protoplasts comprises following steps: tender leaves of the petunia hybrida are weighed, processed and subjected to enzymolysis with an enzymatic hydrolysate; a CPW (cell protoplast washing) medium is added to a solution obtained after enzymolysis, the solution is filtered and centrifuged, and a supernatant is absorbed; the CPW medium is added again for washing, centrifugation is performed, precipitates are collected, and the purified protoplasts are obtained. The culturing method for the protoplasts comprises steps as follows: the prepared protoplasts are added to a protoplast culture solution and are cultured for 30 d with a solid-liquid culture method in a dark environment at the temperature of 24 DEG C, and a miniature callus formed through culture is transferred to an enrichment medium; the miniature callus is cultured for 21 d and then is transferred to a differential medium; culturing is performed. With the adoption of the separating and culturing methods, the protoplasts with high activity and high yield can be obtained, the callus can be induced through culturing of the protoplasts, shoots can be differentiated finally, an experimental material is provided for study of further somatic cell fusion and the like of the petunia hybrida, and a foundation is laid for germplasm resource innovation of the petunia hybrida.

The invention discloses a herbaceous flower seed priming method. The method comprises the following steps: 1) carefully selecting seeds; 2) preparing a polyethylene glycol solution with an osmotic potential being between -0.5 and -2.0 Mpa; 3) pouring a PEG solution into a container, putting a semi-permeable membrane with a same size on the solution, putting the seeds to be treated on the semi-permeable membrane, allowing the container to stand at 10 DEG C-20 DEG C for 7-14 days; 4) drying at 25 DEG C for 24 hours, or directly sowing without drying. The invention adopts a semi-permeable membrane to control the water absorption process of the seeds, avoids direct contact of the seeds with the solution, is applicable to the priming treatment of high-value herbaceous flower seeds, and is especially applicable to seeds with mucus on surfaces such as pansy, and particle seeds such as petunia hybrida.

The invention provides a planting method for petunia hybrida. According to the invention, sowing, cutting or tissue culture propagation is adopted; sowing time is determined according to marketing time, e.g., if flowers are needed in May, greenhouse sowing is carried out in January, and if the flowers are needed in October, sowing is carried out in July; the number of seeds in each gram of seeds is 9000-10000, germination temperature is 20 to 22 DEG C, indoor potted sowing is adopted, and high-temperature sterilized mixed soil of culture soil, rotten leaf soil and fine sand is used; soil covering is not needed after sowing, glass or a transparent plastic plate is pressed on the soil, and germination starts in about 10 days; and when true leaves appear, room temperature is maintained in a range of 13 to 15 DEG C. The planting method for the petunia hybrida provided by the invention enables the petunia hybrida to have good quality.

The invention discloses a method for producing a petunia hybrida vilm potted flower. The method comprises the following steps of preparing a production facility and a culture substrate; seeding; performing seedling stage management; potting; performing potted flower management. A production flow for the petunia hybrida vilm potted flower is developed in the facility, i.e., a greenhouse, and culture management conditions in each flow step are controlled, so that the petunia hybrida vilm potted flower which is high in quality is obtained. The method is simple, feasible, and low in production cost; the production efficiency and the production capacity can be improved; a good economic benefit can be brought; the method is suitable for being popularized and used in production practice; the growth vigor of the produced potted flower is relatively strong; the period from seeding to a flowering period is short; the flowering period is long; the number of flowers is relatively large during the flowering period, and the effect of shielding leaves by the flowers is achieved.

The current invention is a novel approach termed “parallel screening” which allows simultaneously screening of a population for insertions in all genes cloned from that or a closely related organism. In order to test this approach, the flowering plant Petunia hybrida was used as a model system. Petunia hybrida line W137 contains a high copy number of the endogenous transposable element dTph1 and has been previously presented as a genetic tool. A 3D library of the plant genomic DNA of 1000 Petunia hybrida W137 plants was generated. The 3D library consists of 30 pools of DNA from 100 plants each. These were used to generate 30 pools of insertion flanking sequences by nested iPCR using a set of transposon-specific primers or by Transposon Display PCR. Insertions into a gene were detected by hybridizing the amplified insertion flanking sequences fixed to a filter with a gene-specific probe, an approach termed simple screening for insertion elements. Alternatively, the amplified insertion element flanking sequences were labeled and used as a probe to hybridize a filter displaying multiple gene targets, an approach termed parallel screening for insertion elements, which allows the simultaneous screening for insertions in all genes of an organism, appearing in a population of insertion mutants.

The present invention provides a cryopreservation method for in vitro shoot tips of petunia hybrida. The method, mainly based on droplet vitrification cryopreservation technology, includes the following steps of: expanding propagation of materials, stripping of shoot tips, performing pre-culture, loading, performing PVS2 processing and performing cryopreservation. Plant regeneration culture can be performed on the in vitro shoot tips of the petunia hybrida obtained by adopting the droplet vitrification cryopreservation technology in the invention, which indicates that the method in the invention is a method that can preserve resources of the species of the petunia hybrida with long-term security, stability, reliability, simplicity and effectiveness, regeneration seedlings being capable ofrecovering growth favorably, plant regeneration rate of the plant achieving more than 50%.

The invention discloses a method for culturing monopetalous petunia hybrida test-tube flowers. The method comprises the following steps: sterilizing seeds of a long-day monopetalous petunia hybrida variety serving as an explant; putting the seeds on the surface of an MS medium and germinating; cutting stems with 2-3 leaves after sprouts grow out, inserting the stems into a subculture medium for continuous subculture; cutting stem tips with 4-6 leaves from subculture seedlings, and inoculating to a flower bud medium; and generating flower buds and blooming by cultural environmental induction to finally form test-tube flowers. The method has the beneficial effects that: the plant size, flower bud induction and flower bud blooming of petunia hybrida in test tubes are manually controlled by virtue of a tissue culture technology so as to finally obtain test-tube flowers; the test-tube flowers have the characteristics that flowers are of various colors, the flowering phase can last for one month, and blooming is continuous; and the method is simple and reliable, has production practicality, and can increase new varieties of test-tube flowers.

The invention relates to application for repairing lead-polluted soil by chemical-enhanced flower plants, in particular to application for repairing heavy metal lead-polluted soil by using dibenzyloxyphosphoiyl butane tricarboxylic acid-enhanced and enriched plant petunia hybrida. The petunia hybrida is planted on the soil containing pollutant lead; when the plant grows to the mature period, dibenzyloxyphosphoiyl butane tricarboxylic acid is applied to the soil for activating the lead in the soil; most lead in the polluted soil is absorbed through the root system of the petunia hybrida, and is transferred to an organ above the ground; and the organ of the plant above the ground is shifted away from the polluted soil to realize the purpose of removing the pollutant lead from the soil. The dibenzyloxyphosphoiyl butane tricarboxylic acid-enhanced and enriched plant petunia hybrida is used for treating the lead-polluted soil; and such advantages as strong operability and prevention of wind erosion and water erosion of the polluted soil are achieved.

The invention discloses a method of propagating Petunia hybrida by layering; the method comprises: vertically cutting a hard portion of a base of a branch of Petunia hybrida to obtain a wound, with epidermis cut through to expose xylem, applying a rooting agent to the wound, covering the wound with substrate until the covered portion roots, separating the lower end of the rooting portion of the branch from a parent body after rooting, and performing transplanting. The method has the advantages of high seedling growth speed, early flowering, high survival rate, good convenience of operation, and low cost.

The invention relates to boric fertilizer. The boric fertilizer is characterized by being prepared from soybeans, orange peel, pine saw dust, an enzyme decomposition maturing agent and a borax solution through fermentation. The boric fertilizer and organic fertilizer take effect synergistically, tea quality can be improved, the yield of blueberries and the content of anthocyanin can be increased, and florescence of petunia hybrida can be prolonged.

The application belongs to the technical field of horticulture, and particularly relates to a standardized tissue culture method for improving genetic transformation efficiency of petunia hybrida. The tissue culture method is suitable for tissue culture application of petunia during transgenic genetic transformation, and specifically comprises the steps of explant selection, dip dyeing liquid preparation, leaf dip dyeing and co-culture, screening culture, rooting culture, acclimatization and transplantation and the like. In the application, by taking specific plasmid construction as an example, key factors of related tissue culture technologies influencing genetic transformation efficiency in a petunia genetic transformation process are discussed and optimized, so that a certain technical foundation can be laid for standardized operation and standardized tissue culture system construction in the related genetic transformation process; and a certain technical foundation can be laid for petunia hybridoma related genetic transformation system construction and new petunia hybridoma variety breeding, so that the method has relatively good scientific research value and practical significance.

The invention aims to provide a molecular method for regulating and controlling the flowering rhythm of petunia hybrida. In the implementation method of the molecular method, the molecular method forregulating and controlling the flowering rhythm of plants by using flower specific expression promoters and circadian clock genes together is realized in combination with the means of genetic transformation. The molecular method comprises the steps of promoter synthesis, LHY gene amplification and carrier construction, genetic transformation is carried out by using the important flower petunia hybrida with simple tissue and cell operation technology, short life cycle and clear genetic background as a transgenic model plant, and finally a transgenic plant of which the flowering time changes isobtained. The expression of the circadian clock gene LHY capable of regulating and controlling the flowering rhythm in petunia hybrida is promoted by the flower specific expression promoters, and theflowering time of the obtained transgenic plant is a new product with obvious difference compared with that of a contrast plant. Through the statistics of the flowering time of the obtained positive transgenic plant, the lowering time of the transgenic plant compared with that of a check plant is delayed by nearly four hours.

The invention relates to the field of feeds for livestock and poultry, in particular to a formula feed for strengthening disease resistance of breeding broilers. The feed is prepared from the following raw materials of 180-220 parts of peeled corn, 60-80 parts of cottonseed meal, 55-60 parts of bean cakes, 65-70 parts of rice bran, 60-70 parts of wheat bran, 35-40 parts of corn stalks, 2-3 parts of dried leech powder, 3-4 parts of fish meal, 5-8 parts of carrot juice, 2-3 parts of pork liver powder, 1-2 parts of folium eucommiae, 2-3 parts of licorice roots, 0.001-0.005 part of glyceryltri(2-ethylhexanoate), 0.0.001-0.003 part of ferric ammonium citrate, 1-2 parts of white mulberry root bark, 2-3 parts of leek seeds, 2-3 parts of papaya powder, 2-3 parts of dayflower herbs, 3-4 parts of salsola collina, 3-4 parts of petunia hybrida stalks and leaves, 5-6 parts of dried pumpkins, 4-5 parts of dried red jujube powder, an appropriate amount of table salt and 4-5 parts of a phagostimulant. According to the formula feed provided by the invention, Chinese herbal medicine ingredients which can effectively strengthen the disease resistance and antibacterial capacity of the breeding broilers are added, and no chemical medicines are added, so that after the formula feed is continuously fed to broiler crowds, the attack rate is effectively restrained.

The invention relates to establishment of an efficient genetic transformation system of petunia hybrida 'Mellin'. The method comprises the following steps: (1) dark culture of leaves; (2) preparation of an infection solution; (3) infection; (4) co-culture; (5) screening and culturing; (6) resistant plant acquisition; and (7) positive plant acquisition. According to the method, the genetic transformation rate of petunia hybrida 'Mellin' is improved through dark culture of leaves, bacterial liquid infection time, kanamycin sensitivity detection, antibiotic concentration screening and the like, the problems of low transformation rate and differentiation rate, long breeding time and the like are solved, the rooting rate of resistant plants reaches 66.24% by the system, and positive transgenic plants can be obtained within 2.5 months.

The invention discloses a method for producing a petunia hybrida vilm potted flower. The method comprises the following steps of preparing a production facility and a culture substrate; seeding; performing seedling stage management; potting; performing potted flower management. A production flow for the petunia hybrida vilm potted flower is developed in the facility, i.e., a greenhouse, and culture management conditions in each flow step are controlled, so that the petunia hybrida vilm potted flower which is high in quality is obtained. The method is simple, feasible, and low in production cost; the production efficiency and the production capacity can be improved; a good economic benefit can be brought; the method is suitable for being popularized and used in production practice; the growth vigor of the produced potted flower is relatively strong; the period from seeding to a flowering period is short; the flowering period is long; the number of flowers is relatively large during the flowering period, and the effect of shielding leaves by the flowers is achieved.

The invention relates to the technical field of biological prevention and control, and particularly discloses application of petunia hybrida to trapping and killing thrips. According to the application, the petunia hybrida and target plants to be protected are planted in a matched manner, the petunia hybrida is used for trapping and killing the thrips, and therefore the thrips are prevented and treated in a biological manner. A biological prevention and control method for the thrips specifically comprises the following steps of cultivating petunia hybrida plants for trapping and killing the thrips; planting the target plants to be protected; and planting the petunia hybrida plants and the target plants to be protected in the matched manner. According to the application, under the conditionthat pesticide treatment is not needed, the thrips can be remarkably controlled only through a biological control means, and good social benefits and ecological benefits are achieved. The method hasthe advantages of environmental friendliness, economical performance, high efficiency, simplicity and convenience in operation, easiness in popularization and the like.

The invention discloses a petunia zinc finger protein gene PhZFP1 and its application in improving plant cold resistance. Gene cloned from cold-resistant petunia "H" PhZFP1 , whose nucleotide sequence is shown in SEQ ID.1, using the Agrobacterium-mediated genetic transformation method to transform petunia, the transgenic plants obtained, by measuring the electrical conductivity, low temperature treatment survival rate, etc., and NBT staining, it is found that PhZFP1 The cold resistance of super-table transgenic lines has been significantly improved, breaking through the barriers of traditional breeding methods, and providing important genetic resources for plant cold resistance genetic engineering.

The invention discloses a petunia rejuvenation agent and a use method thereof. The petunia rejuvenation agent is composed of gibberellin, triacontanol and brassinolide, and the weight ratio of the three components is: 10.0~20.0:1.0~2.0:0.5~1.5, the preferred ratio is 15.0:1.5:1.0, the three components are calculated according to the weight ratio of 100% purity. When used, it is diluted at a concentration of 15-25 µg·L-1, and then sprayed on cut and trimmed petunias. The operation should be carried out in the evening on cloudy or sunny days. After spraying, it is guaranteed that there will be no rain within 24 hours, and artificial sprinkler irrigation will not be carried out during this period. After 24 hours, apply water and fertilizer once; after 10 days, apply water and fertilizer once, and then spray petunia regeneration agent again after 0.5-1.0 hours, and the spraying concentration is the same as the first time. The rest of the time is for routine fertilizer and water management and pest control. The rejuvenating agent has stable dosage form, simple and convenient operation, low use cost and remarkable regeneration effect, and the single agents in the formulation have a synergistic effect, and is a good petunia regenerating agent.

The invention discloses a method for culturing monopetalous petunia hybrida test-tube flowers. The method comprises the following steps: sterilizing seeds of a long-day monopetalous petunia hybrida variety serving as an explant; putting the seeds on the surface of an MS medium and germinating; cutting stems with 2-3 leaves after sprouts grow out, inserting the stems into a subculture medium for continuous subculture; cutting stem tips with 4-6 leaves from subculture seedlings, and inoculating to a flower bud medium; and generating flower buds and blooming by cultural environmental induction to finally form test-tube flowers. The method has the beneficial effects that: the plant size, flower bud induction and flower bud blooming of petunia hybrida in test tubes are manually controlled by virtue of a tissue culture technology so as to finally obtain test-tube flowers; the test-tube flowers have the characteristics that flowers are of various colors, the flowering phase can last for one month, and blooming is continuous; and the method is simple and reliable, has production practicality, and can increase new varieties of test-tube flowers.

The invention discloses a feed special for leiothrix lutea and a preparation method thereof. The feed comprises the following formula components in parts by mass: 48-50 parts of petunia hybrida, 21-23 parts of sesame straw, 4-6 parts of szechwan pepper, 25-27 parts of anthurium scherzerianum, 27-29 parts of white flower patrinia herb, 20-22 parts of white mustard seeds, 15-17 parts of apples, 8-10 parts of onion seed powder, 29-31 parts of bovine bone meal, 16-18 parts of protein mud and 220-240 parts of water. The feed disclosed by the invention is rich in nutrient substance and scientific in proportion, and a formula contains szechwan pepper components, so that the effects of warming the spleen and stomach for dispelling cold, and relieving itching and removing fishy smell can be achieved, and the palatability of the feed can be effectively improved.

The invention discloses a zinc finger protein gene PhZFP1 of petunia hybrida and application of the zinc finger protein gene PhZFP1 in improving cold resistance performance of plants. The gene PhZFP1is coned from cold-resistant petunia hybrida 'H', the nucleotide sequence of the gene PhZFP1 is shown in SEQ ID. 1, and an agrobacterium-mediated genetic transformation method is utilized for transforming the petunia hybrida to obtain a transgenic plant. Through measurement of conductivity, low-temperature treatment survival rate and the like and NBT staining, it is discovered that the cold resistance performance of a PhZFP1 overexpression transgenic plant line is significantly improved, obstacles of a traditional seed culturing means are broken through, and an important gene resource is provided for cold resistance gene engineering of plants.

The invention discloses an application of PhCLV3 and PhCLV1 genes in regulating and controlling the ornamental value of petunia plants. The invention finds that the PhCLV3 and PhCLV1 genes are knocked out by using a gene editing technology, a single mutant with lost functions shows that the number of petals is increased, the phenotype of double mutants with lost functions of the two genes is more violent, scarce head-shaped flower buds are presented, the petals are double petals, the petal area is increased, the flowering density is increased or the plant height is reduced, and the number of the petals is increased. The ornamental value of petunia as a flower plant is greatly improved, and it is indicated that the PhCLV3 and PhCLV1 genes have important regulation and control effects in the growth and development process of petunia. Therefore, the method has a good application prospect in the aspect of character improvement of petunia hybrida flowers.

The invention belongs to the technical field of urban domestic sludge resource utilization and garden production, and particularly relates to a petunia hybrida culture medium and a preparation method and use thereof. The petunia hybrida culture medium comprises organic nutrient soil, coco coir, perlite and garden soil. Relative to the petunia hybrida culture medium, the content of the organic nutrient soil is 50vt%-70vt% and the content of the coco coir is 10vt%-30vt%; and a volume ratio of the coco coir and the perlite and the garden soil is (1-3):(0.8-1.2):(0.8-1.2). The culture medium is used for cultivating petunia hybrida. Plants have good growth vigor and an advanced and prolonged flowering phase. The method is simple and easy to implement. The petunia hybrida culture medium has a low cost and an excellent effect, and provides application of organic nutrient soil (sludge products) in garden flowers. A theoretical basis is provided for popularization and application of the culture medium.

The invention discloses a cultivation method for tetraploid Petunia hybrida. The cultivation method comprises the following steps: mutagenizing the axillary buds of a diploid Petunia hybrida plant with a chemical inducer, and after mutagenized axillary buds germinate and grow into branches, identifying and screening out tetraploid branches; with the tetraploid Petunia hybrida with good fertility as a female parent, emasculating the tetraploid Petunia hybrida, smearing mature 2n pollen from mutagenized tetraploid branches on the stigma of the female parent, wherein F1 hybrid seeds are obtainedafter a plant bears fruit; and screening a tetraploid plant from the generation F1, and carrying out pedigree selection so as to obtain a tetraploid Petunia hybrida variety with excellent characters.The method provided by the invention can improve the mutagenesis rate of a polyploid; and a tetraploid variety cultivated by using the method provided by the invention has more extensive genetic diversities, inherits a plurality of excellent characters of a diploid male parent, and has high ornamental properties and high seed setting rate; meanwhile, the cultivation method provided by the invention has low requirements on an operation site, instruments and equipment, and is relatively simple and practicable in the process of operation.

The invention relates to an organic fermented boron fertilizer and a method for preparing the organic boron fertilizer for blueberries. The boron fertilizer is characterized by being prepared by soybean, orange peels, pine sawdust, an enzyme decomposition agent and a borax solution through fermentation. The boron fertilizer and an organic fertilizer produce a synergic effect, so that the tea quality can be improved, the blueberry yield and anthocyanin content can be increased, and a flowering phase of petunia hybrida can be prolonged.

The invention relates to the technical field of petunia hybrida cultivation, in particular to a cultivation method of tetraploid petunia hybrida which comprises the steps that tetraploid petunia hybrida seeds are soaked in a hydrogen peroxide aqueous solution, after cleaning, the tetraploid petunia hybrida seeds are soaked in clear water for accelerating germination at variable temperature and variable light, and tetraploid petunia hybrida seeds subjected to pre-germination acceleration are obtained; the culture soil is flatly prepared into a seedbed with the height of 25-35 cm, tetraploid petunia hybrida seeds subjected to pre-germination acceleration are sown, and water is sprayed till the soil is thoroughly drenched; after the tetraploid petunia hybrida seeds germinate, when petunia hybrida seedlings have 4-5 true leaves, the petunia hybrida seedlings can be separately planted, and finished plant cultivation can be carried out. According to the preparation method, the tetraploid petunia hybrida can be promoted to germinate through hydrogen peroxide pretreatment and variable-temperature and variable-light pre-germination, and the method has the advantages of high germination rate and high yield.

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.