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A kind of breeding method of tetraploid petunia

A breeding method and petunia technology, applied in the field of horticultural plant genetics and breeding, can solve the problems of great damage to the development of microspores, unsuitable use, and reduced seed setting rate, and achieve simple and easy operation, high ornamental value, and high yield. The effect of sex and seed set rate

Active Publication Date: 2021-03-19
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] At present, excellent diploid petunia varieties such as the double-petaled "Double Waterfall" series and the large-flowered "Dream" series have high ornamental value but less pollen, less seed set, and low tolerance. The asexual polyploidization method is adopted. After the seed setting rate is further reduced and there are almost no offspring, it is not suitable for use; if the sexual polyploidization method is used, the young buds are treated with mutagen to cause greater damage to the development of microspores, and the pollen is further reduced. After flowering, there is no pollen, and it is not suitable for use.

Method used

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  • A kind of breeding method of tetraploid petunia

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Effect test

Embodiment 1

[0028] Embodiment 1: a kind of breeding method of tetraploid petunia

[0029] 1) Take the red Petunia hybrida 'U-IRIS Red' in the large-flowered diploid petunia iridescent series as the test object, and sow and plant according to conventional techniques;

[0030] 2) Enter the adult plant and cut off too many branches and weak branches on the plant, keep only 3 strong branches for each plant, remove the terminal buds of the branches, do not water or water less for 5 days before the mutagenesis, keep the soil dry and increase the soil moisture content If it is lower than 8%, immediately and fully water the soil after the mutagenesis treatment to keep the soil moist so that the soil moisture content reaches 20%, and the temperature is kept at about 25°C. The axillary buds on the plants are mutated with a chemical inducer, the chemical inducer is colchicine, and the final mass concentration is 0.1%. The method of mutagenesis is as follows: cover the leaf axils with absorbent cotton...

Embodiment 2

[0035] Embodiment 2: a kind of cultivation method of tetraploid petunia

[0036] 1) Take the blue Petunia hybrida 'U-IRIS Blue' of the large-flowered diploid petunia iridescent series as the test object, and sow and plant according to conventional techniques;

[0037] 2) Enter the adult plant and cut off too many branches and weak branches on the plant, keep only 4 strong branches for each plant, remove the terminal buds of the branches, do not water or water less for 6 days before the mutation, keep the soil dry and increase the soil moisture content less than 8%. , immediately after the mutagenesis treatment, fully water to keep the soil moist so that the soil moisture content reaches 22%, and the temperature is kept at about 30°C. The axillary buds on the plants are mutated with a chemical inducer, the chemical inducer is colchicine, and the final mass concentration is 0.2%. The method of mutagenesis is as follows: cover the leaf axils with absorbent cotton and closely co...

Embodiment 3

[0042] Embodiment 3: a kind of breeding method of tetraploid petunia

[0043] 1) Take the pink Petunia hybrida 'DOUBLE CASCADEPink' in the double-petunia double waterfall series as the test object, and sow and plant according to conventional techniques;

[0044] 2) Enter the adult plant and cut off too many branches and weak branches on the plant, keep only 5 strong branches for each plant, remove the terminal buds of the branches, do not water or water less for 7 days before the mutagenesis, keep the soil dry and increase the soil moisture content less than 8%. , immediately after the mutagenesis treatment, water the soil sufficiently to keep the soil moist so that the soil water content reaches 25%, and the temperature is kept at 32°C. The axillary buds on the plants are mutated with a chemical inducer, the chemical inducer is colchicine, and the final mass concentration is 0.3%. The method of mutagenesis is as follows: cover the leaf axils with absorbent cotton and make c...

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Abstract

The invention discloses a cultivation method for tetraploid Petunia hybrida. The cultivation method comprises the following steps: mutagenizing the axillary buds of a diploid Petunia hybrida plant with a chemical inducer, and after mutagenized axillary buds germinate and grow into branches, identifying and screening out tetraploid branches; with the tetraploid Petunia hybrida with good fertility as a female parent, emasculating the tetraploid Petunia hybrida, smearing mature 2n pollen from mutagenized tetraploid branches on the stigma of the female parent, wherein F1 hybrid seeds are obtainedafter a plant bears fruit; and screening a tetraploid plant from the generation F1, and carrying out pedigree selection so as to obtain a tetraploid Petunia hybrida variety with excellent characters.The method provided by the invention can improve the mutagenesis rate of a polyploid; and a tetraploid variety cultivated by using the method provided by the invention has more extensive genetic diversities, inherits a plurality of excellent characters of a diploid male parent, and has high ornamental properties and high seed setting rate; meanwhile, the cultivation method provided by the invention has low requirements on an operation site, instruments and equipment, and is relatively simple and practicable in the process of operation.

Description

technical field [0001] The invention relates to a method for cultivating tetraploid petunias, belonging to the field of horticultural plant genetics and breeding. Background technique [0002] Petunia (Petunia hybrida Vilm.) is a plant of the genus Petunia in the family Solanaceae, and is an annual herbaceous flower. Petunia has huge flowers, rich colors, and many flower patterns. At present, petunia has become the main potted flower and decorative plant in the world. It is widely used in the beautification and greening of courtyards, open-air flower beds, and squares. The king of flower beds in the world". my country's petunia began to be introduced and cultivated in small quantities in the early 20th century. In the 1980s, new varieties were gradually introduced from Europe and the United States for large-scale planting. Now, with economic development and urban construction, the demand for petunia is increasing. [0003] Plant polyploidy refers to the somatic cells conta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H1/08
CPCA01H1/02A01H1/04A01H1/08
Inventor 魏跃刘艳冯英娜刘叶琼樊开青史红林孙朋朋
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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