39 results about "Petunia hybrida" patented technology

The invention discloses a method for producing a petunia hybrida vilm potted flower. The method comprises the following steps of preparing a production facility and a culture substrate; seeding; performing seedling stage management; potting; performing potted flower management. A production flow for the petunia hybrida vilm potted flower is developed in the facility, i.e., a greenhouse, and culture management conditions in each flow step are controlled, so that the petunia hybrida vilm potted flower which is high in quality is obtained. The method is simple, feasible, and low in production cost; the production efficiency and the production capacity can be improved; a good economic benefit can be brought; the method is suitable for being popularized and used in production practice; the growth vigor of the produced potted flower is relatively strong; the period from seeding to a flowering period is short; the flowering period is long; the number of flowers is relatively large during the flowering period, and the effect of shielding leaves by the flowers is achieved.

The invention discloses a petunia zinc finger protein gene PhZFP1 and its application in improving plant cold resistance. Gene cloned from cold-resistant petunia "H" PhZFP1 , whose nucleotide sequence is shown in SEQ ID.1, using the Agrobacterium-mediated genetic transformation method to transform petunia, the transgenic plants obtained, by measuring the electrical conductivity, low temperature treatment survival rate, etc., and NBT staining, it is found that PhZFP1 The cold resistance of super-table transgenic lines has been significantly improved, breaking through the barriers of traditional breeding methods, and providing important genetic resources for plant cold resistance genetic engineering.

The invention discloses a petunia rejuvenation agent and a use method thereof. The petunia rejuvenation agent is composed of gibberellin, triacontanol and brassinolide, and the weight ratio of the three components is: 10.0~20.0:1.0~2.0:0.5~1.5, the preferred ratio is 15.0:1.5:1.0, the three components are calculated according to the weight ratio of 100% purity. When used, it is diluted at a concentration of 15-25 µg·L-1, and then sprayed on cut and trimmed petunias. The operation should be carried out in the evening on cloudy or sunny days. After spraying, it is guaranteed that there will be no rain within 24 hours, and artificial sprinkler irrigation will not be carried out during this period. After 24 hours, apply water and fertilizer once; after 10 days, apply water and fertilizer once, and then spray petunia regeneration agent again after 0.5-1.0 hours, and the spraying concentration is the same as the first time. The rest of the time is for routine fertilizer and water management and pest control. The rejuvenating agent has stable dosage form, simple and convenient operation, low use cost and remarkable regeneration effect, and the single agents in the formulation have a synergistic effect, and is a good petunia regenerating agent.

The invention discloses a method for culturing monopetalous petunia hybrida test-tube flowers. The method comprises the following steps: sterilizing seeds of a long-day monopetalous petunia hybrida variety serving as an explant; putting the seeds on the surface of an MS medium and germinating; cutting stems with 2-3 leaves after sprouts grow out, inserting the stems into a subculture medium for continuous subculture; cutting stem tips with 4-6 leaves from subculture seedlings, and inoculating to a flower bud medium; and generating flower buds and blooming by cultural environmental induction to finally form test-tube flowers. The method has the beneficial effects that: the plant size, flower bud induction and flower bud blooming of petunia hybrida in test tubes are manually controlled by virtue of a tissue culture technology so as to finally obtain test-tube flowers; the test-tube flowers have the characteristics that flowers are of various colors, the flowering phase can last for one month, and blooming is continuous; and the method is simple and reliable, has production practicality, and can increase new varieties of test-tube flowers.

The invention discloses a cultivation method for tetraploid Petunia hybrida. The cultivation method comprises the following steps: mutagenizing the axillary buds of a diploid Petunia hybrida plant with a chemical inducer, and after mutagenized axillary buds germinate and grow into branches, identifying and screening out tetraploid branches; with the tetraploid Petunia hybrida with good fertility as a female parent, emasculating the tetraploid Petunia hybrida, smearing mature 2n pollen from mutagenized tetraploid branches on the stigma of the female parent, wherein F1 hybrid seeds are obtainedafter a plant bears fruit; and screening a tetraploid plant from the generation F1, and carrying out pedigree selection so as to obtain a tetraploid Petunia hybrida variety with excellent characters.The method provided by the invention can improve the mutagenesis rate of a polyploid; and a tetraploid variety cultivated by using the method provided by the invention has more extensive genetic diversities, inherits a plurality of excellent characters of a diploid male parent, and has high ornamental properties and high seed setting rate; meanwhile, the cultivation method provided by the invention has low requirements on an operation site, instruments and equipment, and is relatively simple and practicable in the process of operation.