10 results about "Petunia hybrida" patented technology

Disclosed in the present invention are the use of an OBF1 transcription factor in plant chromosome doubling and a transgenic plant cultivation method. The present invention introduces the OBF1 gene into plant cells or tissue under treatment with kanamycin, so as to obtain a transgenic cell line or transgenic plant with doubled chromosomes, the gene sequence of the OBF1 transcription factor being shown as SEQ ID NO: 1, and the plant being Petunia hybrida, Solanum lycopersicum and Nicotiana tabacum.

The invention discloses a petunia hybrida PhERF110 gene and application of the petunia hybrida PhERF110 gene in regulation and control of plant types, and belongs to the technical field of gene engineering. The invention provides a petunia PhERF110 gene, the nucleotide sequence of the petunia PhERF110 gene is shown as SEQ ID NO.1, and the full length of the petunia PhERF110 gene is 1386bp. An overexpression vector and an RNAi vector of the Petunia hybrida PhERF110 gene are respectively constructed, a plant transformation experiment is carried out, and experiment results show that after the PhERF110 gene is overexpressed, the number of branches of a transgenic plant is remarkably increased, but PhERF110 RNAi causes the number of branches of the transgenic plant to be remarkably reduced, that is, the PhERF110 gene provided by the invention is capable of positively regulating and controlling the branch development of the plant. The invention provides a new gene resource for cultivating transgenic plants with increased branches, and has great significance in plant type regulation and control.

The invention provides a promoter pchsA derived from petunia, a gene expression cassette of betacyanin and application of the gene expression cassette, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the promoter pchsA is shown as SEQ ID No.1; according to the invention, a petunia organ specific promoter pchsA is utilized to construct a gene expression cassette of the betacyanin, so that heterologous synthesis of the betacyanin is successfully driven in tobacco, and expression and accumulation of the betacyanin in tissues of roots, stems, leaves and flowers of the tobacco are realized. According to the invention, a new transgenic tobacco germplasm capable of efficiently accumulating the betacyanin is created, a new material is provided for plant bioreactor production of the high-value pigment (betacyanin), and meanwhile, a method for rapidly, intuitively and visually detecting the activity of the promoter is established.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for improving high-temperature flowering quality of petunia hybrida, the regulator comprises 0.1 part by weight of a component A, 33 parts by weight of a component B and more than or equal to 100 parts by weight of a component C; the component A is selected from at least one of brassinolide and an isomer thereof, the component B is benzylaminopurine, and the component C is an amino acid composition. According to the invention, brassinolide, benzylaminopurine and amino acid are compounded for use, and through mutual cooperation of different action ways and mechanisms, the flowering attenuation rate of petunia hybrida in a high-temperature environment is reduced, the flowering amount is obviously increased, and the yield of petunia hybrida is increased. Meanwhile, the blooming ability is also improved in a continuous high-temperature environment.

The present invention relates to Calibrachoa plants displaying at least one inflorescence with a radially symmetric pattern along the center of the fused petal margins, wherein the pattern extends from the center of the inflorescence and does not fade during the life of the inflorescence.

This invention relates to the field of plant genetic engineering technology, specifically to a knockout method. PhAGL17 Application of genes in improving petunia traits. This invention is the first to discover that knocking out wild-type petunias... PhAGL17 Genes can significantly reduce plant height and increase calyx size, which helps control the overall compact shape of the plant and beautify the landscape.

The invention discloses application of a protein or gene for regulating and controlling petunia discoloration in regulating and controlling petunia discoloration and a cultivation method, and belongs to the technical field of plant genetic engineering. According to the invention, a gene PH7 for regulating the color change of petunia is cloned from petunia, and belongs to ERD6L family protein. A CRISPR / Cas9 gene editing system is utilized to obtain a PH7 gene knockout strain, the petal color of the obtained PH7 gene knockout strain is not obviously different from that of a wild type in the early flower development stage and the just blooming stage, but the petals of the knockout strain start to appear blue plaques 2-3 days after blooming, and gradually and completely become blue along with the progress of the flower development stage, and the petals of the knockout strain become blue. The PH7 gene plays an important role in regulating and controlling the formation of the petunia petunia color-changing flowers. The invention provides a new thought for the molecular mechanism research of petunia hybridoma flower formation regulation, and provides a new target for the cultivation of a new variety of petunia hybridoma flowers.

The invention discloses application of a PhZPT2-5 gene in regulation and control of anthocyanin synthesis of petunia hybrida, and belongs to the technical field of plant gene genetic engineering. The invention finds that the PhZPT2-5 gene is remarkably related to the anthocyanin synthesis of petunia, and experiments find that the anthocyanin synthesis can be reduced by silencing the PhZPT2-5 gene, the anthocyanin synthesis can be promoted by overexpressing the PhZPT2-5 gene, petunia with different colors can be obtained by regulating the expression of the PhZPT2-5 gene in the petunia, and a foundation is laid for breeding new varieties of petunia.

The invention provides an engineering bacterium for synthesizing phloretin as well as a construction method and application of the engineering bacterium. The engineering bacteria comprise escherichia coli and genes which are co-expressed in the escherichia coli and are used for coding tyrosine deaminase, p-coumaroyl-coenzyme A ligase, chalcone synthase and reductase, the tyrosine deaminase is derived from rhodotorula or hypericum monogynum, the p-coumaroyl-coenzyme A ligase is derived from arabidopsis A.thaliana, petunia or apple, and the chalcone synthase is derived from chalcone synthase. The chalcone synthase gene is derived from petunia hybrida, and the reductase gene is derived from clostridium or acetyl clostridium. Efficient synthesis of phloretin can be realized by utilizing the engineering bacteria, and the yield of the phloretin synthesized from the beginning can reach 244.55 mg / L. The invention also provides an application of the engineering bacterium in biosynthesis of phloretin derivatives such as 3-hydroxy phloretin, hesperetin dihydrochalcone, hesperetin dihydrochalcone glucoside, neohesperidin dihydrochalcone and the like.