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Method for obtaining regeneration plants of petunia hybrida by anther culture

A technology for regenerating petunia plants, applied in the agricultural field, can solve the problems of low regeneration rate and no regenerated petunia plants, and achieve the effect of improving breeding efficiency and shortening the period of hybrid breeding

Inactive Publication Date: 2013-12-04
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on petunia anther culture has a certain research foundation abroad, but there are only 3 related papers reported in China, and the regeneration rate is quite low
In addition, the author did not obtain petunia regenerated plants cultured by anthers according to the method described.

Method used

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  • Method for obtaining regeneration plants of petunia hybrida by anther culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, a kind of method that obtains petunia regenerated plant by anther culture, carries out following steps successively:

[0031] A) low temperature pretreatment and disinfection, the following steps are carried out successively:

[0032] 1) The selected petunia material is the red color of the Meilin series, sow seedlings in December, and start in late April of the following year. In the morning on a sunny day, select flower buds with a longitudinal diameter of 0.8cm-1.3cm (the flower buds in this period Most of them are in the single-core marginal stage),

[0033] 2), put the above-mentioned flower buds into plastic bags, and put them into a refrigerator at 5°C for low-temperature pretreatment for 2 days;

[0034]3), the flower buds after the above-mentioned low-temperature pretreatment are first placed under tap water and rinsed for 5 minutes, then rinsed with distilled water for 3 times, and then blotted with absorbent paper; Sterilize for 1 min; then ri...

Embodiment 2

[0050] Embodiment 2, a kind of method that obtains petunia regenerated plant by anther culture, carries out following steps successively:

[0051] A), low temperature pretreatment and disinfection, carry out the following steps successively:

[0052] 1), the selected petunia material is Meilin series blue, sow seedlings in December, and start in late April of the following year. In the morning on a sunny day, select flower buds with a longitudinal diameter of 0.8cm-1.3cm (the The flower buds are mostly in the uninucleate stage),

[0053] 2), put the above-mentioned flower buds into a plastic bag, and put them into a refrigerator at 5°C for low-temperature pretreatment for 3 days;

[0054] 3), the flower buds after the above-mentioned low-temperature pretreatment are first placed under tap water and rinsed for 5 minutes, then rinsed with distilled water for 3 times, and then blotted with absorbent paper; Sterilize for 1 min; then rinse with sterile distilled water for 3 times...

Embodiment 3

[0068] Example 3. Change the differentiation medium in Step C of Example 1 to: Nitsch basic medium + 7g / L agar + 20g / L maltose + 0.1mg / L IBA + 1.0mg / L 6-BA + hydrolyzed casein 0.5 g / L, the pH value is 5.8. Change 1.0mg / LTDZ to 1.0mg / L 6-BA. All the other are with embodiment 1.

[0069] The result is:

[0070] In this case, the callus induction rate can reach 40%, and the callus differentiation rate can reach 33%. The survival rate can reach more than 70%.

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Abstract

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to a method for obtaining regenerated plants of petunias through anther culture. Background technique [0002] Petunia (Petunia hybrida Vilm), also known as Bike eggplant and Ganoderma lucidum peony, is a perennial herb belonging to the genus Petunia in the family Solanaceae. Extensive management is an excellent and important flower bed greening grass flower in my country at present, and it can also be used as potted flowers. Petunia is more heat-resistant and is a commonly used greening flower in summer and autumn in my country. Because petunia has obvious heterosis and strong ornamental value, most petunia seeds currently on the market are F1 generation, and they are mainly imported from abroad. The key to hybrid breeding using hybrid vigor is to obtain pure parental parents (inbred lines) with excellent traits. In order to obtain the inbred line, according to the conventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘辉沈国正傅巧娟陈一沈莲佳
Owner HANGZHOU ACAD OF AGRI SCI
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